Construct: sgRNA BRDN0001145062
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGATGAATCTTGGGTCATTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BUB1 (699)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76770
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
110674120 |
+ |
BUB1 |
NNNTGAATCTTGGGTCATTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
88996561 |
+ |
ZNF804B |
NNNTGAATCTTGAATCATTG |
NGG |
2 |
0.6923 |
Tier II |
| 3 |
NC_000012.12 |
12 |
130368361 |
+ |
PIWIL1 |
NNNTGAATCTTGGGTAAATG |
NGG |
2 |
0.6667 |
Tier II |
| 4 |
NC_000006.12 |
6 |
133531773 |
- |
EYA4 |
NNNTGAATCTTAGCTCATTG |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000007.14 |
7 |
34714368 |
+ |
NPSR1 |
NNNTGAAACTTGGCTCATTG |
NGG |
2 |
0.3429 |
Tier II |
| 6 |
NC_000001.11 |
1 |
46405221 |
+ |
FAAH |
NNNTGCATCTTGGGTCATTT |
NGG |
2 |
0.3182 |
Tier II |
| 7 |
NC_000012.12 |
12 |
92893454 |
- |
EEA1 |
NNNTGAATCTTGGGTGATTG |
NGG |
1 |
0.1538 |
Tier II |
| 8 |
NC_000014.9 |
14 |
67567012 |
- |
GPHN |
NNNTGATTCTTGGGTCCTTG |
NGG |
2 |
0.0772 |
Tier II |
| 9 |
NC_000014.9 |
14 |
67567012 |
- |
PLEKHH1 |
NNNTGATTCTTGGGTCCTTG |
NGG |
2 |
0.0772 |
Tier II |
| 10 |
NC_000007.14 |
7 |
99217487 |
- |
KPNA7 |
NNNTGCATCTTGGGGCATTG |
NGG |
2 |
0.0227 |
Tier II |
| 11 |
NC_000002.12 |
2 |
50795964 |
- |
NRXN1 |
NNNTGAACCTTGGGTCATTG |
NGT |
2 |
0.0161 |
Tier II |
| 12 |
NC_000011.10 |
11 |
65389083 |
+ |
FRMD8 |
NNNTGAATCTTGGGGGATTG |
NGG |
2 |
0.0077 |
Tier II |
| 13 |
NC_000001.11 |
1 |
110166137 |
- |
SLC6A17 |
NNNTGAATCTTGGGTCATTG |
NTC |
2 |
0.0 |
Tier II |
| 14 |
NC_000003.12 |
3 |
7974404 |
- |
LOC101927394 |
NNNTGAATCCTGGGTCATTG |
NGG |
1 |
0.5333 |
Tier III |
| 15 |
NC_000006.12 |
6 |
133531773 |
- |
TARID |
NNNTGAATCTTAGCTCATTG |
NGG |
2 |
0.4 |
Tier III |
| 16 |
NC_000007.14 |
7 |
34714368 |
+ |
NPSR1-AS1 |
NNNTGAAACTTGGCTCATTG |
NGG |
2 |
0.3429 |
Tier III |
| 17 |
NC_000006.12 |
6 |
164010963 |
+ |
LOC105378102 |
NNNTGAGTCTTGGGTCACTG |
NGG |
2 |
0.3025 |
Tier III |
| 18 |
NC_000006.12 |
6 |
23494909 |
+ |
LOC105374976 |
NNNTGAAACTTGGGTCATTG |
NAG |
2 |
0.2074 |
Tier III |
| 19 |
NC_000001.11 |
1 |
110166137 |
- |
SLC6A17-AS1 |
NNNTGAATCTTGGGTCATTG |
NTC |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
127829472 |
+ |
Bub1 |
NNNTGAATCTTGAATCATTG |
NGG |
2 |
0.6923 |
Tier I |
| 2 |
NC_000072.6 |
6 |
12469010 |
+ |
Thsd7a |
NNNTGAATCCTGAGTCATTG |
NGG |
2 |
0.4923 |
Tier I |
| 3 |
NC_000085.6 |
19 |
37963968 |
+ |
Myof |
NNNTGAATCATGGGTAATTG |
NGG |
2 |
0.8571 |
Tier II |
| 4 |
NC_000083.6 |
17 |
30244549 |
- |
Btbd9 |
NNNTGAAATTTGGGTCATTG |
NGG |
2 |
0.7 |
Tier II |
| 5 |
NC_000067.6 |
1 |
129551164 |
+ |
Thsd7b |
NNNTGAATCTTGAGTCAATG |
NGG |
2 |
0.6154 |
Tier II |
| 6 |
NC_000077.6 |
11 |
46369993 |
+ |
Itk |
NNNTGGAACTTGGGTCATTG |
NGG |
2 |
0.5714 |
Tier II |
| 7 |
NC_000069.6 |
3 |
64129768 |
+ |
Vmn2r1 |
NNNTAAATCTTGGGACATTG |
NGG |
2 |
0.5018 |
Tier II |
| 8 |
NC_000069.6 |
3 |
64129768 |
+ |
Vmn2r2 |
NNNTAAATCTTGGGACATTG |
NGG |
2 |
0.5018 |
Tier II |
| 9 |
NC_000067.6 |
1 |
21224881 |
+ |
Tmem14a |
NNNTGCATCTTGGGTAATTG |
NGG |
2 |
0.4545 |
Tier II |
| 10 |
NC_000072.6 |
6 |
137626600 |
- |
Eps8 |
NNNTGTATCTTTGGTCATTG |
NGG |
2 |
0.2747 |
Tier II |
| 11 |
NC_000079.6 |
13 |
102906759 |
- |
Mast4 |
NNNTGAATTTTGTGTCATTG |
NGG |
2 |
0.2625 |
Tier II |
| 12 |
NC_000072.6 |
6 |
71597147 |
- |
Kdm3a |
NNNTGAATCATGTGTCATTG |
NGG |
2 |
0.2571 |
Tier II |
| 13 |
NC_000072.6 |
6 |
46550611 |
+ |
Cntnap2 |
NNNTGAATGTTGGGCCATTG |
NGG |
2 |
0.1688 |
Tier II |
| 14 |
NC_000071.6 |
5 |
86136769 |
+ |
Uba6 |
NNNTGAATCTTGTGTCATTC |
NGG |
2 |
0.1286 |
Tier II |
| 15 |
NC_000086.7 |
X |
100200306 |
+ |
Eda |
NNNTGAGTCTTGGGTCCTTG |
NGG |
2 |
0.1246 |
Tier II |
| 16 |
NC_000069.6 |
3 |
94530533 |
- |
Snx27 |
NNNTGATTCTTGGTTCATTG |
NGG |
2 |
0.1167 |
Tier II |
| 17 |
NC_000084.6 |
18 |
80674276 |
- |
Nfatc1 |
NNNTGAAGCTTGGGTCTTTG |
NGG |
2 |
0.0978 |
Tier II |
| 18 |
NC_000082.6 |
16 |
72133282 |
- |
Robo1 |
NNNAGAATCTTGGGTCATTG |
NGA |
2 |
0.0331 |
Tier II |
| 19 |
NC_000080.6 |
14 |
65747538 |
+ |
Scara5 |
NNNTGAAGCTTGGGTCATTG |
NTG |
2 |
0.0286 |
Tier II |
| 20 |
NC_000069.6 |
3 |
33811064 |
+ |
Ccdc39 |
NNNTGAATCTTGGCTCATTG |
NGC |
2 |
0.0095 |
Tier II |
| 21 |
NC_000069.6 |
3 |
33811064 |
+ |
Ttc14 |
NNNTGAATCTTGGCTCATTG |
NGC |
2 |
0.0095 |
Tier II |
| 22 |
NC_000070.6 |
4 |
155652902 |
- |
Mmp23 |
NNNAGAATCTTGGGTCATTG |
NGT |
2 |
0.0077 |
Tier II |
| 23 |
NC_000072.6 |
6 |
12469010 |
+ |
Gm38583 |
NNNTGAATCCTGAGTCATTG |
NGG |
2 |
0.4923 |
Tier III |
| 24 |
NC_000067.6 |
1 |
21224881 |
+ |
Gm28836 |
NNNTGCATCTTGGGTAATTG |
NGG |
2 |
0.4545 |
Tier III |
| 25 |
NC_000086.7 |
X |
41742447 |
- |
Gm14613 |
NNNTGAATGTTTGGTCATTG |
NGG |
2 |
0.2381 |
Tier III |
| 26 |
NC_000082.6 |
16 |
78226163 |
+ |
Gm31613 |
NNNTGTATCTTGGGCCATTG |
NGG |
2 |
0.1948 |
Tier III |
Other clones with same target sequence:
(none)
