Construct: sgRNA BRDN0001145068
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCCTCCAGAGCGACGATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PNCK (139728)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77328
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
153672184 |
+ |
PNCK |
NNNCTCCAGAGCGACGATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000021.9 |
21 |
46366844 |
- |
PCNT |
NNNCTCCAGAGCGCCGAGGT |
NGG |
2 |
0.0714 |
Tier I |
| 3 |
NC_000007.14 |
7 |
45686179 |
- |
ADCY1 |
NNNCTCCAGAACGAGGATGT |
NGG |
2 |
0.05 |
Tier I |
| 4 |
NC_000011.10 |
11 |
61840434 |
- |
FADS2 |
NNNCTCCAGGGCGATGATGT |
NGG |
2 |
0.037 |
Tier I |
| 5 |
NC_000005.10 |
5 |
144993 |
- |
PLEKHG4B |
NNNCTCCAGAACCACGATGT |
NGG |
2 |
0.4211 |
Tier II |
| 6 |
NC_000008.11 |
8 |
112905595 |
+ |
CSMD3 |
NNNCTCCAGAGCAAAGATGT |
NGG |
2 |
0.2051 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
73658312 |
+ |
Pnck |
NNNCTCCAGAGCCACAATGT |
NGG |
2 |
0.4211 |
Tier I |
| 2 |
NC_000073.6 |
7 |
74504593 |
- |
Slco3a1 |
NNNCTCCAGAGCGCCGATGT |
NGG |
1 |
0.2143 |
Tier I |
| 3 |
NC_000072.6 |
6 |
88576747 |
+ |
Kbtbd12 |
NNNCTCCAGAGCGGCGATGC |
NGG |
2 |
0.0424 |
Tier II |
Other clones with same target sequence:
(none)
