Construct: sgRNA BRDN0001145070
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTTACACAACACTTCGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK1D (1453)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76717
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
82252460 |
+ |
CSNK1D |
NNNTACACAACACTTCGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
213749701 |
+ |
RPS6KC1 |
NNNTGCACTACACTTCGATG |
NGG |
2 |
0.432 |
Tier II |
| 3 |
NC_000009.12 |
9 |
35210889 |
- |
UNC13B |
NNNTACAAAACACTTCTATG |
NGG |
2 |
0.1625 |
Tier II |
| 4 |
NC_000005.10 |
5 |
53017502 |
+ |
ITGA2 |
NNNTCCACAACACTCCGATG |
NGG |
2 |
0.1364 |
Tier II |
| 5 |
NC_000001.11 |
1 |
28833136 |
+ |
OPRD1 |
NNNTTCACAACACTTCTATG |
NGG |
2 |
0.0909 |
Tier II |
| 6 |
NC_000014.9 |
14 |
74474174 |
+ |
SYNDIG1L |
NNNTACACAACACCTCTATG |
NGG |
2 |
0.0714 |
Tier II |
| 7 |
NC_000012.12 |
12 |
6353397 |
- |
SCNN1A |
NNNTACACAACACTTCCCTG |
NGG |
2 |
0.0448 |
Tier II |
| 8 |
NC_000003.12 |
3 |
22106304 |
+ |
ZNF385D |
NNNTACACAACACTTGGATG |
NAG |
2 |
0.0399 |
Tier II |
| 9 |
NC_000003.12 |
3 |
194475828 |
+ |
ATP13A3 |
NNNTACACAACACTTTGATG |
NGC |
2 |
0.0068 |
Tier II |
| 10 |
NC_000006.12 |
6 |
42041018 |
- |
CCND3 |
NNNTACACAACACTTCCATG |
NGC |
2 |
0.0052 |
Tier II |
| 11 |
NC_000006.12 |
6 |
30005568 |
+ |
POLR1HASP |
NNNTACACACCACTTAGATG |
NGG |
2 |
0.3333 |
Tier III |
| 12 |
NC_000006.12 |
6 |
30005568 |
+ |
HCG4P3 |
NNNTACACACCACTTAGATG |
NGG |
2 |
0.3333 |
Tier III |
| 13 |
NC_000006.12 |
6 |
29791207 |
+ |
HCG4 |
NNNTACACAACACTTAGAAG |
NGG |
2 |
0.2857 |
Tier III |
| 14 |
NC_000006.12 |
6 |
29941539 |
+ |
HCG4P5 |
NNNTACACAACACTTAGAAG |
NGG |
2 |
0.2857 |
Tier III |
| 15 |
NC_000006.12 |
6 |
29941539 |
+ |
LOC124901298 |
NNNTACACAACACTTAGAAG |
NGG |
2 |
0.2857 |
Tier III |
| 16 |
NC_000012.12 |
12 |
6353397 |
- |
LOC107984500 |
NNNTACACAACACTTCCCTG |
NGG |
2 |
0.0448 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
36085938 |
+ |
Ccdc58 |
NNNTACATAACACTTAGATG |
NGG |
2 |
0.875 |
Tier II |
| 2 |
NC_000073.6 |
7 |
119876756 |
+ |
Dcun1d3 |
NNNTACATAACACTTCTATG |
NGG |
2 |
0.2188 |
Tier II |
| 3 |
NC_000073.6 |
7 |
119876756 |
+ |
Rexo5 |
NNNTACATAACACTTCTATG |
NGG |
2 |
0.2188 |
Tier II |
| 4 |
NC_000067.6 |
1 |
126732304 |
+ |
Nckap5 |
NNNTACACAACACTTAGCTG |
NGG |
2 |
0.1905 |
Tier II |
| 5 |
NC_000072.6 |
6 |
118115896 |
+ |
Csgalnact2 |
NNNTACACAACACTTCCTTG |
NGG |
2 |
0.1176 |
Tier II |
| 6 |
NC_000075.6 |
9 |
105871539 |
+ |
Col6a5 |
NNNTACACAACAATTCCATG |
NGG |
2 |
0.0905 |
Tier II |
| 7 |
NC_000084.6 |
18 |
39076013 |
- |
Arhgap26 |
NNNTACACAACACTCTGATG |
NGG |
2 |
0.0839 |
Tier II |
| 8 |
NC_000067.6 |
1 |
118509011 |
- |
Clasp1 |
NNNTACACAACACTGCGATG |
NGG |
1 |
0.05 |
Tier II |
| 9 |
NC_000077.6 |
11 |
77277217 |
- |
Ssh2 |
NNNTACACAACACTTGGATG |
NGA |
2 |
0.0107 |
Tier II |
Other clones with same target sequence:
(none)
