Construct: sgRNA BRDN0001145071

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TATTTGAAACTACTAGGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKT3 (10000)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76219

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 243637708 - AKT3 NNNTTGAAACTACTAGGTAA NGG 0 1.0 Tier I
2 NC_000007.14 7 148865877 - EZH2 NNNTTGAAATTACTAGATAA NGG 2 0.8784 Tier II
3 NC_000001.11 1 72263189 - NEGR1 NNNTTGAAAATACTAGATAA NGG 2 0.8089 Tier II
4 NC_000005.10 5 149176365 + ABLIM3 NNNTTAAAACAACTAGGTAA NGG 2 0.75 Tier II
5 NC_000023.11 X 46602738 - SLC9A7 NNNTTGAAACTGCTAAGTAA NGG 2 0.7222 Tier II
6 NC_000008.11 8 38169967 + LSM1 NNNTTTAAACTACTGGGTAA NGG 2 0.4333 Tier II
7 NC_000019.10 19 53252177 - ZNF677 NNNTGGAAACTACTAGGAAA NGG 2 0.4267 Tier II
8 NC_000018.10 18 62943500 - PHLPP1 NNNTTGAAACTAATGGGTAA NGG 2 0.25 Tier II
9 NC_000011.10 11 8391089 + STK33 NNNTTGAGACTACTAGGGAA NGG 2 0.2444 Tier II
10 NC_000008.11 8 115565941 + TRPS1 NNNTTGAAACTTCAAGGTAA NGG 2 0.2063 Tier II
11 NC_000020.11 20 24570656 + SYNDIG1 NNNTTGAAAATACTAGGTAC NGG 2 0.197 Tier II
12 NC_000010.11 10 71223167 + UNC5B NNNTTGAAACGACTAGGTGA NGG 2 0.15 Tier II
13 NC_000001.11 1 118981196 - TBX15 NNNTTGAAACTTCCAGGTAA NGG 2 0.0952 Tier II
14 NC_000009.12 9 117355384 - ASTN2 NNNTTGAAAATACTAGGTAA NTG 2 0.0338 Tier II
15 NC_000005.10 5 143153600 + ARHGAP26 NNNTAGAAACTACTAGGTAA NTG 2 0.0195 Tier II
16 NC_000020.11 20 34435792 + ITCH NNNTTGAAACTACTAGGGAA NGT 2 0.0054 Tier II
17 NC_000008.11 8 28742125 - EXTL3 NNNTTGAAATTACTATGTAA NGG 2 0.0 Tier II
18 NC_000010.11 10 71223167 + LOC112268061 NNNTTGAAACGACTAGGTGA NGG 2 0.15 Tier III
19 NC_000014.9 14 62480563 - LOC105370528 NNNTTGAAACTATTAGGTAA NGT 2 0.0113 Tier III
20 NC_000014.9 14 62480563 - LOC105370529 NNNTTGAAACTATTAGGTAA NGT 2 0.0113 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 177097064 - Akt3 NNNTTGAAACTACTAGGTAA NGG 0 1.0 Tier I
2 NC_000076.6 10 20132210 + Map3k5 NNNTTGAAACTAATAGGTAA NGT 2 0.0062 Tier I
3 NC_000075.6 9 73605688 + Unc13c NNNTTGAAACTACAAGGTAG NGG 2 0.4734 Tier II
4 NC_000083.6 17 35147315 - Bag6 NNNTCGAAACTACTAGGCAA NGG 2 0.4286 Tier II
5 NC_000078.6 12 59178260 + Mia2 NNNTGGAAACTTCTAGGTAA NGG 2 0.2133 Tier II
6 NC_000068.7 2 135138905 + Plcb1 NNNTTGAACCTACTAGGTCA NGG 2 0.1182 Tier II
7 NC_000072.6 6 125673000 + Vwf NNNTTTAAACTACTAGGTAA NTG 2 0.026 Tier II
8 NC_000079.6 13 45686880 + Atxn1 NNNTTGAACCTACTAGGTAA NTG 2 0.0223 Tier II
9 NC_000074.6 8 4286699 - Elavl1 NNNTTGAAACTTCTAGGTAA NGT 2 0.0054 Tier II
10 NC_000080.6 14 118225307 - Gm32045 NNNTTCAATCTACTAGGTAA NGG 2 0.4091 Tier III
11 NC_000067.6 1 21312883 - Gm21909 NNNATGAAACTACTAGGTGA NGG 2 0.1786 Tier III
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Other clones with same target sequence:

(none)