Construct: sgRNA BRDN0001145071
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATTTGAAACTACTAGGTAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AKT3 (10000)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76219
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
243637708 |
- |
AKT3 |
NNNTTGAAACTACTAGGTAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
148865877 |
- |
EZH2 |
NNNTTGAAATTACTAGATAA |
NGG |
2 |
0.8784 |
Tier II |
| 3 |
NC_000001.11 |
1 |
72263189 |
- |
NEGR1 |
NNNTTGAAAATACTAGATAA |
NGG |
2 |
0.8089 |
Tier II |
| 4 |
NC_000005.10 |
5 |
149176365 |
+ |
ABLIM3 |
NNNTTAAAACAACTAGGTAA |
NGG |
2 |
0.75 |
Tier II |
| 5 |
NC_000023.11 |
X |
46602738 |
- |
SLC9A7 |
NNNTTGAAACTGCTAAGTAA |
NGG |
2 |
0.7222 |
Tier II |
| 6 |
NC_000008.11 |
8 |
38169967 |
+ |
LSM1 |
NNNTTTAAACTACTGGGTAA |
NGG |
2 |
0.4333 |
Tier II |
| 7 |
NC_000019.10 |
19 |
53252177 |
- |
ZNF677 |
NNNTGGAAACTACTAGGAAA |
NGG |
2 |
0.4267 |
Tier II |
| 8 |
NC_000018.10 |
18 |
62943500 |
- |
PHLPP1 |
NNNTTGAAACTAATGGGTAA |
NGG |
2 |
0.25 |
Tier II |
| 9 |
NC_000011.10 |
11 |
8391089 |
+ |
STK33 |
NNNTTGAGACTACTAGGGAA |
NGG |
2 |
0.2444 |
Tier II |
| 10 |
NC_000008.11 |
8 |
115565941 |
+ |
TRPS1 |
NNNTTGAAACTTCAAGGTAA |
NGG |
2 |
0.2063 |
Tier II |
| 11 |
NC_000020.11 |
20 |
24570656 |
+ |
SYNDIG1 |
NNNTTGAAAATACTAGGTAC |
NGG |
2 |
0.197 |
Tier II |
| 12 |
NC_000010.11 |
10 |
71223167 |
+ |
UNC5B |
NNNTTGAAACGACTAGGTGA |
NGG |
2 |
0.15 |
Tier II |
| 13 |
NC_000001.11 |
1 |
118981196 |
- |
TBX15 |
NNNTTGAAACTTCCAGGTAA |
NGG |
2 |
0.0952 |
Tier II |
| 14 |
NC_000009.12 |
9 |
117355384 |
- |
ASTN2 |
NNNTTGAAAATACTAGGTAA |
NTG |
2 |
0.0338 |
Tier II |
| 15 |
NC_000005.10 |
5 |
143153600 |
+ |
ARHGAP26 |
NNNTAGAAACTACTAGGTAA |
NTG |
2 |
0.0195 |
Tier II |
| 16 |
NC_000020.11 |
20 |
34435792 |
+ |
ITCH |
NNNTTGAAACTACTAGGGAA |
NGT |
2 |
0.0054 |
Tier II |
| 17 |
NC_000008.11 |
8 |
28742125 |
- |
EXTL3 |
NNNTTGAAATTACTATGTAA |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000010.11 |
10 |
71223167 |
+ |
LOC112268061 |
NNNTTGAAACGACTAGGTGA |
NGG |
2 |
0.15 |
Tier III |
| 19 |
NC_000014.9 |
14 |
62480563 |
- |
LOC105370528 |
NNNTTGAAACTATTAGGTAA |
NGT |
2 |
0.0113 |
Tier III |
| 20 |
NC_000014.9 |
14 |
62480563 |
- |
LOC105370529 |
NNNTTGAAACTATTAGGTAA |
NGT |
2 |
0.0113 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
177097064 |
- |
Akt3 |
NNNTTGAAACTACTAGGTAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000076.6 |
10 |
20132210 |
+ |
Map3k5 |
NNNTTGAAACTAATAGGTAA |
NGT |
2 |
0.0062 |
Tier I |
| 3 |
NC_000075.6 |
9 |
73605688 |
+ |
Unc13c |
NNNTTGAAACTACAAGGTAG |
NGG |
2 |
0.4734 |
Tier II |
| 4 |
NC_000083.6 |
17 |
35147315 |
- |
Bag6 |
NNNTCGAAACTACTAGGCAA |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000078.6 |
12 |
59178260 |
+ |
Mia2 |
NNNTGGAAACTTCTAGGTAA |
NGG |
2 |
0.2133 |
Tier II |
| 6 |
NC_000068.7 |
2 |
135138905 |
+ |
Plcb1 |
NNNTTGAACCTACTAGGTCA |
NGG |
2 |
0.1182 |
Tier II |
| 7 |
NC_000072.6 |
6 |
125673000 |
+ |
Vwf |
NNNTTTAAACTACTAGGTAA |
NTG |
2 |
0.026 |
Tier II |
| 8 |
NC_000079.6 |
13 |
45686880 |
+ |
Atxn1 |
NNNTTGAACCTACTAGGTAA |
NTG |
2 |
0.0223 |
Tier II |
| 9 |
NC_000074.6 |
8 |
4286699 |
- |
Elavl1 |
NNNTTGAAACTTCTAGGTAA |
NGT |
2 |
0.0054 |
Tier II |
| 10 |
NC_000080.6 |
14 |
118225307 |
- |
Gm32045 |
NNNTTCAATCTACTAGGTAA |
NGG |
2 |
0.4091 |
Tier III |
| 11 |
NC_000067.6 |
1 |
21312883 |
- |
Gm21909 |
NNNATGAAACTACTAGGTGA |
NGG |
2 |
0.1786 |
Tier III |
Other clones with same target sequence:
(none)
