Construct: sgRNA BRDN0001145081

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATAGCATTGTATTTACCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK1G3 (1456)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77362

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 123573428 - CSNK1G3 NNNGCATTGTATTTACCACA NGG 0 1.0 Tier I
2 NC_000015.10 15 64216676 + CSNK1G1 NNNGCATTATATTTCCCACA NGG 2 0.1753 Tier I
3 NC_000015.10 15 24846377 - SNRPN NNNGCAATGTATATACCACA NGG 2 0.6058 Tier II
4 NC_000011.10 11 18361921 + GTF2H1 NNNGCATTGTATTTAAAACA NGG 2 0.4667 Tier II
5 NC_000006.12 6 122625414 + PKIB NNNCCATTGTATCTACCACA NGG 2 0.418 Tier II
6 NC_000002.12 2 172956338 - RAPGEF4 NNNTCATTGTATTTACCACA NGG 1 0.3636 Tier II
7 NC_000006.12 6 142309330 - ADGRG6 NNNGCATTTTATTTGCCACA NGG 2 0.3467 Tier II
8 NC_000005.10 5 113520598 + YTHDC2 NNNGCCTGGTATTTACCACA NGG 2 0.3333 Tier II
9 NC_000012.12 12 65853962 + HMGA2 NNNGCACTGTATTTACAACA NGG 2 0.3208 Tier II
10 NC_000003.12 3 27313675 - NEK10 NNNGCATTGTATATACCATA NGG 2 0.3195 Tier II
11 NC_000007.14 7 116542128 + CAV1 NNNGCATTGTATATACCAAA NGG 2 0.2967 Tier II
12 NC_000010.11 10 66951053 + CTNNA3 NNNGCACTGTATTTATCACA NGG 2 0.2115 Tier II
13 NC_000010.11 10 66951053 + LRRTM3 NNNGCACTGTATTTATCACA NGG 2 0.2115 Tier II
14 NC_000006.12 6 157537972 + ZDHHC14 NNNGCCTTGTCTTTACCACA NGG 2 0.1818 Tier II
15 NC_000005.10 5 84229210 - EDIL3 NNNGCATGGTATTTTCCACA NGG 2 0.1467 Tier II
16 NC_000002.12 2 45657002 + PRKCE NNNGCATTGCATTTAGCACA NGG 2 0.0821 Tier II
17 NC_000001.11 1 182878714 + DHX9 NNNGCATTGTATTTACCAGT NGG 2 0.075 Tier II
18 NC_000001.11 1 165216412 - LMX1A NNNGCATTGTTTTTACCCCA NGG 2 0.0586 Tier II
19 NC_000001.11 1 71643777 - NEGR1 NNNGCATTCTATTTACCACA NGA 2 0.0374 Tier II
20 NC_000020.11 20 14976861 - MACROD2 NNNGCATTGTATTTGCCACA NTG 2 0.0253 Tier II
21 NC_000005.10 5 150710838 - DCTN4 NNNGCATTGTATTTACCACC NGA 2 0.0158 Tier II
22 NC_000005.10 5 58674605 - RAB3C NNNGTATTGTATTGACCACA NGG 2 0.0 Tier II
23 NC_000011.10 11 25869445 - LINC02699 NNNGCATTGTGTTTACCACA NGG 1 0.65 Tier III
24 NC_000015.10 15 24846377 - SNHG14 NNNGCAATGTATATACCACA NGG 2 0.6058 Tier III
25 NC_000013.11 13 59574221 - LOC107984625 NNNGCATTGTGGTTACCACA NGG 2 0.325 Tier III
26 NC_000012.12 12 65853962 + HMGA2-AS1 NNNGCACTGTATTTACAACA NGG 2 0.3208 Tier III
27 NC_000010.11 10 66951053 + LOC101928961 NNNGCACTGTATTTATCACA NGG 2 0.2115 Tier III
28 NC_000013.11 13 89415528 - LOC107984621 NNNGCATTGTATTTAGCACA NGG 1 0.1538 Tier III
29 NC_000001.11 1 165216412 - LMX1A-AS1 NNNGCATTGTTTTTACCCCA NGG 2 0.0586 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 53917010 - Csnk1g3 NNNGCGTTGTATTTACCACA NGG 1 0.7143 Tier I
2 NC_000073.6 7 44192323 - Klk1b24 NNNGCATTGGGTTTACCACA NGG 2 0.325 Tier I
3 NC_000072.6 6 23072996 - Aass NNNGCATTGTATTTAACACA NAG 2 0.2593 Tier I
4 NC_000075.6 9 65999433 - Csnk1g1 NNNGCATTGTACTTCCCACA NGG 2 0.2584 Tier I
5 NC_000070.6 4 145003647 + Vps13d NNNACATTGTATTTACCACA NGG 1 0.9 Tier II
6 NC_000076.6 10 127476491 + R3hdm2 NNNGCATTGTATTTACAACT NGG 2 0.28 Tier II
7 NC_000072.6 6 64220354 - Grid2 NNNGCATTGTATCTACCACC NGG 2 0.1794 Tier II
8 NC_000070.6 4 114500832 - Trabd2b NNNTCATTGTATTTACCATA NGG 2 0.1678 Tier II
9 NC_000083.6 17 27938689 - Anks1 NNNGCATTCTATTTAGCACA NGG 2 0.0828 Tier II
10 NC_000068.7 2 158455131 + Ralgapb NNNGCATTGTATTTTTCACA NGG 2 0.0615 Tier II
11 NC_000078.6 12 71863004 - Daam1 NNNGCATAGTATTTACCACA NGA 2 0.0556 Tier II
12 NC_000069.6 3 10418943 - Snx16 NNNGCATTGTATTTACTACA NTG 2 0.0182 Tier II
13 NC_000079.6 13 74267425 + Ahrr NNNGCATTGAATTTACCACA NGT 2 0.0138 Tier II
14 NC_000079.6 13 20538997 + Elmo1 NNNGCATTGTATTTACCAAA NGT 2 0.0069 Tier II
15 NC_000075.6 9 24590896 + Dpy19l2 NNNGCATTGTATTGAGCACA NGG 2 0.0 Tier II
16 NC_000067.6 1 28864225 + Gm45942 NNNGCATTGTATTTACCATA NGT 2 0.0074 Tier III
Download CSV

Other clones with same target sequence:

(none)