Construct: sgRNA BRDN0001145095
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGCAATAATAGGGATTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LTK (4058)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77597
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
41507192 |
+ |
LTK |
NNNGCAATAATAGGGATTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
128548194 |
- |
TMEM132C |
NNNGCAATTTTAGGGATTGG |
NGG |
2 |
0.5294 |
Tier II |
| 3 |
NC_000013.11 |
13 |
91404070 |
- |
GPC5 |
NNNAAAATAATAGGGATTGG |
NGG |
2 |
0.5143 |
Tier II |
| 4 |
NC_000015.10 |
15 |
25855190 |
- |
ATP10A |
NNNGCAATATTTGGGATTGG |
NGG |
2 |
0.2941 |
Tier II |
| 5 |
NC_000023.11 |
X |
23856424 |
- |
APOO |
NNNCCAATAATAGGGATTGC |
NGG |
2 |
0.2269 |
Tier II |
| 6 |
NC_000021.9 |
21 |
37839937 |
+ |
KCNJ6 |
NNNGCCATAATAGGGATTGC |
NGG |
2 |
0.1948 |
Tier II |
| 7 |
NC_000004.12 |
4 |
153212374 |
+ |
TRIM2 |
NNNGGAATAATAGGCATTGG |
NGG |
2 |
0.1636 |
Tier II |
| 8 |
NC_000010.11 |
10 |
77071967 |
- |
KCNMA1 |
NNNGCAATAAGAGGGATGGG |
NGG |
2 |
0.1333 |
Tier II |
| 9 |
NC_000012.12 |
12 |
125312016 |
+ |
TMEM132B |
NNNGCAGTAATAGGGAGTGG |
NGG |
2 |
0.083 |
Tier II |
| 10 |
NC_000002.12 |
2 |
229483115 |
+ |
DNER |
NNNGCAATAATAGGGAGAGG |
NGG |
2 |
0.0784 |
Tier II |
| 11 |
NC_000023.11 |
X |
122027382 |
+ |
UBE2V1P16 |
NNNGCAAGAATAGGGATTGG |
NGA |
2 |
0.0509 |
Tier II |
| 12 |
NC_000004.12 |
4 |
98675337 |
+ |
LOC112267901 |
NNNGTAATAATAAGGATTGG |
NGG |
2 |
0.5874 |
Tier III |
| 13 |
NC_000017.11 |
17 |
30313195 |
- |
LOC105371721 |
NNNACAATAATAGGGAATGG |
NGG |
2 |
0.48 |
Tier III |
| 14 |
NC_000002.12 |
2 |
51874839 |
+ |
NRXN1-DT |
NNNGGAATAATAGGGATTAG |
NGG |
2 |
0.4286 |
Tier III |
| 15 |
NC_000004.12 |
4 |
64984396 |
+ |
LINC02232 |
NNNGAAATAATAGGGAATGG |
NGG |
2 |
0.3048 |
Tier III |
| 16 |
NC_000002.12 |
2 |
217581462 |
- |
DIRC3 |
NNNGCAAGAATATGGATTGG |
NGG |
2 |
0.22 |
Tier III |
| 17 |
NC_000012.12 |
12 |
40180233 |
- |
LRRK2-DT |
NNNGAAATAATAGGGATTGG |
NAG |
2 |
0.1481 |
Tier III |
| 18 |
NC_000018.10 |
18 |
4470769 |
- |
ELOCP27 |
NNNGCAAGAATAGGGAGTGG |
NGG |
2 |
0.0863 |
Tier III |
| 19 |
NC_000004.12 |
4 |
18581655 |
+ |
LOC105374510 |
NNNGCAATAATAGAGCTTGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
4859864 |
- |
Tcea1 |
NNNGAAAAAATAGGGATTGG |
NGG |
2 |
0.4571 |
Tier II |
| 2 |
NC_000083.6 |
17 |
10531445 |
+ |
Pacrg |
NNNCCAATAATTGGGATTGG |
NGG |
2 |
0.1765 |
Tier II |
| 3 |
NC_000077.6 |
11 |
47491723 |
- |
Sgcd |
NNNGGAATAATAGGGGTTGG |
NGG |
2 |
0.1154 |
Tier II |
| 4 |
NC_000068.7 |
2 |
54795296 |
- |
Galnt13 |
NNNGCAATCATAGGGAGTGG |
NGG |
2 |
0.0672 |
Tier II |
| 5 |
NC_000078.6 |
12 |
21031796 |
- |
Gm5977 |
NNNACAATAATAGGAATTGG |
NGG |
2 |
0.8471 |
Tier III |
| 6 |
NC_000083.6 |
17 |
10531445 |
+ |
Gm16168 |
NNNCCAATAATTGGGATTGG |
NGG |
2 |
0.1765 |
Tier III |
| 7 |
NC_000083.6 |
17 |
10531445 |
+ |
Gm52294 |
NNNCCAATAATTGGGATTGG |
NGG |
2 |
0.1765 |
Tier III |
| 8 |
NC_000070.6 |
4 |
41648432 |
- |
Gm12405 |
NNNGCAGTAATAGGGTTTGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
