Construct: sgRNA BRDN0001145106

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACAAAAATGTAGGATGAACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOL9 (79707)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76978

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 6549595 + NOL9 NNNAAAATGTAGGATGAACC NGG 0 1.0 Tier I
2 NC_000003.12 3 11428899 - ATG7 NNNAAAATGTAAGATAAACC NGG 2 0.9333 Tier II
3 NC_000016.10 16 70847526 - HYDIN NNNAAAATGAAGGATAAACC NGG 2 0.8571 Tier II
4 NC_000015.10 15 29439249 + ENTREP2 NNNAGAATGTAAGATGAACC NGG 2 0.672 Tier II
5 NC_000023.11 X 75141823 + ABCB7 NNNAAAATATAAGATGAACC NGG 2 0.6 Tier II
6 NC_000002.12 2 238452203 + ASB1 NNNAAGATGTGGGATGAACC NGG 2 0.4643 Tier II
7 NC_000010.11 10 49877562 + PARG NNNAAAATGTAAGATGAATC NGG 2 0.4308 Tier II
8 NC_000009.12 9 39105671 - CNTNAP3 NNNAAAATGTAAGATGAGCC NGG 2 0.3733 Tier II
9 NC_000003.12 3 78889268 + ROBO1 NNNAAAATGAAGGATGAGCC NGG 2 0.3429 Tier II
10 NC_000013.11 13 51664485 + WDFY2 NNNAAAAGGTAGGGTGAACC NGG 2 0.3422 Tier II
11 NC_000008.11 8 134481155 + ZFAT NNNAAAATGTACCATGAACC NGG 2 0.2229 Tier II
12 NC_000017.11 17 39316478 + FBXL20 NNNAAAATGTACGATGAGCC NGG 2 0.2118 Tier II
13 NC_000002.12 2 8749407 - KIDINS220 NNNAAAATGGAGCATGAACC NGG 2 0.2105 Tier II
14 NC_000003.12 3 76324178 + ROBO2 NNNAAATTGTAGGATGAAAC NGG 2 0.1875 Tier II
15 NC_000009.12 9 123091500 + RABGAP1 NNNAAAATGTAGGATGGACC NGG 1 0.1765 Tier II
16 NC_000002.12 2 49101202 + FSHR NNNAAAATTTTGGATGAACC NGG 2 0.1641 Tier II
17 NC_000010.11 10 1555567 + ADARB2 NNNAAAATGAAGGATGGACC NGG 2 0.1513 Tier II
18 NC_000002.12 2 230498292 + SP100 NNNAAAATCTAGGATGAACC NAG 2 0.1396 Tier II
19 NC_000002.12 2 177777601 - PDE11A NNNAAAATGTATGATGAACC NAG 2 0.0997 Tier II
20 NC_000003.12 3 12376683 + PPARG NNNAAAAGGTAGGATGTACC NGG 2 0.0978 Tier II
21 NC_000008.11 8 102029180 + NCALD NNNAAAATGTAGGGTGTACC NGG 2 0.0622 Tier II
22 NC_000003.12 3 40231436 + MYRIP NNNAAAATGTAGAATGAACC NTG 2 0.036 Tier II
23 NC_000008.11 8 71449909 - EYA1 NNNAAAATGTAGGATGAAGC NAG 2 0.0324 Tier II
24 NC_000012.12 12 40410588 - MUC19 NNNTAAATGTAGGATGAACC NTG 2 0.0248 Tier II
25 NC_000005.10 5 159282730 + UBLCP1 NNNAAAATGTAGGAGGAAAC NGG 2 0.0214 Tier II
26 NC_000012.12 12 19277519 - PLEKHA5 NNNAAAATGTAGGAGGAACC NAG 2 0.013 Tier II
27 NC_000005.10 5 11506466 + CTNND2 NNNAAAATGTAGGATGAACA NGT 2 0.0081 Tier II
28 NC_000018.10 18 43055577 - RIT2 NNNAAAATGTAGTATGAACC NGC 2 0.0067 Tier II
29 NC_000002.12 2 104669466 - LOC105373526 NNNAAAACGTAGGATGATCC NGG 2 0.5 Tier III
30 NC_000002.12 2 238452203 + LOC107986003 NNNAAGATGTGGGATGAACC NGG 2 0.4643 Tier III
31 NC_000010.11 10 45908430 + PARGP1 NNNAAAATGTAAGATGAATC NGG 2 0.4308 Tier III
32 NC_000010.11 10 45908430 + PARGP1-AGAP4 NNNAAAATGTAAGATGAATC NGG 2 0.4308 Tier III
33 NC_000015.10 15 35793199 - DPH6-DT NNNAAAATACAGGATGAACC NGG 2 0.3429 Tier III
34 NC_000006.12 6 81768146 - LOC105377873 NNNAAAGTGTAGGATGAATC NGG 2 0.3258 Tier III
35 NC_000012.12 12 75579392 - LOC105369844 NNNAAAGTGTAGGATGAATC NGG 2 0.3258 Tier III
36 NC_000004.12 4 16317946 - LOC124900674 NNNAACATGTATGATGAACC NGG 2 0.1748 Tier III
37 NC_000010.11 10 1555567 + ADARB2-AS1 NNNAAAATGAAGGATGGACC NGG 2 0.1513 Tier III
38 NC_000001.11 1 187097084 + LINC01036 NNNAAAAGGTAGGATGACCC NGG 2 0.1397 Tier III
39 NC_000002.12 2 230498292 + LOC101928816 NNNAAAATCTAGGATGAACC NAG 2 0.1396 Tier III
40 NC_000007.14 7 25999582 + LOC105375199 NNNAAAATATAGGCTGAACC NGG 2 0.1378 Tier III
41 NC_000015.10 15 77531423 + LOC101929457 NNNAAAATGGAGGATGAAGC NGG 2 0.0625 Tier III
42 NC_000003.12 3 40231436 + EIF1B-AS1 NNNAAAATGTAGAATGAACC NTG 2 0.036 Tier III
43 NC_000002.12 2 145111600 + LOC100505498 NNNAAAATGTAGGAGGAACA NGG 2 0.025 Tier III
44 NC_000012.12 12 40410588 - LOC105369736 NNNTAAATGTAGGATGAACC NTG 2 0.0248 Tier III
45 NC_000012.12 12 19277519 - LOC124902892 NNNAAAATGTAGGAGGAACC NAG 2 0.013 Tier III
46 NC_000004.12 4 68906508 - LOC101930041 NNNAAAATGTAGGACCAACC NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 107489593 - Syt9 NNNAAAATGTAAGATGAACC NGG 1 0.9333 Tier II
2 NC_000068.7 2 157292965 + Rpn2 NNNAAAATGAAGAATGAACC NGG 2 0.7912 Tier II
3 NC_000068.7 2 84981196 - Prg2 NNNAAAATTTAGGATGAACC NGG 1 0.5333 Tier II
4 NC_000070.6 4 128448132 - Csmd2 NNNAAAATGTAGCATGAACC NGG 1 0.4211 Tier II
5 NC_000084.6 18 33876110 + Epb41l4a NNNAAAAGTTAGGATGAACC NGG 2 0.3911 Tier II
6 NC_000068.7 2 125165269 - Slc12a1 NNNCAAATGTAGGATGAACC NGG 1 0.3529 Tier II
7 NC_000078.6 12 74936422 - Kcnh5 NNNAAAATGTAAGATGAACT NGG 2 0.28 Tier II
8 NC_000072.6 6 54686016 + Mturn NNNAAAATGTGGGATGAGCC NGG 2 0.26 Tier II
9 NC_000068.7 2 181468019 + Zbtb46 NNNACCATGTAGGATGAACC NGG 2 0.2273 Tier II
10 NC_000076.6 10 106555307 + Ppfia2 NNNAGAATGTAGGATGAACT NGG 2 0.216 Tier II
11 NC_000076.6 10 84970522 + Ric8b NNNAAAATGTAGGAAGAAGC NGG 2 0.0724 Tier II
12 NC_000080.6 14 78621550 + Dgkh NNNAAAATGTAGGTTGTACC NGG 2 0.0711 Tier II
13 NC_000069.6 3 107454096 + Kcnc4 NNNAAAATGTAGCATGAAGC NGG 2 0.0526 Tier II
14 NC_000072.6 6 40641875 + Mgam NNNAAAGTGTAGGAGGAACC NGG 2 0.0353 Tier II
15 NC_000070.6 4 14671378 + Lrrc69 NNNAAAATGTAGGAAGAACC NTG 2 0.0226 Tier II
16 NC_000078.6 12 4422128 + Ncoa1 NNNAAAATGTAGGATAAACC NGT 2 0.0161 Tier II
17 NC_000073.6 7 85874304 - Vmn2r73 NNNAAAATGTAGGATGGACC NGA 2 0.0123 Tier II
18 NC_000082.6 16 81594653 - Ncam2 NNNAAAATGTAGGATGAATC NGT 2 0.0074 Tier II
19 NC_000081.6 15 65883008 + Oc90 NNNAAAATGTAGGATGACCC NTG 2 0.0074 Tier II
20 NC_000078.6 12 24812779 - 2900060N12Rik NNNGAAATGCAGGATGAACC NGG 2 0.3333 Tier III
21 NC_000076.6 10 106555307 + 4930532I03Rik NNNAGAATGTAGGATGAACT NGG 2 0.216 Tier III
22 NC_000072.6 6 41535350 - Tcrb NNNAAACTGTAGGATGACCC NGG 2 0.0833 Tier III
23 NC_000074.6 8 41984729 + 2810404M03Rik NNNAAAATGTAGGATGACAC NGG 2 0.0816 Tier III
24 NC_000076.6 10 84970522 + Gm34633 NNNAAAATGTAGGAAGAAGC NGG 2 0.0724 Tier III
25 NC_000074.6 8 88027033 + Gm39217 NNNAAAATGGAGGATGAAGC NGG 2 0.0625 Tier III
26 NC_000082.6 16 89128551 + Gm41487 NNNAAAATGTAGGATGGAGC NGG 2 0.0221 Tier III
27 NC_000073.6 7 71220437 - 6030442E23Rik NNNAAAATGAAGGATGAACC NGC 2 0.019 Tier III
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Other clones with same target sequence:

(none)