Construct: sgRNA BRDN0001145110
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCACCACGCACAAATAGCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ULK4 (54986)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76009
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
41896883 |
+ |
ULK4 |
NNNCCACGCACAAATAGCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
126497089 |
+ |
GRM8 |
NNNCCATACACAAATAGCAA |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000015.10 |
15 |
30646235 |
- |
ARHGAP11B |
NNNCCATGCACAAATAGCAA |
NGG |
1 |
0.8125 |
Tier II |
| 4 |
NC_000008.11 |
8 |
58841089 |
- |
TOX |
NNNCCACCCACAAATAGTAA |
NGG |
2 |
0.3956 |
Tier II |
| 5 |
NC_000007.14 |
7 |
134962638 |
- |
CALD1 |
NNNCTACCCACAAATAGCAA |
NGG |
2 |
0.3916 |
Tier II |
| 6 |
NC_000015.10 |
15 |
30381764 |
+ |
CHRFAM7A |
NNNCCACTCACAAACAGCAA |
NGG |
2 |
0.1705 |
Tier II |
| 7 |
NC_000003.12 |
3 |
29411816 |
+ |
RBMS3 |
NNNCCACTCACAAATAGCAA |
NTG |
2 |
0.0244 |
Tier II |
| 8 |
NC_000003.12 |
3 |
136703646 |
+ |
STAG1 |
NNNCCACACACAAATAGCAA |
NGT |
2 |
0.0161 |
Tier II |
| 9 |
NC_000012.12 |
12 |
80106165 |
- |
OTOGL |
NNNCCACGCACAAATACCAA |
NTG |
2 |
0.0092 |
Tier II |
| 10 |
NC_000007.14 |
7 |
126497089 |
+ |
LOC101928357 |
NNNCCATACACAAATAGCAA |
NGG |
2 |
0.8125 |
Tier III |
| 11 |
NC_000015.10 |
15 |
30646235 |
- |
LOC100288637 |
NNNCCATGCACAAATAGCAA |
NGG |
1 |
0.8125 |
Tier III |
| 12 |
NC_000015.10 |
15 |
30115528 |
- |
ULK4P3 |
NNNCCACTCACAAACAGCAA |
NGG |
2 |
0.1705 |
Tier III |
| 13 |
NC_000015.10 |
15 |
30584514 |
- |
ULK4P2 |
NNNCCACTCACAAACAGCAA |
NGG |
2 |
0.1705 |
Tier III |
| 14 |
NC_000015.10 |
15 |
32423090 |
+ |
ULK4P1 |
NNNCCACTCACAAACAGCAA |
NGG |
2 |
0.1705 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
90313587 |
+ |
Nrxn3 |
NNNCAATGCACAAATAGCAA |
NGG |
2 |
0.4643 |
Tier II |
| 2 |
NC_000069.6 |
3 |
14163065 |
+ |
Ralyl |
NNNCCACACAGAAATAGCAA |
NGG |
2 |
0.25 |
Tier II |
| 3 |
NC_000073.6 |
7 |
65028973 |
+ |
Fam189a1 |
NNNCCACCCACAAATAGCAA |
NGA |
2 |
0.0427 |
Tier II |
| 4 |
NC_000077.6 |
11 |
76590790 |
+ |
Abr |
NNNGCACGCACAAATAGCAA |
NGC |
2 |
0.0111 |
Tier II |
| 5 |
NC_000068.7 |
2 |
102954686 |
- |
Gm34483 |
NNNCCAAACACAAATAGCAA |
NGG |
2 |
0.75 |
Tier III |
Other clones with same target sequence:
(none)
