Construct: sgRNA BRDN0001145116
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAATAATCCGTCATTCCTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDGFRA (5156)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75491
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
54263693 |
- |
PDGFRA |
NNNTAATCCGTCATTCCTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
20757747 |
- |
FOCAD |
NNNTGATCCTTCATTCCTAG |
NGG |
2 |
0.585 |
Tier II |
| 3 |
NC_000004.12 |
4 |
26715831 |
+ |
TBC1D19 |
NNNTAGTCCTTCATTCCTAG |
NGG |
2 |
0.5804 |
Tier II |
| 4 |
NC_000005.10 |
5 |
61023198 |
+ |
NDUFAF2 |
NNNTAATCTGTCATTCCTTG |
NGG |
2 |
0.4712 |
Tier II |
| 5 |
NC_000012.12 |
12 |
111129704 |
- |
CUX2 |
NNNTAATCCTGCATTCCTAG |
NGG |
2 |
0.325 |
Tier II |
| 6 |
NC_000001.11 |
1 |
210475825 |
- |
HHAT |
NNNCAATCCCTCATTCCTAG |
NGG |
2 |
0.2588 |
Tier II |
| 7 |
NC_000012.12 |
12 |
20813829 |
- |
SLCO1B3 |
NNNTAATCCATCATTCCTAG |
NAG |
2 |
0.242 |
Tier II |
| 8 |
NC_000006.12 |
6 |
93644406 |
- |
LOC105377899 |
NNNTCATCCGACATTCCTAG |
NGG |
2 |
0.375 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
108852055 |
+ |
Celsr3 |
NNNTAATCAGACATTCCTAG |
NGG |
2 |
0.6429 |
Tier II |
| 2 |
NC_000079.6 |
13 |
3943352 |
+ |
Ucn3 |
NNNCAATCCTTCATTCCTAG |
NGG |
2 |
0.5257 |
Tier II |
| 3 |
NC_000081.6 |
15 |
79092768 |
- |
Eif3l |
NNNTAATCCAGCATTCCTAG |
NGG |
2 |
0.3733 |
Tier II |
| 4 |
NC_000067.6 |
1 |
39457364 |
- |
Tbc1d8 |
NNNTTATCCGTCATTCCTTG |
NGG |
2 |
0.1958 |
Tier II |
Other clones with same target sequence:
(none)
