Construct: sgRNA BRDN0001145124

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTTGTATGCTGGGAATGACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TGFBR3 (7049)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76290

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 91720023 + TGFBR3 NNNGTATGCTGGGAATGACA NGG 0 1.0 Tier I
2 NC_000011.10 11 132146353 + NTM NNNCTATGCTGGGAATGACA NGT 2 0.0085 Tier I
3 NC_000012.12 12 116217978 - MED13L NNNGTGTGCTGGGAAAGACA NGG 2 0.6494 Tier II
4 NC_000022.11 22 49856931 - ZBED4 NNNGTTTGCTGGGAAAGACA NGG 2 0.6494 Tier II
5 NC_000016.10 16 89402496 + ANKRD11 NNNGCATCCTGGGAATGACA NGG 2 0.6154 Tier II
6 NC_000016.10 16 89402496 + LOC128462377 NNNGCATCCTGGGAATGACA NGG 2 0.6154 Tier II
7 NC_000019.10 19 54810645 + KIR2DL4 NNNAAATGCTGGGAATGACA NGG 2 0.45 Tier II
8 NC_000005.10 5 123609712 + CSNK1G3 NNNGTGTTCTGGGAATGACA NGG 2 0.4464 Tier II
9 NC_000005.10 5 7473648 + ADCY2 NNNGTACCCTGGGAATGACA NGG 2 0.4231 Tier II
10 NC_000013.11 13 96276073 - HS6ST3 NNNGCATGCTGGGAATGGCA NGG 2 0.4 Tier II
11 NC_000013.11 13 96276092 - HS6ST3 NNNGCATGCTGGGAATGGCA NGG 2 0.4 Tier II
12 NC_000001.11 1 240846205 - RGS7 NNNTTATACTGGGAATGACA NGG 2 0.3636 Tier II
13 NC_000008.11 8 101903905 + NCALD NNNGTATGCTGGGTGTGACA NGG 2 0.3467 Tier II
14 NC_000012.12 12 2200678 + CACNA1C NNNTTATTCTGGGAATGACA NGG 2 0.2273 Tier II
15 NC_000011.10 11 14795801 + PDE3B NNNGTATGCTGTGAATGATA NGG 2 0.1775 Tier II
16 NC_000009.12 9 36232127 - GNE NNNATATGCTGGGAATGAGA NGG 2 0.1125 Tier II
17 NC_000009.12 9 36232982 + GNE NNNATATGCTGGGAATGAGA NGG 2 0.1125 Tier II
18 NC_000008.11 8 71387267 + EYA1 NNNGTAAGCTGGGAATGACA NGC 2 0.0194 Tier II
19 NC_000002.12 2 168858157 - NOSTRIN NNNGTATGCTGGTAATGACA NTG 2 0.0117 Tier II
20 NC_000005.10 5 98106531 - LINC01846 NNNGTTTACTGGGAATGACA NGG 2 0.7143 Tier III
21 NC_000022.11 22 49856931 - LOC124905147 NNNGTTTGCTGGGAAAGACA NGG 2 0.6494 Tier III
22 NC_000004.12 4 127094809 + LOC102724210 NNNGTTTCCTGGGAATGACA NGG 2 0.4396 Tier III
23 NC_000005.10 5 4147995 - LINC02063 NNNGTGTGCTGGGGATGACA NGG 2 0.3333 Tier III
24 NC_000018.10 18 23965550 + LINC02958 NNNGTATGCTGGGAAAGACA NAG 2 0.2357 Tier III
25 NC_000015.10 15 25185751 + SNHG14 NNNGGATGCTGGGAATCACA NGG 2 0.1506 Tier III
26 NC_000005.10 5 109455318 + LOC105379117 NNNGTATGCTGGCAATGACC NGG 2 0.0957 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 81351771 - Cpeb1 NNNGTATGCTGGGATTGACA NAG 2 0.0519 Tier I
2 NC_000075.6 9 28675205 + Opcml NNNCTATGCTGGGAATGACA NGT 2 0.0085 Tier I
3 NC_000069.6 3 68036863 + Iqcj NNNGCATGCTGGGAATAACA NGG 2 0.9333 Tier II
4 NC_000069.6 3 68036863 + Iqschfp NNNGCATGCTGGGAATAACA NGG 2 0.9333 Tier II
5 NC_000081.6 15 9101863 - Nadk2 NNNGCATGCTGGGAAAGACA NGG 2 0.9091 Tier II
6 NC_000067.6 1 89893970 + Agap1 NNNGTGTGCAGGGAATGACA NGG 2 0.6122 Tier II
7 NC_000080.6 14 117949190 - Gpc6 NNNGGATGCAGGGAATGACA NGG 2 0.5486 Tier II
8 NC_000072.6 6 83649125 - Clec4f NNNGTGTGCTGGGAGTGACA NGG 2 0.4643 Tier II
9 NC_000075.6 9 56647881 - Lingo1 NNNGTTTGCTGGGAGTGACA NGG 2 0.4643 Tier II
10 NC_000077.6 11 59752243 - Mprip NNNGTATGCAGGGAATGTCA NGG 2 0.4286 Tier II
11 NC_000070.6 4 128603122 - Zscan20 NNNGTATGCTGTGAATGACA NGG 1 0.3846 Tier II
12 NC_000077.6 11 61029441 - Kcnj12 NNNGAATTCTGGGAATGACA NGG 2 0.3125 Tier II
13 NC_000079.6 13 104067772 + Nln NNNGAATGCTGGGAATGTCA NGG 2 0.25 Tier II
14 NC_000068.7 2 158185666 - D630003M21Rik NNNGTCTGCTGGGTATGACA NGG 2 0.2424 Tier II
15 NC_000081.6 15 99713159 - Smarcd1 NNNCTCTGCTGGGAATGACA NGG 2 0.2406 Tier II
16 NC_000079.6 13 12252435 - Mtr NNNGAATGCTGGGAATGAAA NGG 2 0.2143 Tier II
17 NC_000083.6 17 27155963 - Ip6k3 NNNGTCTGCTGGGGATGACA NGG 2 0.2121 Tier II
18 NC_000080.6 14 79113850 + Vwa8 NNNTAATGCTGGGAATGACA NGG 2 0.1818 Tier II
19 NC_000071.6 5 20576811 - Magi2 NNNGTATGCTGTGGATGACA NGG 2 0.1795 Tier II
20 NC_000070.6 4 127180393 - Dlgap3 NNNGTATCCTGGGACTGACA NGG 2 0.1678 Tier II
21 NC_000073.6 7 29851800 - Zfp940 NNNGTATGCTGTGAATGAAA NGG 2 0.1648 Tier II
22 NC_000068.7 2 145231435 - Slc24a3 NNNGGATGCTGGGAATGCCA NGG 2 0.1219 Tier II
23 NC_000082.6 16 18081141 - Prodh NNNGTATGCTGGGACTGGCA NGG 2 0.1091 Tier II
24 NC_000072.6 6 31416238 - Mkln1 NNNGTCTGCTGGGCATGACA NGG 2 0.0974 Tier II
25 NC_000073.6 7 111044509 + Ctr9 NNNTTATGCTGGGAATTACA NGG 2 0.0909 Tier II
26 NC_000079.6 13 99980892 + Mccc2 NNNGTATTCTGGGAATGACA NGA 2 0.0434 Tier II
27 NC_000086.7 X 100241976 - Eda NNNGTATGCTGGGAAGGACA NGA 2 0.024 Tier II
28 NC_000073.6 7 45361201 - Ppfia3 NNNGTAAGCTGGGAATGACA NGC 2 0.0194 Tier II
29 NC_000078.6 12 79691081 - Rad51b NNNGTATGCTGGGAATCACA NGA 2 0.0163 Tier II
30 NC_000074.6 8 23731175 - Zmat4 NNNGTATGCTGGGAATGAGA NGA 2 0.0087 Tier II
31 NC_000084.6 18 82689075 + Zfp236 NNNGTATGCTGGGCATGACA NTG 2 0.0083 Tier II
32 NC_000072.6 6 6852053 + Dlx6os1 NNNCTAAGCTGGGAATGACA NGG 2 0.4632 Tier III
33 NC_000079.6 13 28541719 + 2610307P16Rik NNNTTATGCTGGGAAAGACA NGG 2 0.3306 Tier III
34 NC_000079.6 13 54872421 - Gm40959 NNNGTAAGCTGGTAATGACA NGG 2 0.2625 Tier III
35 NC_000075.6 9 40555823 - Gm35177 NNNTGATGCTGGGAATGACA NGG 2 0.2327 Tier III
36 NC_000075.6 9 40555823 - Gm48277 NNNTGATGCTGGGAATGACA NGG 2 0.2327 Tier III
37 NC_000067.6 1 180208830 + LOC108167750 NNNGTATGTTGGGAATTACA NGG 2 0.2188 Tier III
38 NC_000080.6 14 79113850 + Gm30970 NNNTAATGCTGGGAATGACA NGG 2 0.1818 Tier III
39 NC_000075.6 9 106266812 - Gm33564 NNNGTATGCTGGGCATGCCA NGG 2 0.0408 Tier III
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Other clones with same target sequence:

(none)