Construct: sgRNA BRDN0001145125
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGAAGGTTTGATGTCACGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMKK2 (10645)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76698
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
121253448 |
+ |
CAMKK2 |
NNNAAGGTTTGATGTCACGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
101613381 |
- |
GRIN3A |
NNNAAGTTTTGCTGTCACGG |
NGG |
2 |
0.1504 |
Tier I |
| 3 |
NC_000005.10 |
5 |
94882323 |
- |
MCTP1 |
NNNAAGGATTGATGTCACTG |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000002.12 |
2 |
106094609 |
- |
UXS1 |
NNNAAGGGTTGATGTCACGT |
NGG |
2 |
0.5133 |
Tier II |
| 5 |
NC_000011.10 |
11 |
131575309 |
- |
NTM |
NNNAAGGTTTAATGTGACGG |
NGG |
2 |
0.1538 |
Tier II |
| 6 |
NC_000001.11 |
1 |
63164280 |
- |
LINC00466 |
NNNGAGGTTTGATGTCACAG |
NGG |
2 |
0.4464 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
122746425 |
+ |
Camkk2 |
NNNAAGGTTTGATGTCCCGA |
NGG |
2 |
0.1654 |
Tier I |
| 2 |
NC_000069.6 |
3 |
65297370 |
+ |
Kcnab1 |
NNNAAGATTAGATGTCACGG |
NGG |
2 |
0.8571 |
Tier II |
| 3 |
NC_000068.7 |
2 |
104151476 |
- |
D430041D05Rik |
NNNAAGGCTTGATGTCACAG |
NGG |
2 |
0.7143 |
Tier II |
| 4 |
NC_000086.7 |
X |
7595285 |
- |
Ccdc22 |
NNNAAGGTATGATGTCACTG |
NGG |
2 |
0.619 |
Tier II |
| 5 |
NC_000085.6 |
19 |
50398019 |
- |
Sorcs1 |
NNNAAGGTTAGATGTCAAGG |
NGG |
2 |
0.4615 |
Tier II |
| 6 |
NC_000077.6 |
11 |
63061723 |
+ |
Tekt3 |
NNNAAGGTTTGATGTCAAGT |
NGG |
2 |
0.3769 |
Tier II |
| 7 |
NC_000070.6 |
4 |
98772060 |
+ |
Kank4 |
NNNAAGGTTTGATTTCATGG |
NGG |
2 |
0.1714 |
Tier II |
| 8 |
NC_000075.6 |
9 |
46088998 |
- |
Sik3 |
NNNAAGGTTTGATGTCCAGG |
NGG |
2 |
0.095 |
Tier II |
| 9 |
NC_000081.6 |
15 |
73015423 |
+ |
Trappc9 |
NNNATGGTTTGATGTCACGG |
NTG |
2 |
0.0142 |
Tier II |
| 10 |
NC_000069.6 |
3 |
65297370 |
+ |
A330015K06Rik |
NNNAAGATTAGATGTCACGG |
NGG |
2 |
0.8571 |
Tier III |
| 11 |
NC_000068.7 |
2 |
158084642 |
+ |
2010009K17Rik |
NNNAAGGTTTGAGGTCTCGG |
NGG |
2 |
0.0348 |
Tier III |
Other clones with same target sequence:
(none)
