Construct: sgRNA BRDN0001145137
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGACGTGGAGTGCACTATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCB (5579)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75956
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
24154781 |
+ |
PRKCB |
NNNCGTGGAGTGCACTATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
66735586 |
+ |
PRKCA |
NNNCGTGGAGTGCACCATGG |
NAG |
2 |
0.1728 |
Tier I |
| 3 |
NC_000003.12 |
3 |
53186264 |
+ |
PRKCD |
NNNCGTGGAGTGCACCATGG |
NTG |
2 |
0.026 |
Tier I |
| 4 |
NC_000004.12 |
4 |
859588 |
- |
GAK |
NNNCGTGGAGGGCACTTTGG |
NGG |
2 |
0.0533 |
Tier II |
| 5 |
NC_000003.12 |
3 |
714385 |
- |
LINC01266 |
NNNCCTGGAGTGCACTATGG |
NAG |
2 |
0.2037 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
107983962 |
- |
Prkca |
NNNCGTGGAGTGCACCATGG |
NGG |
1 |
0.6667 |
Tier I |
| 2 |
NC_000073.6 |
7 |
122568231 |
+ |
Prkcb |
NNNTGTGGAGTGCACCATGG |
NGG |
2 |
0.5333 |
Tier I |
| 3 |
NC_000080.6 |
14 |
9810846 |
- |
Fhit |
NNNTGTGGAGTGCACAATGG |
NGG |
2 |
0.7273 |
Tier II |
| 4 |
NC_000075.6 |
9 |
3681779 |
- |
Gucy1a2 |
NNNCGTGGAGTCCACAATGG |
NGG |
2 |
0.4813 |
Tier II |
| 5 |
NC_000072.6 |
6 |
38960144 |
- |
Tbxas1 |
NNNGGTGGAGTCCACTATGG |
NGG |
2 |
0.2647 |
Tier II |
| 6 |
NC_000073.6 |
7 |
145246642 |
+ |
Tpcn2 |
NNNCTTGGAGTGCACTATGG |
NGT |
2 |
0.0048 |
Tier II |
Other clones with same target sequence:
(none)
