Construct: sgRNA BRDN0001145139
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGGGGCGGGATCCACCCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP5KL1 (138429)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75822
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
127925947 |
+ |
PIP5KL1 |
NNNGGGCGGGATCCACCCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
102844637 |
+ |
PAH |
NNNGGGCGGGAACCACCCAA |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000006.12 |
6 |
75994713 |
- |
IMPG1 |
NNNGGGAGGGACCCACCCAG |
NGG |
2 |
0.7105 |
Tier II |
| 4 |
NC_000003.12 |
3 |
54761886 |
- |
CACNA2D3 |
NNNGGTCGGGACCCACCCAG |
NGG |
2 |
0.6316 |
Tier II |
| 5 |
NC_000004.12 |
4 |
9170558 |
- |
FAM90A26 |
NNNAGGCTGGATCCACCCAG |
NGG |
2 |
0.5625 |
Tier II |
| 6 |
NC_000015.10 |
15 |
84955169 |
- |
SLC28A1 |
NNNGGGCTGGAACCACCCAG |
NGG |
2 |
0.5 |
Tier II |
| 7 |
NC_000015.10 |
15 |
75658840 |
- |
SNX33 |
NNNGGGATGGATCCACCCAG |
NGG |
2 |
0.4688 |
Tier II |
| 8 |
NC_000005.10 |
5 |
96692144 |
- |
CAST |
NNNGGGCTGGATCCACCCTG |
NGG |
2 |
0.3365 |
Tier II |
| 9 |
NC_000016.10 |
16 |
85403354 |
+ |
GSE1 |
NNNGGGCGGGGGCCACCCAG |
NGG |
2 |
0.325 |
Tier II |
| 10 |
NC_000006.12 |
6 |
138911979 |
+ |
REPS1 |
NNNGGGAGGCATCCACCCAG |
NGG |
2 |
0.3 |
Tier II |
| 11 |
NC_000019.10 |
19 |
53943112 |
- |
CACNG7 |
NNNGGGCGGGATGCACCCAA |
NGG |
2 |
0.1278 |
Tier II |
| 12 |
NC_000004.12 |
4 |
87189796 |
+ |
KLHL8 |
NNNGGGCGGGATCCACCGAG |
NAG |
2 |
0.0346 |
Tier II |
| 13 |
NC_000011.10 |
11 |
128941373 |
- |
TP53AIP1 |
NNNGGGAGGGATCCACCCAG |
NTG |
2 |
0.0292 |
Tier II |
| 14 |
NC_000007.14 |
7 |
122329382 |
+ |
CADPS2 |
NNNGGGCGGGATCCACGCAG |
NTG |
2 |
0.0023 |
Tier II |
| 15 |
NC_000004.12 |
4 |
3938258 |
+ |
ALG1L7P |
NNNAGGCTGGATCCACCCAG |
NGG |
2 |
0.5625 |
Tier III |
| 16 |
NC_000004.12 |
4 |
9708038 |
- |
ALG1L3P |
NNNAGGCTGGATCCACCCAG |
NGG |
2 |
0.5625 |
Tier III |
| 17 |
NC_000022.11 |
22 |
49627336 |
+ |
MIR3667HG |
NNNGAGGGGGATCCACCCAG |
NGG |
2 |
0.4078 |
Tier III |
| 18 |
NC_000022.11 |
22 |
49627265 |
+ |
MIR3667HG |
NNNGGGGGGGATCCACCCGG |
NGG |
2 |
0.1765 |
Tier III |
| 19 |
NC_000022.11 |
22 |
49627300 |
+ |
MIR3667HG |
NNNGGGGGGGATCCACCCGG |
NGG |
2 |
0.1765 |
Tier III |
| 20 |
NC_000005.10 |
5 |
176661773 |
+ |
LOC124901144 |
NNNGGGTGGGATCCACCCCG |
NGG |
2 |
0.1681 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
32579974 |
- |
Pip5kl1 |
NNNGGGCTGGATCTACCCAG |
NGG |
2 |
0.4583 |
Tier I |
| 2 |
NC_000084.6 |
18 |
80063950 |
+ |
Pard6g |
NNNGGGCAGTATCCACCCAG |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000071.6 |
5 |
14025394 |
- |
Sema3e |
NNNGCGCGGGATCCACCCAG |
NGG |
1 |
0.7857 |
Tier II |
| 4 |
NC_000085.6 |
19 |
57212570 |
- |
Ablim1 |
NNNGGCTGGGATCCACCCAG |
NGG |
2 |
0.554 |
Tier II |
| 5 |
NC_000074.6 |
8 |
84741244 |
+ |
Nfix |
NNNGGGAGGGCTCCACCCAG |
NGG |
2 |
0.3 |
Tier II |
| 6 |
NC_000077.6 |
11 |
109513443 |
- |
Arsg |
NNNGGGCGGGATCCACGGAG |
NGG |
2 |
0.0078 |
Tier II |
| 7 |
NC_000072.6 |
6 |
23273295 |
+ |
Cadps2 |
NNNGGGCGGGATCCACGCAG |
NTG |
2 |
0.0023 |
Tier II |
Other clones with same target sequence:
(none)
