Construct: sgRNA BRDN0001145143
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTATTGGACCTACCAACCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GUCY2F (2986)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77253
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
109475554 |
- |
GUCY2F |
NNNATTGGACCTACCAACCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
115302409 |
- |
LUZP4 |
NNNACTGGACCAACCAACCC |
NGG |
2 |
0.8 |
Tier II |
| 3 |
NC_000002.12 |
2 |
130962476 |
- |
ARHGEF4 |
NNNATTTGACCTAACAACCC |
NGG |
2 |
0.2 |
Tier II |
| 4 |
NC_000016.10 |
16 |
12167541 |
- |
SNX29 |
NNNATTGCACCTCCCAACCC |
NGG |
2 |
0.1296 |
Tier II |
| 5 |
NC_000003.12 |
3 |
126819773 |
+ |
CHCHD6 |
NNNATTGGACCTACAAGCCC |
NGG |
2 |
0.0392 |
Tier II |
| 6 |
NC_000007.14 |
7 |
18680102 |
+ |
HDAC9 |
NNNATGGGACCTACGAACCC |
NGG |
2 |
0.0286 |
Tier II |
| 7 |
NC_000016.10 |
16 |
19745793 |
- |
IQCK |
NNNATTGGACCTTCCAACCC |
NGC |
2 |
0.0067 |
Tier II |
| 8 |
NC_000008.11 |
8 |
138270108 |
- |
FAM135B |
NNNATTGGACCTCCCTACCC |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000016.10 |
16 |
55429645 |
- |
MMP2-AS1 |
NNNTTTGGAGCTACCAACCC |
NGG |
2 |
0.2475 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
84081615 |
+ |
Sult4a1 |
NNNACTGCACCTACCAACCC |
NGG |
2 |
0.6154 |
Tier II |
| 2 |
NC_000069.6 |
3 |
36582063 |
- |
Bbs7 |
NNNATGGGGCCTACCAACCC |
NGG |
2 |
0.381 |
Tier II |
| 3 |
NC_000073.6 |
7 |
123111767 |
- |
Tnrc6a |
NNNATTGGACCTACCAACAC |
NAG |
2 |
0.1111 |
Tier II |
| 4 |
NC_000078.6 |
12 |
51386385 |
+ |
Scfd1 |
NNNATTGGAGCTACCAGCCC |
NGG |
2 |
0.0686 |
Tier II |
| 5 |
NC_000069.6 |
3 |
109968199 |
+ |
Ntng1 |
NNNATTGCACCTACCCACCC |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000078.6 |
12 |
51386385 |
+ |
Gm40422 |
NNNATTGGAGCTACCAGCCC |
NGG |
2 |
0.0686 |
Tier III |
| 7 |
NC_000068.7 |
2 |
94237626 |
- |
Mir670hg |
NNNAATGGACCTACCAAGCC |
NGG |
2 |
0.0667 |
Tier III |
| 8 |
NC_000073.6 |
7 |
39397641 |
+ |
Gm18831 |
NNNATTAGACCTACCAACCC |
NGT |
2 |
0.0161 |
Tier III |
Other clones with same target sequence:
(none)
