Construct: sgRNA BRDN0001145146

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGACATGACCAGCAATAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK36 (27148)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77276

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 218679242 - STK36 NNNCATGACCAGCAATAAAG NGG 0 1.0 Tier I
2 NC_000023.11 X 31729661 - DMD NNNCAAGACCAGCAATCAAG NGG 2 0.1529 Tier I
3 NC_000017.11 17 29896637 - SSH2 NNNAAAGACCAGCAATAAAG NGG 2 0.7298 Tier II
4 NC_000023.11 X 47878655 + ZNF81 NNNAATGACAAGCAATAAAG NGG 2 0.7298 Tier II
5 NC_000001.11 1 183945267 - COLGALT2 NNNCAAGACCAGCAACAAAG NGG 2 0.5778 Tier II
6 NC_000019.10 19 57220172 - ZNF264 NNNCATGAGAAGCAATAAAG NGG 2 0.5365 Tier II
7 NC_000008.11 8 112900851 - CSMD3 NNNCATTAACAGCAATAAAG NGG 2 0.4898 Tier II
8 NC_000002.12 2 55528267 - CFAP36 NNNGATGATCAGCAATAAAG NGG 2 0.4375 Tier II
9 NC_000002.12 2 2105493 - MYT1L NNNCATGAGCAGAAATAAAG NGG 2 0.2381 Tier II
10 NC_000011.10 11 24618078 - LUZP2 NNNCATGCCCAGCAATAATG NGG 2 0.2308 Tier II
11 NC_000011.10 11 103215096 + DYNC2H1 NNNCATGCCCAGCTATAAAG NGG 2 0.2286 Tier II
12 NC_000002.12 2 80498252 - CTNNA2 NNNCATGTCCAGCTATAAAG NGG 2 0.2286 Tier II
13 NC_000003.12 3 189880674 - TP63 NNNCATGACCAGCATTAAAA NGG 2 0.1875 Tier II
14 NC_000001.11 1 36970731 - GRIK3 NNNCATGACCAGCAATGAAG NGG 1 0.1765 Tier II
15 NC_000003.12 3 9247810 + SRGAP3 NNNCATGACCAGAAAGAAAG NGG 2 0.1331 Tier II
16 NC_000008.11 8 78559315 + PKIA NNNCATGATCAGCAATTAAG NGG 2 0.1167 Tier II
17 NC_000007.14 7 147935698 - CNTNAP2 NNNCATGACCAGAATTAAAG NGG 2 0.0769 Tier II
18 NC_000010.11 10 22941902 - ARMC3 NNNCATGACCAGCAATTGAG NGG 2 0.0533 Tier II
19 NC_000003.12 3 107320895 + DUBR NNNTATGACCAACAATAAAG NGG 2 0.7467 Tier III
20 NC_000003.12 3 28018475 + LINC01967 NNNCATGTCCAGCAATAAAA NGG 2 0.4018 Tier III
21 NC_000017.11 17 32428675 - NUDT15P2 NNNCATGACCAGCAACAGAG NGG 2 0.2667 Tier III
22 NC_000001.11 1 209373538 + LINC01698 NNNCATGACCAGCAAGAGAG NGG 2 0.1385 Tier III
23 NC_000008.11 8 78559315 + LOC105375911 NNNCATGATCAGCAATTAAG NGG 2 0.1167 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 33780361 - Sult3a2 NNNCATGAACAACAATAAAG NGG 2 0.8 Tier II
2 NC_000070.6 4 43659634 - Fam221b NNNCAAGATCAGCAATAAAG NGG 2 0.7583 Tier II
3 NC_000070.6 4 145140307 + Vps13d NNNTATGACCAGCAACAAAG NGG 2 0.5333 Tier II
4 NC_000084.6 18 7411887 - Mpp7 NNNCATGACCAGAAATAAAG NGG 1 0.3846 Tier II
5 NC_000074.6 8 88574678 + Nkd1 NNNTATGACCAGCAATAGAG NGG 2 0.32 Tier II
6 NC_000068.7 2 70288835 - Myo3b NNNCATGAACAGCAAGAAAG NGG 2 0.2967 Tier II
7 NC_000074.6 8 45313378 + Cyp4v3 NNNCATGACCATCTATAAAG NGG 2 0.2051 Tier II
8 NC_000086.7 X 137927358 - Il1rapl2 NNNCATGACAAGGAATAAAG NGG 2 0.1182 Tier II
9 NC_000071.6 5 107345971 - Brdt NNNCATGACCAGCGATAAAG NGT 2 0.0075 Tier II
10 NC_000086.7 X 7295530 + Clcn5 NNNCATGCCCAGCAATAAAG NGT 2 0.0069 Tier II
11 NC_000082.6 16 6157405 - Rbfox1 NNNCATGACCAGCAATAAAG NAC 2 0.0 Tier II
12 NC_000070.6 4 43659634 - Gm4748 NNNCAAGATCAGCAATAAAG NGG 2 0.7583 Tier III
13 NC_000074.6 8 45313378 + Gm30504 NNNCATGACCATCTATAAAG NGG 2 0.2051 Tier III
14 NC_000071.6 5 43180325 + Gm7854 NNNCATGACCAGCAAAACAG NGG 2 0.1732 Tier III
15 NC_000078.6 12 32932619 + 4933406C10Rik NNNCCTGACCAGCAAGAAAG NGG 2 0.1731 Tier III
16 NC_000074.6 8 103297624 + Gm39234 NNNCATGAGCAGCCATAAAG NGG 2 0.1327 Tier III
17 NC_000068.7 2 132642874 - AU019990 NNNCATGACCACCAATGAAG NGG 2 0.0934 Tier III
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Other clones with same target sequence:

(none)