Construct: sgRNA BRDN0001145147
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGTTGCAGGTGATAACGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK32A (202374)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76475
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
147323964 |
- |
STK32A |
NNNGTTGCAGGTGATAACGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
85328633 |
- |
CA1 |
NNNGTTGAAGGTGATAACGC |
NGT |
2 |
0.0105 |
Tier I |
| 3 |
NC_000018.10 |
18 |
23200938 |
- |
CABLES1 |
NNNGCTGCAGGAGATAACGC |
NGG |
2 |
0.8 |
Tier II |
| 4 |
NC_000002.12 |
2 |
219420053 |
- |
DES |
NNNGTTGCGGGTGATAAAGC |
NGG |
2 |
0.359 |
Tier II |
| 5 |
NC_000001.11 |
1 |
230361217 |
+ |
PGBD5 |
NNNGTTGCGGGGGATAACGC |
NGG |
2 |
0.3333 |
Tier II |
| 6 |
NC_000019.10 |
19 |
38291029 |
+ |
SPINT2 |
NNNGTTGCAGGTGATAATGT |
NGG |
2 |
0.1929 |
Tier II |
| 7 |
NC_000002.12 |
2 |
40140192 |
- |
SLC8A1 |
NNNGTTGCAGGAGATGACGC |
NGG |
2 |
0.1538 |
Tier II |
| 8 |
NC_000022.11 |
22 |
40081599 |
- |
TNRC6B |
NNNGTTGCAGGTGACAAAGC |
NGG |
2 |
0.1469 |
Tier II |
| 9 |
NC_000003.12 |
3 |
32863529 |
- |
TRIM71 |
NNNGTTGCAGGTGAGAACGA |
NGG |
2 |
0.025 |
Tier II |
| 10 |
NC_000020.11 |
20 |
52330881 |
- |
LOC105372666 |
NNNTTTGCAGGTGATAAAGC |
NGG |
2 |
0.1958 |
Tier III |
| 11 |
NC_000002.12 |
2 |
40140192 |
- |
SLC8A1-AS1 |
NNNGTTGCAGGAGATGACGC |
NGG |
2 |
0.1538 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
43261314 |
- |
Stk32a |
NNNGCTGCAGGTGATAACGC |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000078.6 |
12 |
89411722 |
+ |
Nrxn3 |
NNNGTTGCAGTTGATAACCC |
NGG |
2 |
0.1724 |
Tier II |
| 3 |
NC_000068.7 |
2 |
27402375 |
+ |
Vav2 |
NNNGTTGCAGGTGATAGCAC |
NGG |
2 |
0.1261 |
Tier II |
| 4 |
NC_000067.6 |
1 |
131260337 |
+ |
Ikbke |
NNNGTTGCAGGTGGGAACGC |
NGG |
2 |
0.0233 |
Tier II |
| 5 |
NC_000081.6 |
15 |
7314917 |
- |
Egflam |
NNNGTTGCAGGTTAGAACGC |
NGG |
2 |
0.015 |
Tier II |
| 6 |
NC_000079.6 |
13 |
114588160 |
- |
4930467J12Rik |
NNNGTTGCAGTTGATAACCC |
NGG |
2 |
0.1724 |
Tier III |
| 7 |
NC_000067.6 |
1 |
131260337 |
+ |
Gm28913 |
NNNGTTGCAGGTGGGAACGC |
NGG |
2 |
0.0233 |
Tier III |
Other clones with same target sequence:
(none)
