Construct: sgRNA BRDN0001145152
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAGACAGGTTGCTCAAAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TTK (7272)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75579
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000006.12 |
6 |
80026394 |
- |
TTK |
NNNGACAGGTTGCTCAAAAG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000014.9 |
14 |
100550822 |
- |
BEGAIN |
NNNGACAGGATGCTCAAAAG |
NGG |
1 |
0.8571 |
Tier II |
3 |
NC_000010.11 |
10 |
76914584 |
+ |
KCNMA1 |
NNNGACAGAATGCTCAAAAG |
NGG |
2 |
0.551 |
Tier II |
4 |
NC_000010.11 |
10 |
61709234 |
+ |
CABCOCO1 |
NNNGACAGGTTCCTCAAAAG |
NGG |
1 |
0.5294 |
Tier II |
5 |
NC_000002.12 |
2 |
106508673 |
- |
CD8B2 |
NNNGACAGGTACCTCAAAAG |
NGG |
2 |
0.3971 |
Tier II |
6 |
NC_000002.12 |
2 |
7065412 |
+ |
RNF144A |
NNNGGCAGGTTCCTCAAAAG |
NGG |
2 |
0.3812 |
Tier II |
7 |
NC_000002.12 |
2 |
191071116 |
- |
STAT4 |
NNNCACAGGTTGCTCAAAAT |
NGG |
2 |
0.3706 |
Tier II |
8 |
NC_000007.14 |
7 |
131519597 |
+ |
PODXL |
NNNGAGAGGTTGATCAAAAG |
NGG |
2 |
0.1923 |
Tier II |
9 |
NC_000016.10 |
16 |
10150250 |
- |
GRIN2A |
NNNGAGAGGTTGATCAAAAG |
NGG |
2 |
0.1923 |
Tier II |
10 |
NC_000004.12 |
4 |
86662656 |
- |
PTPN13 |
NNNGACAGTTTGCTTAAAAG |
NGG |
2 |
0.0356 |
Tier II |
11 |
NC_000001.11 |
1 |
40985637 |
+ |
CTPS1 |
NNNGAGAGGTTGCTGAAAAG |
NGG |
2 |
0.025 |
Tier II |
12 |
NC_000010.11 |
10 |
76914584 |
+ |
KCNMA1-AS1 |
NNNGACAGAATGCTCAAAAG |
NGG |
2 |
0.551 |
Tier III |
13 |
NC_000010.11 |
10 |
4763731 |
- |
LOC105376374 |
NNNGGCAGATTGCTCAAAAG |
NGG |
2 |
0.4629 |
Tier III |
14 |
NC_000010.11 |
10 |
4763731 |
- |
LOC105376375 |
NNNGGCAGATTGCTCAAAAG |
NGG |
2 |
0.4629 |
Tier III |
15 |
NC_000002.12 |
2 |
7065412 |
+ |
LOC101929452 |
NNNGGCAGGTTCCTCAAAAG |
NGG |
2 |
0.3812 |
Tier III |
16 |
NC_000002.12 |
2 |
69453518 |
+ |
LOC124906020 |
NNNGAGAGGTTGTTCAAAAG |
NGG |
2 |
0.35 |
Tier III |
17 |
NC_000005.10 |
5 |
173479855 |
+ |
LOC105377732 |
NNNGCCAGGTTGCTCAATAG |
NGG |
2 |
0.25 |
Tier III |
18 |
NC_000015.10 |
15 |
25207276 |
- |
SNHG14 |
NNNGACAGGTTGCTCAATAG |
NAG |
2 |
0.1296 |
Tier III |
19 |
NC_000001.11 |
1 |
198864148 |
+ |
MIR181A1HG |
NNNGACAGGTAGCTCTAAAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000076.6 |
10 |
116341511 |
- |
Ptprb |
NNNGACACGTTGCTCAAAAG |
NAG |
2 |
0.1595 |
Tier I |
2 |
NC_000069.6 |
3 |
45380001 |
- |
Pcdh10 |
NNNGACGGGTTGGTCAAAAG |
NGG |
2 |
0.0963 |
Tier I |
3 |
NC_000072.6 |
6 |
90107574 |
- |
Vmn1r50 |
NNNGACAGGTTGCACAAAAG |
NTG |
2 |
0.0241 |
Tier I |
4 |
NC_000086.7 |
X |
169292606 |
- |
Arhgap6 |
NNNGACAAGTTGTTCAAAAG |
NGG |
2 |
0.7 |
Tier II |
5 |
NC_000070.6 |
4 |
96777435 |
+ |
Gm12695 |
NNNAACAGGTTGTTCAAAAG |
NGG |
2 |
0.63 |
Tier II |
6 |
NC_000072.6 |
6 |
64361809 |
- |
Grid2 |
NNNAACAGTTTGCTCAAAAG |
NGG |
2 |
0.48 |
Tier II |
7 |
NC_000080.6 |
14 |
14905024 |
+ |
Nek10 |
NNNGGCAGCTTGCTCAAAAG |
NGG |
2 |
0.3877 |
Tier II |
8 |
NC_000078.6 |
12 |
55623913 |
+ |
Ralgapa1 |
NNNGACAGCTTGCTCAGAAG |
NGG |
2 |
0.095 |
Tier II |
9 |
NC_000079.6 |
13 |
99996567 |
+ |
Mccc2 |
NNNGACAGGGTGCTCACAAG |
NGG |
2 |
0.0882 |
Tier II |
10 |
NC_000075.6 |
9 |
88810046 |
- |
Gm10634 |
NNNGACAGGTTGATCAAAAG |
NGT |
2 |
0.0062 |
Tier II |
11 |
NC_000072.6 |
6 |
9146969 |
+ |
Nxph1 |
NNNGACAGGTTGGTCAAAAG |
NTG |
2 |
0.0053 |
Tier II |
12 |
NC_000070.6 |
4 |
145261329 |
- |
Gm36233 |
NNNGACAGGATGCTCAGAAG |
NGG |
2 |
0.1513 |
Tier III |
13 |
NC_000075.6 |
9 |
89120443 |
+ |
4930579C12Rik |
NNNGACAGGTTGATCAAAAG |
NGT |
2 |
0.0062 |
Tier III |
Other clones with same target sequence:
(none)