Construct: sgRNA BRDN0001145152

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAAGACAGGTTGCTCAAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTK (7272)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75579

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 80026394 - TTK NNNGACAGGTTGCTCAAAAG NGG 0 1.0 Tier I
2 NC_000014.9 14 100550822 - BEGAIN NNNGACAGGATGCTCAAAAG NGG 1 0.8571 Tier II
3 NC_000010.11 10 76914584 + KCNMA1 NNNGACAGAATGCTCAAAAG NGG 2 0.551 Tier II
4 NC_000010.11 10 61709234 + CABCOCO1 NNNGACAGGTTCCTCAAAAG NGG 1 0.5294 Tier II
5 NC_000002.12 2 106508673 - CD8B2 NNNGACAGGTACCTCAAAAG NGG 2 0.3971 Tier II
6 NC_000002.12 2 7065412 + RNF144A NNNGGCAGGTTCCTCAAAAG NGG 2 0.3812 Tier II
7 NC_000002.12 2 191071116 - STAT4 NNNCACAGGTTGCTCAAAAT NGG 2 0.3706 Tier II
8 NC_000007.14 7 131519597 + PODXL NNNGAGAGGTTGATCAAAAG NGG 2 0.1923 Tier II
9 NC_000016.10 16 10150250 - GRIN2A NNNGAGAGGTTGATCAAAAG NGG 2 0.1923 Tier II
10 NC_000004.12 4 86662656 - PTPN13 NNNGACAGTTTGCTTAAAAG NGG 2 0.0356 Tier II
11 NC_000001.11 1 40985637 + CTPS1 NNNGAGAGGTTGCTGAAAAG NGG 2 0.025 Tier II
12 NC_000010.11 10 76914584 + KCNMA1-AS1 NNNGACAGAATGCTCAAAAG NGG 2 0.551 Tier III
13 NC_000010.11 10 4763731 - LOC105376374 NNNGGCAGATTGCTCAAAAG NGG 2 0.4629 Tier III
14 NC_000010.11 10 4763731 - LOC105376375 NNNGGCAGATTGCTCAAAAG NGG 2 0.4629 Tier III
15 NC_000002.12 2 7065412 + LOC101929452 NNNGGCAGGTTCCTCAAAAG NGG 2 0.3812 Tier III
16 NC_000002.12 2 69453518 + LOC124906020 NNNGAGAGGTTGTTCAAAAG NGG 2 0.35 Tier III
17 NC_000005.10 5 173479855 + LOC105377732 NNNGCCAGGTTGCTCAATAG NGG 2 0.25 Tier III
18 NC_000015.10 15 25207276 - SNHG14 NNNGACAGGTTGCTCAATAG NAG 2 0.1296 Tier III
19 NC_000001.11 1 198864148 + MIR181A1HG NNNGACAGGTAGCTCTAAAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 116341511 - Ptprb NNNGACACGTTGCTCAAAAG NAG 2 0.1595 Tier I
2 NC_000069.6 3 45380001 - Pcdh10 NNNGACGGGTTGGTCAAAAG NGG 2 0.0963 Tier I
3 NC_000072.6 6 90107574 - Vmn1r50 NNNGACAGGTTGCACAAAAG NTG 2 0.0241 Tier I
4 NC_000086.7 X 169292606 - Arhgap6 NNNGACAAGTTGTTCAAAAG NGG 2 0.7 Tier II
5 NC_000070.6 4 96777435 + Gm12695 NNNAACAGGTTGTTCAAAAG NGG 2 0.63 Tier II
6 NC_000072.6 6 64361809 - Grid2 NNNAACAGTTTGCTCAAAAG NGG 2 0.48 Tier II
7 NC_000080.6 14 14905024 + Nek10 NNNGGCAGCTTGCTCAAAAG NGG 2 0.3877 Tier II
8 NC_000078.6 12 55623913 + Ralgapa1 NNNGACAGCTTGCTCAGAAG NGG 2 0.095 Tier II
9 NC_000079.6 13 99996567 + Mccc2 NNNGACAGGGTGCTCACAAG NGG 2 0.0882 Tier II
10 NC_000075.6 9 88810046 - Gm10634 NNNGACAGGTTGATCAAAAG NGT 2 0.0062 Tier II
11 NC_000072.6 6 9146969 + Nxph1 NNNGACAGGTTGGTCAAAAG NTG 2 0.0053 Tier II
12 NC_000070.6 4 145261329 - Gm36233 NNNGACAGGATGCTCAGAAG NGG 2 0.1513 Tier III
13 NC_000075.6 9 89120443 + 4930579C12Rik NNNGACAGGTTGATCAAAAG NGT 2 0.0062 Tier III
Download CSV

Other clones with same target sequence:

(none)