Construct: sgRNA BRDN0001145157

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACAAAGCGATTTAAAGCGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HK1 (3098)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77643

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 69368571 + HK1 NNNAAGCGATTTAAAGCGAG NGG 0 1.0 Tier I
2 NC_000010.11 10 93349990 + MYOF NNNAAACGATTTAAAGAGAG NGG 2 0.4667 Tier II
3 NC_000002.12 2 40023818 - SLC8A1-AS1 NNNTAGCGATTTAAAGCAAG NGG 2 0.4406 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 112249994 + 2900079G21Rik NNNAAGCTATTTAAAGGGAG NGG 2 0.0368 Tier III
Download CSV

Other clones with same target sequence:

(none)