Construct: sgRNA BRDN0001145161
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGACTACATTAATGGTGGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SGK1 (6446)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77460
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
134173028 |
- |
SGK1 |
NNNCTACATTAATGGTGGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
154954405 |
- |
PAXIP1 |
NNNCTACATTCTTGGTGGAG |
NGG |
2 |
0.1333 |
Tier I |
| 3 |
NC_000002.12 |
2 |
119786755 |
+ |
PTPN4 |
NNNCCACATTAATGGTGGAA |
NGG |
2 |
0.9375 |
Tier II |
| 4 |
NC_000006.12 |
6 |
135479987 |
- |
AHI1 |
NNNCTACAATAATGGTGGAA |
NGG |
2 |
0.8705 |
Tier II |
| 5 |
NC_000005.10 |
5 |
153526168 |
+ |
GRIA1 |
NNNTTACATTAATGATGGAG |
NGG |
2 |
0.7529 |
Tier II |
| 6 |
NC_000003.12 |
3 |
45707657 |
+ |
SACM1L |
NNNCGACATTAATGATGGAG |
NGG |
2 |
0.6024 |
Tier II |
| 7 |
NC_000004.12 |
4 |
151575087 |
- |
FHIP1A |
NNNATACATTGATGGTGGAG |
NGG |
2 |
0.5474 |
Tier II |
| 8 |
NC_000004.12 |
4 |
24391551 |
+ |
PPARGC1A |
NNNCTACAATAATGGTGGTG |
NGG |
2 |
0.5 |
Tier II |
| 9 |
NC_000017.11 |
17 |
49636407 |
- |
SPOP |
NNNTTACAGTAATGGTGGAG |
NGG |
2 |
0.4952 |
Tier II |
| 10 |
NC_000002.12 |
2 |
86140646 |
+ |
PTCD3 |
NNNCAACATTAATGGTAGAG |
NGG |
2 |
0.4667 |
Tier II |
| 11 |
NC_000002.12 |
2 |
208482204 |
- |
PTH2R |
NNNCTACCTAAATGGTGGAG |
NGG |
2 |
0.3673 |
Tier II |
| 12 |
NC_000002.12 |
2 |
117999305 |
+ |
CCDC93 |
NNNCTGCATGAATGGTGGAG |
NGG |
2 |
0.3571 |
Tier II |
| 13 |
NC_000007.14 |
7 |
140857663 |
+ |
BRAF |
NNNCTACATTAATGGGGGAA |
NGG |
2 |
0.3245 |
Tier II |
| 14 |
NC_000006.12 |
6 |
108512489 |
+ |
AFG1L |
NNNCTACAGTAATGGTGGGG |
NGG |
2 |
0.2321 |
Tier II |
| 15 |
NC_000004.12 |
4 |
153243137 |
+ |
TRIM2 |
NNNCTTCATTACTGGTGGAG |
NGG |
2 |
0.188 |
Tier II |
| 16 |
NC_000015.10 |
15 |
25806870 |
- |
ATP10A |
NNNCAACATTTATGGTGGAG |
NGG |
2 |
0.1538 |
Tier II |
| 17 |
NC_000013.11 |
13 |
108517569 |
- |
MYO16 |
NNNCTACATTAATTGTGGTG |
NGG |
2 |
0.1436 |
Tier II |
| 18 |
NC_000007.14 |
7 |
24304789 |
+ |
LOC107986777 |
NNNCAGCATTAATGGTGGAG |
NGG |
2 |
0.3571 |
Tier III |
| 19 |
NC_000005.10 |
5 |
15454479 |
- |
LOC124900945 |
NNNGTCCATTAATGGTGGAG |
NGG |
2 |
0.2273 |
Tier III |
| 20 |
NC_000012.12 |
12 |
118912501 |
- |
LOC105370019 |
NNNCTACAGTAATGGTTGAG |
NGG |
2 |
0.1548 |
Tier III |
| 21 |
NC_000011.10 |
11 |
82472231 |
+ |
LOC105369410 |
NNNCTACATTAATTTTGGAG |
NGG |
2 |
0.0381 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
21996350 |
+ |
Sgk1 |
NNNCTACATTAATGGTGGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000075.6 |
9 |
28347119 |
- |
Opcml |
NNNCTACATTAACTGTGGAG |
NGG |
2 |
0.2105 |
Tier II |
| 3 |
NC_000078.6 |
12 |
50428080 |
+ |
Prkd1 |
NNNCAACATTAATTGTGGAG |
NGG |
2 |
0.1333 |
Tier II |
| 4 |
NC_000077.6 |
11 |
71203072 |
- |
Nlrp1b |
NNNCTACATTAATGGAGGAG |
NGA |
2 |
0.0631 |
Tier II |
| 5 |
NC_000085.6 |
19 |
13012573 |
- |
Gm19132 |
NNNCAATATTAATGGTGGAG |
NGG |
2 |
0.4063 |
Tier III |
Other clones with same target sequence:
(none)
