Construct: sgRNA BRDN0001145164
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGTTGGACAGCTCGATCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP5K1C (23396)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77667
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
3656534 |
+ |
PIP5K1C |
NNNTTGGACAGCTCGATCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
28239692 |
+ |
PAN3 |
NNNTTGGACAGCTCTATGAG |
NGG |
2 |
0.019 |
Tier I |
| 3 |
NC_000008.11 |
8 |
62830674 |
+ |
NKAIN3 |
NNNTTGGACAGCTCAATCAG |
NAG |
2 |
0.244 |
Tier II |
| 4 |
NC_000012.12 |
12 |
119088281 |
+ |
SRRM4 |
NNNTTGGACAGCTTCATCAG |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000005.10 |
5 |
31976185 |
- |
PDZD2 |
NNNTTGGACAGCTCCATAAG |
NGG |
2 |
0.1469 |
Tier II |
| 6 |
NC_000009.12 |
9 |
17477424 |
- |
CNTLN |
NNNTTGGCCAGCTCCATCAG |
NGG |
2 |
0.1169 |
Tier II |
| 7 |
NC_000017.11 |
17 |
28968108 |
+ |
SEZ6 |
NNNTTGGACAGCTCGAGCAC |
NGG |
2 |
0.0504 |
Tier II |
| 8 |
NC_000012.12 |
12 |
25644070 |
+ |
LMNTD1 |
NNNTTGGACAGCTGGATCAG |
NTG |
2 |
0.0 |
Tier II |
| 9 |
NC_000003.12 |
3 |
196209646 |
- |
ZDHHC19 |
NNNTTGGACAGCTTGCTCAG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000019.10 |
19 |
46372011 |
+ |
PPP5C |
NNNTTGGGCAGCTCGTTCAG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000017.11 |
17 |
28968108 |
+ |
LOC105371716 |
NNNTTGGACAGCTCGAGCAC |
NGG |
2 |
0.0504 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
81307508 |
- |
Pip5k1c |
NNNTTGGAGAGCTCGATGAG |
NGG |
2 |
0.0825 |
Tier I |
| 2 |
NC_000085.6 |
19 |
24381817 |
+ |
Pip5k1b |
NNNTTGGACAGTTCTATCAG |
NGG |
2 |
0.0769 |
Tier I |
| 3 |
NC_000071.6 |
5 |
147503198 |
+ |
Pan3 |
NNNTTGGACAGCTCTATGAG |
NGG |
2 |
0.019 |
Tier I |
| 4 |
NC_000074.6 |
8 |
76907234 |
+ |
Nr3c2 |
NNNTTGGACAACTCGAACAG |
NGG |
2 |
0.5333 |
Tier II |
| 5 |
NC_000083.6 |
17 |
57057805 |
- |
Slc25a23 |
NNNTTGGAGAGCTAGATCAG |
NGG |
2 |
0.2167 |
Tier II |
| 6 |
NC_000076.6 |
10 |
5535244 |
- |
Syne1 |
NNNTTGGACAGCTCTATCAG |
NCG |
2 |
0.0153 |
Tier II |
Other clones with same target sequence:
(none)
