Construct: sgRNA BRDN0001145169

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTCGGACCACATATTCAGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MST1R (4486)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78052

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 49896364 + MST1R NNNGGACCACATATTCAGGA NGG 0 1.0 Tier I
2 NC_000010.11 10 35148732 - CREM NNNGGATTACATATTCAGGA NGG 2 0.7109 Tier II
3 NC_000006.12 6 12081076 - HIVEP1 NNNGGACCAAATATACAGGA NGG 2 0.5018 Tier II
4 NC_000017.11 17 36493675 + ZNHIT3 NNNGGACCACATATACAGTA NGG 2 0.386 Tier II
5 NC_000004.12 4 7253328 - SORCS2 NNNGGGCCACAGATTCAGGA NGG 2 0.3571 Tier II
6 NC_000007.14 7 129871034 - UBE2H NNNGGATCAGATATTCAGGA NGG 2 0.316 Tier II
7 NC_000020.11 20 49454557 + KCNB1 NNNGGAGCACATATTCAGGT NGG 2 0.2824 Tier II
8 NC_000017.11 17 32670942 + MYO1D NNNGGACCAGATATTCAGAA NGG 2 0.2778 Tier II
9 NC_000019.10 19 9533686 + ZNF426 NNNGGACCACATTTTCAGAA NGG 2 0.2143 Tier II
10 NC_000017.11 17 2075304 + SMG6 NNNGGACTACATCTTCAGGA NGG 2 0.1842 Tier II
11 NC_000007.14 7 158213292 + PTPRN2 NNNGAACCACATATTCTGGA NGG 2 0.1156 Tier II
12 NC_000017.11 17 57427562 - MSI2 NNNGGACCACATAATGAGGA NGG 2 0.0952 Tier II
13 NC_000015.10 15 85047764 + PDE8A NNNAGACCACATATTCAGGA NTG 2 0.0351 Tier II
14 NC_000011.10 11 89787257 + TRIM49 NNNGGACCACATAGTCAGGA NGA 2 0.0 Tier II
15 NC_000011.10 11 90052421 - TRIM49C NNNGGACCACATAGTCAGGA NGA 2 0.0 Tier II
16 NC_000011.10 11 49048852 - ANKRD33BP3 NNNGGACCACATAGTCAGGA NGA 2 0.0 Tier III
17 NC_000011.10 11 89787257 + ANKRD33BP7 NNNGGACCACATAGTCAGGA NGA 2 0.0 Tier III
18 NC_000011.10 11 90052421 - ANKRD33BP10 NNNGGACCACATAGTCAGGA NGA 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 80374122 + 5730455P16Rik NNNAGACCACATATTCAGAA NGG 2 0.6429 Tier I
2 NC_000073.6 7 107934198 - Olfr473 NNNGGAGCGCATATTCAGGA NGG 2 0.3137 Tier I
3 NC_000073.6 7 107991043 - Olfr477 NNNGGAGCGCATATTCAGGA NGG 2 0.3137 Tier I
4 NC_000079.6 13 73511326 - Lpcat1 NNNCGACCACATATTCACGA NGG 2 0.2521 Tier I
5 NC_000075.6 9 107914759 - Mst1r NNNGGACCACATATTCAGGC NGG 1 0.2273 Tier I
6 NC_000069.6 3 103836156 - Bcl2l15 NNNGGACCACATATTCAAGA NGT 2 0.0112 Tier I
7 NC_000067.6 1 158290496 + Brinp2 NNNGAACCACACATTCAGGA NGG 2 0.8211 Tier II
8 NC_000082.6 16 6004188 + Rbfox1 NNNGGATCACAAATTCAGGA NGG 2 0.65 Tier II
9 NC_000069.6 3 35974345 + Mccc1 NNNGGACAACACATTCAGGA NGG 2 0.6158 Tier II
10 NC_000083.6 17 52745671 + Kcnh8 NNNGGAACACATATTCAGTA NGG 2 0.5 Tier II
11 NC_000081.6 15 91534211 + Slc2a13 NNNGGACAACATAATCAGGA NGG 2 0.4024 Tier II
12 NC_000071.6 5 62812316 + Dthd1 NNNGGACCACATTTTAAGGA NGG 2 0.3 Tier II
13 NC_000074.6 8 78584564 + Slc10a7 NNNGGACCACAGATACAGGA NGG 2 0.2895 Tier II
14 NC_000067.6 1 37400976 + Inpp4a NNNGGGCCACATATTTAGGA NGG 2 0.2198 Tier II
15 NC_000073.6 7 96384840 + Tenm4 NNNGGACCACATATTCATGC NGG 2 0.1515 Tier II
16 NC_000067.6 1 193235910 + Hsd11b1 NNNGGACCACACATTGAGGA NGG 2 0.1457 Tier II
17 NC_000068.7 2 119542134 + Exd1 NNNGGACCACAGCTTCAGGA NGG 2 0.1053 Tier II
18 NC_000082.6 16 96317095 - B3galt5 NNNGGACCACAGATTCAGGA NGA 2 0.0347 Tier II
19 NC_000067.6 1 9191805 - Sntg1 NNNGGACCACATATCCAGGA NTG 2 0.0106 Tier II
20 NC_000071.6 5 50155419 - 4930448I18Rik NNNGGGCCACATATGCAGGA NGG 2 0.0357 Tier III
Download CSV

Other clones with same target sequence:

(none)