Construct: sgRNA BRDN0001145171
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTAAATACTCACGGATACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GALK2 (2585)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76767
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
49217309 |
- |
GALK2 |
NNNAATACTCACGGATACAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
182689314 |
- |
TENM3 |
NNNAATACTTACGGATACAT |
NGG |
2 |
0.5647 |
Tier II |
| 3 |
NC_000006.12 |
6 |
47539010 |
- |
CD2AP |
NNNACTACTCACAGATACAA |
NGG |
2 |
0.4615 |
Tier II |
| 4 |
NC_000010.11 |
10 |
69478708 |
+ |
TSPAN15 |
NNNAATACACACGGATACAA |
NAG |
2 |
0.2407 |
Tier II |
| 5 |
NC_000014.9 |
14 |
58136824 |
+ |
ARMH4 |
NNNAATATTCACGGATGCAA |
NGG |
2 |
0.1544 |
Tier II |
| 6 |
NC_000003.12 |
3 |
41623215 |
+ |
ULK4 |
NNNAATGCTCACGGATAGAA |
NGG |
2 |
0.0941 |
Tier II |
| 7 |
NC_000004.12 |
4 |
77599216 |
- |
CXCL13 |
NNNAATCCTCACGGATACCA |
NGG |
2 |
0.0905 |
Tier II |
| 8 |
NC_000017.11 |
17 |
66444147 |
+ |
PRKCA |
NNNAATACTCATGGATTCAA |
NGG |
2 |
0.0718 |
Tier II |
| 9 |
NC_000016.10 |
16 |
63518696 |
- |
LOC105371308 |
NNNAATACTCAAAGATACAA |
NGG |
2 |
0.6593 |
Tier III |
| 10 |
NC_000006.12 |
6 |
8241918 |
- |
LOC105374910 |
NNNAATACTCAAGAATACAA |
NGG |
2 |
0.5357 |
Tier III |
| 11 |
NC_000002.12 |
2 |
142300994 |
- |
LOC105373651 |
NNNAATACTCACGGATAGAA |
NGA |
2 |
0.0093 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
125887983 |
- |
Galk2 |
NNNAATACTCACGGATACAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000083.6 |
17 |
48411323 |
- |
Oard1 |
NNNAATCCTCACTGATACAA |
NGG |
2 |
0.1313 |
Tier I |
| 3 |
NC_000071.6 |
5 |
14143438 |
- |
Sema3e |
NNNAATACCCACAGATACAA |
NGG |
2 |
0.8521 |
Tier II |
| 4 |
NC_000068.7 |
2 |
23399374 |
+ |
Nxph2 |
NNNAATACTCACAAATACAA |
NGG |
2 |
0.6923 |
Tier II |
| 5 |
NC_000077.6 |
11 |
81473506 |
+ |
Asic2 |
NNNAAAACTCACGAATACAA |
NGG |
2 |
0.65 |
Tier II |
| 6 |
NC_000070.6 |
4 |
47264945 |
- |
Col15a1 |
NNNAAAACTCAAGGATACAA |
NGG |
2 |
0.619 |
Tier II |
| 7 |
NC_000072.6 |
6 |
90488123 |
+ |
Klf15 |
NNNAATACACATGGATACAA |
NGG |
2 |
0.5 |
Tier II |
| 8 |
NC_000071.6 |
5 |
81128476 |
- |
Adgrl3 |
NNNGATGCTCACGGATACAA |
NGG |
2 |
0.4412 |
Tier II |
| 9 |
NC_000068.7 |
2 |
77019363 |
- |
Ccdc141 |
NNNAATACTCATGAATACAA |
NGG |
2 |
0.4038 |
Tier II |
| 10 |
NC_000074.6 |
8 |
22881373 |
+ |
Kat6a |
NNNAATACACACTGATACAA |
NGG |
2 |
0.2786 |
Tier II |
| 11 |
NC_000085.6 |
19 |
5767796 |
+ |
Scyl1 |
NNNAATACTCAGGGAGACAA |
NGG |
2 |
0.1538 |
Tier II |
| 12 |
NC_000078.6 |
12 |
98517614 |
- |
Kcnk10 |
NNNAATACTCACGGACACAA |
NGA |
2 |
0.0463 |
Tier II |
| 13 |
NC_000077.6 |
11 |
65102605 |
- |
Arhgap44 |
NNNAATACTCATGGATACAA |
NTG |
2 |
0.021 |
Tier II |
| 14 |
NC_000072.6 |
6 |
139803012 |
+ |
Pik3c2g |
NNNAATACTCACGTATACAA |
NTG |
2 |
0.0104 |
Tier II |
| 15 |
NC_000070.6 |
4 |
47264945 |
- |
Gm52734 |
NNNAAAACTCAAGGATACAA |
NGG |
2 |
0.619 |
Tier III |
| 16 |
NC_000080.6 |
14 |
54170657 |
+ |
Tcra |
NNNCATACTCACAGATACAA |
NGG |
2 |
0.3258 |
Tier III |
| 17 |
NC_000077.6 |
11 |
32806023 |
- |
Gm35723 |
NNNAATACTCACGGGTACAA |
NAG |
2 |
0.1685 |
Tier III |
Other clones with same target sequence:
(none)
