Construct: sgRNA BRDN0001145175
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTTGTATGCCCAATCCAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- C17orf75 (64149)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78077
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
32335373 |
- |
C17orf75 |
NNNTGTATGCCCAATCCAAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
39359135 |
+ |
SPATA31A1 |
NNNTGTCTGCCCAATTCAAA |
NGG |
2 |
0.1346 |
Tier I |
| 3 |
NC_000009.12 |
9 |
42187072 |
+ |
SPATA31A6 |
NNNTGTCTGCCCAATTCAAA |
NGG |
2 |
0.1346 |
Tier I |
| 4 |
NC_000009.12 |
9 |
60917826 |
+ |
SPATA31A5 |
NNNTGTCTGCCCAATTCAAA |
NGG |
2 |
0.1346 |
Tier I |
| 5 |
NC_000009.12 |
9 |
61193456 |
+ |
SPATA31A7 |
NNNTGTCTGCCCAATTCAAA |
NGG |
2 |
0.1346 |
Tier I |
| 6 |
NC_000009.12 |
9 |
66989129 |
- |
SPATA31A3 |
NNNTGTCTGCCCAATTCAAA |
NGG |
2 |
0.1346 |
Tier I |
| 7 |
NC_000005.10 |
5 |
148438580 |
- |
FBXO38 |
NNNTGAATGCCAAATCCAAA |
NGG |
2 |
0.619 |
Tier II |
| 8 |
NC_000008.11 |
8 |
101594993 |
- |
GRHL2 |
NNNTGTATGCCCAAAACAAA |
NGG |
2 |
0.5789 |
Tier II |
| 9 |
NC_000005.10 |
5 |
146925574 |
- |
PPP2R2B |
NNNTGTATGCCAAATCCAAA |
NAG |
2 |
0.1852 |
Tier II |
| 10 |
NC_000002.12 |
2 |
36544305 |
- |
CRIM1 |
NNNTGTATTCTCAATCCAAA |
NGG |
2 |
0.1641 |
Tier II |
| 11 |
NC_000008.11 |
8 |
32558051 |
+ |
NRG1 |
NNNTTTATGCCCCATCCAAA |
NGG |
2 |
0.0632 |
Tier II |
| 12 |
NC_000007.14 |
7 |
13985769 |
+ |
ETV1 |
NNNTGTATGCCAAATCCAAA |
NGA |
2 |
0.0496 |
Tier II |
| 13 |
NC_000004.12 |
4 |
54279086 |
+ |
PDGFRA |
NNNTGTATGCCCAAGCCACA |
NGG |
2 |
0.0103 |
Tier II |
| 14 |
NC_000001.11 |
1 |
232086208 |
- |
LOC105373171 |
NNNTGTATGCCTAATTCAAA |
NGG |
2 |
0.1657 |
Tier III |
| 15 |
NC_000009.12 |
9 |
82060298 |
- |
SPATA31B1P |
NNNTGTCTGCCCAATTCAAA |
NGG |
2 |
0.1346 |
Tier III |
| 16 |
NC_000009.12 |
9 |
82060298 |
- |
LOC105376107 |
NNNTGTCTGCCCAATTCAAA |
NGG |
2 |
0.1346 |
Tier III |
| 17 |
NC_000009.12 |
9 |
92885922 |
+ |
LOC642943 |
NNNTGTCTGCCCAATTCAAA |
NGG |
2 |
0.1346 |
Tier III |
| 18 |
NC_000008.11 |
8 |
129229618 |
- |
LINC00977 |
NNNTCTATGCCCAAGCCAAA |
NGG |
2 |
0.0393 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
161099589 |
- |
Klhl20 |
NNNTGTATGCCAAATCCAAA |
NGG |
1 |
0.7143 |
Tier II |
| 2 |
NC_000073.6 |
7 |
119851554 |
- |
Rexo5 |
NNNTGAATGCCCAATCTAAA |
NGG |
2 |
0.4044 |
Tier II |
| 3 |
NC_000086.7 |
X |
84788511 |
+ |
Dmd |
NNNTGTATCCCCGATCCAAA |
NGG |
2 |
0.3512 |
Tier II |
| 4 |
NC_000080.6 |
14 |
21271055 |
+ |
Adk |
NNNTGTATCCCTAATCCAAA |
NGG |
2 |
0.2899 |
Tier II |
| 5 |
NC_000071.6 |
5 |
134109224 |
- |
Castor2 |
NNNTGTATGCCCAATCCAGG |
NGG |
2 |
0.2868 |
Tier II |
| 6 |
NC_000078.6 |
12 |
111590243 |
+ |
Mark3 |
NNNTGTATTCCCAATCCTAA |
NGG |
2 |
0.2667 |
Tier II |
| 7 |
NC_000079.6 |
13 |
41818054 |
- |
Adtrp |
NNNTGGATGCCCAATCCAGA |
NGG |
2 |
0.2143 |
Tier II |
| 8 |
NC_000075.6 |
9 |
4743190 |
- |
Gria4 |
NNNTGTATGCTCAATCCAAT |
NGG |
2 |
0.1846 |
Tier II |
| 9 |
NC_000079.6 |
13 |
42777609 |
- |
Phactr1 |
NNNTGTATGCCCAACTCAAA |
NGG |
2 |
0.0839 |
Tier II |
| 10 |
NC_000071.6 |
5 |
103509223 |
- |
Ptpn13 |
NNNTGTGTGCCCAAGCCAAA |
NGG |
2 |
0.0353 |
Tier II |
| 11 |
NC_000086.7 |
X |
52564524 |
- |
Gpc3 |
NNNTGTATGCCCAATACAAA |
NGC |
2 |
0.0222 |
Tier II |
| 12 |
NC_000078.6 |
12 |
21162070 |
- |
Asap2 |
NNNTTTATGCCCAAGCCAAA |
NGG |
2 |
0.015 |
Tier II |
| 13 |
NC_000076.6 |
10 |
102779048 |
+ |
Gm5175 |
NNNTGTAGGTCCAATCCAAA |
NGG |
2 |
0.6902 |
Tier III |
| 14 |
NC_000067.6 |
1 |
184326995 |
+ |
Gm34177 |
NNNTGTATCCCCAAACCAAA |
NGG |
2 |
0.3117 |
Tier III |
Other clones with same target sequence:
(none)
