Construct: sgRNA BRDN0001145176

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCTTGGTAAATGATGAAGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAF1L (138474)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77121

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 32635289 - TAF1L NNNTGGTAAATGATGAAGGG NGG 0 1.0 Tier I
2 NC_000004.12 4 3235607 - HTT NNNTGGTAAATGATGGAGGG NGT 2 0.0031 Tier I
3 NC_000023.11 X 97420381 - DIAPH2 NNNTGATAAATGATGAAGGA NGG 2 0.9375 Tier II
4 NC_000019.10 19 13017560 + NFIX NNNTGGTAAAAAATGAAGGG NGG 2 0.7 Tier II
5 NC_000004.12 4 15729929 + BST1 NNNTGGAAAAAGATGAAGGG NGG 2 0.6563 Tier II
6 NC_000005.10 5 137258832 - SPOCK1 NNNTAGTGAATGATGAAGGG NGG 2 0.6356 Tier II
7 NC_000003.12 3 122799004 - SLC49A4 NNNTGGTAAATGAAAAAGGG NGG 2 0.5826 Tier II
8 NC_000017.11 17 9708195 + USP43 NNNGGGTAATTGATGAAGGG NGG 2 0.5515 Tier II
9 NC_000007.14 7 69754542 + AUTS2 NNNTGGTAGAAGATGAAGGG NGG 2 0.5 Tier II
10 NC_000006.12 6 15383415 - JARID2 NNNAGGTAAATGATGAAGGA NGG 2 0.4464 Tier II
11 NC_000004.12 4 46954674 - GABRA4 NNNTGTTAAATGATGAAGTG NGG 2 0.4444 Tier II
12 NC_000009.12 9 132203980 + NTNG2 NNNTGGGAGATGATGAAGGG NGG 2 0.3922 Tier II
13 NC_000023.11 X 85192130 - SATL1 NNNAGGTAAAAGATGAAGGG NGG 2 0.3571 Tier II
14 NC_000002.12 2 69211317 - ANTXR1 NNNTGGTAAGTGAAGAAGGG NGG 2 0.3439 Tier II
15 NC_000014.9 14 67360281 + GPHN NNNTGGTTAATGATGAAGAG NGG 2 0.3061 Tier II
16 NC_000006.12 6 31169113 - POU5F1 NNNTGCTAAATGATGAAGCG NGG 2 0.3056 Tier II
17 NC_000001.11 1 68184052 - WLS NNNTGGTAAATGATGAAGGG NAG 1 0.2593 Tier II
18 NC_000009.12 9 19890313 + SLC24A2 NNNTAGTAAATGATGAAGGG NAG 2 0.2247 Tier II
19 NC_000007.14 7 2132941 - MAD1L1 NNNTGGTAACTGATGAAGTG NGG 2 0.2222 Tier II
20 NC_000012.12 12 29619945 + TMTC1 NNNAGGTAAAGGATGAAGGG NGG 2 0.1905 Tier II
21 NC_000017.11 17 10116891 + GAS7 NNNTGATAAATGATGAGGGG NGG 2 0.1765 Tier II
22 NC_000023.11 X 17178488 + REPS2 NNNTGGTAAATGATGAATGG NAG 2 0.1728 Tier II
23 NC_000016.10 16 25918602 + HS3ST4 NNNTGGTAAAGGATGAAGGC NGG 2 0.1714 Tier II
24 NC_000003.12 3 112109677 - C3orf52 NNNAGGTAAATGACGAAGGG NGG 2 0.1361 Tier II
25 NC_000011.10 11 16179617 + SOX6 NNNTGTTAAATGATGAGGGG NGG 2 0.1176 Tier II
26 NC_000015.10 15 61019984 - RORA NNNTGGTTAATGCTGAAGGG NGG 2 0.0902 Tier II
27 NC_000004.12 4 98264066 + RAP1GDS1 NNNTGGTTAATGATGAGGGG NGG 2 0.0756 Tier II
28 NC_000020.11 20 46176536 + CDH22 NNNAGGTAAATGATGATGGG NGG 2 0.0635 Tier II
29 NC_000023.11 X 47578884 - SYN1 NNNTGGGAAATGAGGAAGGG NGG 2 0.0 Tier II
30 NC_000007.14 7 117092532 + ST7 NNNTGGTAAATGAGGAAGGG NAG 2 0.0 Tier II
31 NC_000014.9 14 40578684 - LOC105370466 NNNTGGAAAATGATGAAGGG NGG 1 0.875 Tier III
32 NC_000006.12 6 133680420 + TARID NNNTGGAAAAAGATGAAGGG NGG 2 0.6563 Tier III
33 NC_000008.11 8 85515033 + LOC105375937 NNNTAGTGAATGATGAAGGG NGG 2 0.6356 Tier III
34 NC_000004.12 4 188487037 - LINC01060 NNNTGGGAAATGATGAAAGG NGG 2 0.4072 Tier III
35 NC_000002.12 2 69211317 - LOC124906019 NNNTGGTAAGTGAAGAAGGG NGG 2 0.3439 Tier III
36 NC_000001.11 1 68184052 - GNG12-AS1 NNNTGGTAAATGATGAAGGG NAG 1 0.2593 Tier III
37 NC_000001.11 1 165133546 - LOC107985452 NNNTGGTAAAGGAAGAAGGG NGG 2 0.2476 Tier III
38 NC_000006.12 6 93504819 - LOC105377899 NNNTGGTAAATGATGAGAGG NGG 2 0.1222 Tier III
39 NC_000015.10 15 61019984 - LOC107984805 NNNTGGTTAATGCTGAAGGG NGG 2 0.0902 Tier III
40 NC_000002.12 2 48121486 - LOC105374593 NNNTGGTACATGATGAAGGG NGA 2 0.0397 Tier III
41 NC_000006.12 6 40378972 + TDRG1 NNNTGGTAACTGATGAAGGG NTG 2 0.013 Tier III
42 NC_000007.14 7 117092532 + ST7-AS2 NNNTGGTAAATGAGGAAGGG NAG 2 0.0 Tier III
43 NC_000007.14 7 117092532 + LOC124901732 NNNTGGTAAATGAGGAAGGG NAG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 10098551 + Itih2 NNNAGGTAAATGATGAAGTG NGG 2 0.3175 Tier I
2 NC_000071.6 5 34905573 - Htt NNNTGGTAAATGATGGAGGG NGT 2 0.0031 Tier I
3 NC_000086.7 X 140037218 + Nup62cl NNNTGGTAAATGATGAAGGA NGG 1 0.9375 Tier II
4 NC_000072.6 6 47007943 - Cntnap2 NNNTGGAAAATAATGAAGGG NGG 2 0.8167 Tier II
5 NC_000084.6 18 57909442 - Slc12a2 NNNTGATAAATGATGAAGGT NGG 2 0.7 Tier II
6 NC_000067.6 1 134657778 + Syt2 NNNTAGTAAAAGATGAAGGG NGG 2 0.65 Tier II
7 NC_000078.6 12 84214283 - Mideas NNNTGGTAAACGATGAATGG NGG 2 0.4444 Tier II
8 NC_000070.6 4 63059076 - Zfp618 NNNTGGGAAAAGATGAAGGG NGG 2 0.4412 Tier II
9 NC_000076.6 10 62622248 + Ddx50 NNNTGGTAAATGAAGAAGGT NGG 2 0.4333 Tier II
10 NC_000071.6 5 36497684 + Tbc1d14 NNNTGTTATATGATGAAGGG NGG 2 0.4 Tier II
11 NC_000080.6 14 122740208 + Pcca NNNTGGTAAAGGATGAAGGA NGG 2 0.375 Tier II
12 NC_000082.6 16 33872423 - Itgb5 NNNTGGTAAATATTGAAGGG NGG 2 0.28 Tier II
13 NC_000070.6 4 109112249 + Osbpl9 NNNTGGAAAATGTTGAAGGG NGG 2 0.2625 Tier II
14 NC_000078.6 12 38345233 - Dgkb NNNTTGTAAATGATGAAGAG NGG 2 0.2143 Tier II
15 NC_000084.6 18 77548476 - Rnf165 NNNTGGTGAATGCTGAAGGG NGG 2 0.1544 Tier II
16 NC_000073.6 7 57775048 + Gabrb3 NNNTGGTATATGCTGAAGGG NGG 2 0.1263 Tier II
17 NC_000069.6 3 84233918 + Trim2 NNNTGGTATATGATGAAGGG NGA 2 0.0417 Tier II
18 NC_000069.6 3 157274412 + Negr1 NNNTGGTAAAAGATGAAGGG NTG 2 0.0292 Tier II
19 NC_000067.6 1 65187515 + Pikfyve NNNTGTTAAATGATGAAGGG NTG 2 0.026 Tier II
20 NC_000086.7 X 84282279 + Dmd NNNTGGTAAATGATGAAGGC NGT 2 0.0069 Tier II
21 NC_000068.7 2 113595965 - Fmn1 NNNTGGTAAATGCTGAAGGG NGC 2 0.0047 Tier II
22 NC_000068.7 2 144481891 + Dzank1 NNNTGGAAAATGAGGAAGGG NGG 2 0.0 Tier II
23 NC_000079.6 13 8291521 + Adarb2 NNNTGGTAAATGAGGAAGGG NGA 2 0.0 Tier II
24 NC_000068.7 2 40778394 + Lrp1b NNNTGGTAAATGATGAAGGG NAT 2 0.0 Tier II
25 NC_000071.6 5 123493460 - Lrrc43 NNNTGGTAAGTGATGTAGGG NGG 2 0.0 Tier II
26 NC_000072.6 6 47007943 - Gm35875 NNNTGGAAAATAATGAAGGG NGG 2 0.8167 Tier III
27 NC_000078.6 12 84214283 - Gm51953 NNNTGGTAAACGATGAATGG NGG 2 0.4444 Tier III
28 NC_000080.6 14 74621202 + Gm41210 NNNAGGAAAATGATGAAGGG NGG 2 0.4167 Tier III
29 NC_000069.6 3 122300878 - Gm40190 NNNTGGTAAAGGATGAAGGG NGG 1 0.4 Tier III
30 NC_000076.6 10 43598874 - F930017D23Rik NNNTGGGAAATGGTGAAGGG NGG 2 0.3836 Tier III
31 NC_000076.6 10 61036271 - Gm20611 NNNTGGGAAATGATGAAGGC NGG 2 0.2521 Tier III
32 NC_000075.6 9 46498022 - Gm31432 NNNTGGTAAGTGATGGAGGG NGG 2 0.1068 Tier III
33 NC_000069.6 3 84233918 + Gm40078 NNNTGGTATATGATGAAGGG NGA 2 0.0417 Tier III
34 NC_000068.7 2 122583398 + Gm34325 NNNTTGTAAATGATGAAGGG NGT 2 0.0048 Tier III
35 NC_000081.6 15 62143062 - Pvt1 NNNTGTTAAATGAGGAAGGG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)