Construct: sgRNA BRDN0001145177
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACCGGATTATATATCACCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ROCK2 (9475)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76351
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
11227347 |
- |
ROCK2 |
NNNGGATTATATATCACCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
38852157 |
+ |
SCN11A |
NNNGAATTATATATCACTTG |
NGG |
2 |
0.5571 |
Tier II |
| 3 |
NC_000023.11 |
X |
70770306 |
- |
TEX11 |
NNNGGAATATATATCACCAG |
NGG |
2 |
0.25 |
Tier II |
| 4 |
NC_000016.10 |
16 |
73324056 |
+ |
ZFHX3 |
NNNGGATTATCTATCACATG |
NGG |
2 |
0.2154 |
Tier II |
| 5 |
NC_000001.11 |
1 |
71552181 |
- |
NEGR1 |
NNNTGATTACATATCACCTG |
NGG |
2 |
0.1939 |
Tier II |
| 6 |
NC_000007.14 |
7 |
31957438 |
+ |
PDE1C |
NNNGGATTATCTATCACCTG |
NAG |
2 |
0.1037 |
Tier II |
| 7 |
NC_000011.10 |
11 |
131693563 |
+ |
NTM |
NNNGGATTATCTATTACCTG |
NGG |
2 |
0.0267 |
Tier II |
| 8 |
NC_000023.11 |
X |
65040198 |
- |
ZC3H12B |
NNNGGCTTATATATGACCTG |
NGG |
2 |
0.0227 |
Tier II |
| 9 |
NC_000014.9 |
14 |
44929636 |
+ |
KLHL28 |
NNNGGAATATATATCTCCTG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000023.11 |
X |
48764887 |
- |
GLOD5 |
NNNGGATTATAAAGCACCTG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000016.10 |
16 |
80127795 |
- |
LOC105371357 |
NNNGCTTTATATATCACCTG |
NGG |
2 |
0.5612 |
Tier III |
| 12 |
NC_000021.9 |
21 |
23708909 |
- |
LOC105372749 |
NNNGGAATATATTTCACCTG |
NGG |
2 |
0.2625 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
107331988 |
- |
Rbm15 |
NNNGGATGAGATATCACCTG |
NGG |
2 |
0.3667 |
Tier I |
| 2 |
NC_000069.6 |
3 |
156596773 |
- |
Negr1 |
NNNGAATTATACATCACCTG |
NGG |
2 |
0.8211 |
Tier II |
| 3 |
NC_000069.6 |
3 |
156598303 |
+ |
Negr1 |
NNNGAATTATACATCACCTG |
NGG |
2 |
0.8211 |
Tier II |
| 4 |
NC_000067.6 |
1 |
151385849 |
- |
Swt1 |
NNNGGATAATATATCAACTG |
NGG |
2 |
0.3733 |
Tier II |
| 5 |
NC_000085.6 |
19 |
46460639 |
+ |
Sufu |
NNNGTATTATATATCACCTA |
NGG |
2 |
0.2813 |
Tier II |
| 6 |
NC_000077.6 |
11 |
49278257 |
- |
Olfr1393 |
NNNGAATTATTTATCACCTG |
NGG |
2 |
0.2667 |
Tier II |
| 7 |
NC_000068.7 |
2 |
142035093 |
+ |
Macrod2 |
NNNCGATTATAGATCACCTG |
NGG |
2 |
0.2647 |
Tier II |
| 8 |
NC_000079.6 |
13 |
99374766 |
- |
Mrps27 |
NNNGGATTATCTATCACCTC |
NGG |
2 |
0.1714 |
Tier II |
| 9 |
NC_000084.6 |
18 |
12470158 |
+ |
Lama3 |
NNNGGATTATATATCACATG |
NAG |
2 |
0.1396 |
Tier II |
| 10 |
NC_000070.6 |
4 |
85731004 |
- |
Adamtsl1 |
NNNGGTTTATATATCACCTG |
NGA |
2 |
0.0496 |
Tier II |
| 11 |
NC_000075.6 |
9 |
29645509 |
- |
Ntm |
NNNGGATTATCTATTACCTG |
NGG |
2 |
0.0267 |
Tier II |
| 12 |
NC_000076.6 |
10 |
61354395 |
- |
Pald1 |
NNNGGATTTTATATCACCTG |
NTG |
2 |
0.0234 |
Tier II |
| 13 |
NC_000078.6 |
12 |
110536735 |
- |
Ppp2r5c |
NNNGCATTATATAGCACCTG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
