Construct: sgRNA BRDN0001145178
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTATGGTTATGGAACCGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K20 (51776)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75596
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
173191062 |
- |
MAP3K20 |
NNNATGGTTATGGAACCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
70575053 |
- |
AUTS2 |
NNNCTGGTTATGCAACCGAG |
NGG |
2 |
0.1486 |
Tier II |
| 3 |
NC_000005.10 |
5 |
158748357 |
+ |
EBF1 |
NNNATGGTTATGGAATCCAG |
NGG |
2 |
0.1465 |
Tier II |
| 4 |
NC_000006.12 |
6 |
10881984 |
+ |
GCM2 |
NNNATGGTTATGGACCCGGG |
NGG |
2 |
0.1023 |
Tier II |
| 5 |
NC_000005.10 |
5 |
84174467 |
+ |
EDIL3 |
NNNATGGTTATGGAATGGAG |
NGG |
2 |
0.0181 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
72371893 |
- |
Map3k20 |
NNNGTGGTTATGGAACCGCG |
NGG |
2 |
0.1293 |
Tier I |
| 2 |
NC_000070.6 |
4 |
58900511 |
- |
Ecpas |
NNNATACTTATGGAACCGAG |
NGG |
2 |
0.6875 |
Tier II |
| 3 |
NC_000081.6 |
15 |
80329947 |
+ |
Cacna1i |
NNNATGGTTAAGGAACTGAG |
NGG |
2 |
0.35 |
Tier II |
| 4 |
NC_000070.6 |
4 |
98699976 |
- |
Patj |
NNNATGCTTATGGAACTGAG |
NGG |
2 |
0.3208 |
Tier II |
| 5 |
NC_000068.7 |
2 |
14626719 |
- |
Cacnb2 |
NNNATGGTTATGGAACCAGG |
NGG |
2 |
0.2596 |
Tier II |
| 6 |
NC_000079.6 |
13 |
45886440 |
- |
Atxn1 |
NNNATGGTTATGGAACACAG |
NGG |
2 |
0.2222 |
Tier II |
| 7 |
NC_000071.6 |
5 |
131643564 |
+ |
Auts2 |
NNNCTGGTTATGCAACCGAG |
NGG |
2 |
0.1486 |
Tier II |
| 8 |
NC_000082.6 |
16 |
44037737 |
- |
Gramd1c |
NNNATGGTTATGGTACCGAG |
NGT |
2 |
0.0086 |
Tier II |
| 9 |
NC_000071.6 |
5 |
105402661 |
- |
Gm26519 |
NNNATGGTTATGGAACCGTT |
NGG |
2 |
0.3769 |
Tier III |
| 10 |
NC_000070.6 |
4 |
98699976 |
- |
Gm35073 |
NNNATGCTTATGGAACTGAG |
NGG |
2 |
0.3208 |
Tier III |
| 11 |
NC_000084.6 |
18 |
26305412 |
+ |
Gm41679 |
NNNTTGGTTATGGAACAGAG |
NGG |
2 |
0.297 |
Tier III |
| 12 |
NC_000084.6 |
18 |
10898470 |
+ |
Gm35814 |
NNNCTGGTTCTGGAACCGAG |
NGG |
2 |
0.1176 |
Tier III |
| 13 |
NC_000067.6 |
1 |
91295002 |
- |
Gm7785 |
NNNATAGTTATGGAACCGAG |
NGC |
2 |
0.0222 |
Tier III |
Other clones with same target sequence:
(none)
