Construct: sgRNA BRDN0001145186

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAGAACGACCATATCCGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGF1R (3480)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76692

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 98913238 + IGF1R NNNGAACGACCATATCCGTG NGG 0 1.0 Tier I
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 68187184 + Igf1r NNNAAACGACCATATCCGTG NGG 1 0.9 Tier I
2 NC_000067.6 1 80723337 + Dock10 NNNGAATGACCAGATCCGTG NGG 2 0.212 Tier II
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Other clones with same target sequence:

(none)