Construct: sgRNA BRDN0001145187
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTACTCGCACCTGAACATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PFKL (5211)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77411
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
44313023 |
+ |
PFKL |
NNNCTCGCACCTGAACATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
27363924 |
+ |
MAP3K6 |
NNNCACGCACCTGCACATCG |
NGG |
2 |
0.1071 |
Tier I |
| 3 |
NC_000024.10 |
Y |
6416008 |
+ |
TTTY2B |
NNNCTCCCACCTGAACATTG |
NGG |
2 |
0.3173 |
Tier III |
| 4 |
NC_000024.10 |
Y |
9748712 |
- |
TTTY2 |
NNNCTCCCACCTGAACATTG |
NGG |
2 |
0.3173 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
4541540 |
+ |
Pcx |
NNNCTCACACCTGAACATCG |
NAG |
2 |
0.2593 |
Tier II |
| 2 |
NC_000074.6 |
8 |
110988725 |
- |
Ddx19a |
NNNCTCCCACCTGAACCTCG |
NGG |
2 |
0.1213 |
Tier II |
| 3 |
NC_000073.6 |
7 |
79247251 |
+ |
Gm39040 |
NNNCTCACACCTGAACATCC |
NGG |
2 |
0.4286 |
Tier III |
| 4 |
NC_000084.6 |
18 |
90017974 |
+ |
Gm6173 |
NNNCTTGCACCTGAACATGG |
NGG |
2 |
0.1161 |
Tier III |
Other clones with same target sequence:
(none)
