Construct: sgRNA BRDN0001145195
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCCAGGTCACCAATTGTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ATR (545)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77550
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
142519725 |
- |
ATR |
NNNCAGGTCACCAATTGTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
123716080 |
+ |
MYLK |
NNNCAGATCACCAATTGAGG |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000002.12 |
2 |
120089367 |
+ |
EPB41L5 |
NNNCTGGTCACCAATTTTGG |
NGG |
2 |
0.0909 |
Tier II |
| 4 |
NC_000009.12 |
9 |
92510649 |
- |
ECM2 |
NNNCAGGTCCCCACTTGTGG |
NGG |
2 |
0.0714 |
Tier II |
| 5 |
NC_000009.12 |
9 |
92510649 |
- |
CENPP |
NNNCAGGTCCCCACTTGTGG |
NGG |
2 |
0.0714 |
Tier II |
| 6 |
NC_000010.11 |
10 |
1301896 |
- |
ADARB2 |
NNNAAGGTCACCAATTGTGG |
NGA |
2 |
0.0585 |
Tier II |
| 7 |
NC_000012.12 |
12 |
132431895 |
+ |
LOC105370092 |
NNNCAGGTCACAAAGTGTGG |
NGG |
2 |
0.0357 |
Tier II |
| 8 |
NC_000013.11 |
13 |
36994928 |
- |
ALG5 |
NNNCAGGTCACCAATTGTGG |
NCC |
2 |
0.0 |
Tier II |
| 9 |
NC_000003.12 |
3 |
123716080 |
+ |
LOC105369194 |
NNNCAGATCACCAATTGAGG |
NGG |
2 |
0.6667 |
Tier III |
| 10 |
NC_000002.12 |
2 |
3009292 |
- |
LINC01250 |
NNNCAGGTCACCAATTGTCC |
NGG |
2 |
0.1921 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
62636285 |
- |
6720489N17Rik |
NNNTAGATCACCAATTGTGG |
NGG |
2 |
0.8 |
Tier II |
| 2 |
NC_000074.6 |
8 |
32880789 |
+ |
Nrg1 |
NNNGAAGTCACCAATTGTGG |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000079.6 |
13 |
62360508 |
- |
LOC115488203 |
NNNGAGATCACCAATTGTGG |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000079.6 |
13 |
62601357 |
- |
6720489N17Rik |
NNNGAGATCACCAATTGTGG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000078.6 |
12 |
116313088 |
+ |
Esyt2 |
NNNCAGGTCACCAAATGAGG |
NGG |
2 |
0.386 |
Tier II |
| 6 |
NC_000075.6 |
9 |
103537172 |
+ |
Tmem108 |
NNNCAGGTCACCAATCTTGG |
NGG |
2 |
0.1667 |
Tier II |
| 7 |
NC_000081.6 |
15 |
81891458 |
+ |
Aco2 |
NNNGAGGTCACCTATTGTGG |
NGG |
2 |
0.15 |
Tier II |
| 8 |
NC_000082.6 |
16 |
7133547 |
+ |
Rbfox1 |
NNNCAGGTCATCAACTGTGG |
NGG |
2 |
0.0839 |
Tier II |
| 9 |
NC_000070.6 |
4 |
121194316 |
- |
Rlf |
NNNCAGGTCCCCAATTTTGG |
NGG |
2 |
0.0833 |
Tier II |
| 10 |
NC_000082.6 |
16 |
4330088 |
- |
Adcy9 |
NNNCAGGTCCCCCATTGTGG |
NGG |
2 |
0.0702 |
Tier II |
| 11 |
NC_000077.6 |
11 |
5721918 |
- |
Urgcp |
NNNCAGGTCATCAAGTGTGG |
NGG |
2 |
0.0154 |
Tier II |
| 12 |
NC_000077.6 |
11 |
75233428 |
+ |
Rtn4rl1 |
NNNCAGGTCATCAAGTGTGG |
NGG |
2 |
0.0154 |
Tier II |
| 13 |
NC_000074.6 |
8 |
83656272 |
- |
Gipc1 |
NNNCAGGTCACCAAGTTTGG |
NGG |
2 |
0.0125 |
Tier II |
| 14 |
NC_000067.6 |
1 |
71376611 |
+ |
Abca12 |
NNNCAGGTCACCCAGTGTGG |
NGG |
2 |
0.0105 |
Tier II |
| 15 |
NC_000079.6 |
13 |
62348191 |
- |
Gm19792 |
NNNTAGATCACCAATTGTGG |
NGG |
2 |
0.8 |
Tier III |
| 16 |
NC_000079.6 |
13 |
61822332 |
+ |
Gm7240 |
NNNCAGATCACCAATTGTGC |
NGG |
2 |
0.4286 |
Tier III |
| 17 |
NC_000069.6 |
3 |
38205314 |
- |
5430434I15Rik |
NNNCAGGTCACCAATCTTGG |
NGG |
2 |
0.1667 |
Tier III |
| 18 |
NC_000075.6 |
9 |
103537172 |
+ |
Gm32743 |
NNNCAGGTCACCAATCTTGG |
NGG |
2 |
0.1667 |
Tier III |
| 19 |
NC_000076.6 |
10 |
91948534 |
- |
Gm31592 |
NNNCAGGACACCAAGTGTGG |
NGG |
2 |
0.04 |
Tier III |
| 20 |
NC_000067.6 |
1 |
105426526 |
- |
Gm28403 |
NNNCAGGTCACCTATTGTGG |
NTG |
2 |
0.0117 |
Tier III |
Other clones with same target sequence:
(none)
