Construct: sgRNA BRDN0001145196
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGCCAGGACGACCCCACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MPP3 (4356)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77811
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
43820939 |
- |
MPP3 |
NNNGCCAGGACGACCCCACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
19373685 |
+ |
MAP3K15 |
NNNGCCAGGACGACACCGCG |
NGG |
2 |
0.0889 |
Tier I |
| 3 |
NC_000001.11 |
1 |
24160130 |
- |
IFNLR1 |
NNNGCCAGGGCGACCCCAAG |
NGG |
2 |
0.2381 |
Tier II |
| 4 |
NC_000019.10 |
19 |
12786625 |
- |
HOOK2 |
NNNGCCAGGCCGGCCCCACG |
NGG |
2 |
0.2174 |
Tier II |
| 5 |
NC_000009.12 |
9 |
137253806 |
- |
CIMIP2A |
NNNGCCAGGCCGACCCCACC |
NGG |
2 |
0.1429 |
Tier II |
| 6 |
NC_000016.10 |
16 |
85566411 |
- |
GSE1 |
NNNGCCAGGACGACCTCACC |
NGG |
2 |
0.1319 |
Tier II |
| 7 |
NC_000004.12 |
4 |
3387442 |
+ |
RGS12 |
NNNGCCAGGAGGACCCCAGG |
NGG |
2 |
0.0313 |
Tier II |
| 8 |
NC_000019.10 |
19 |
35995949 |
+ |
SDHAF1 |
NNNGCCAGGACGACCCCCGG |
NGG |
2 |
0.0238 |
Tier II |
| 9 |
NC_000019.10 |
19 |
33797184 |
- |
KCTD15 |
NNNGCCAGGACCAGCCCACG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000001.11 |
1 |
3747045 |
+ |
GFOD3P |
NNNGCCAGGACGATCCCACC |
NGG |
2 |
0.3143 |
Tier III |
| 11 |
NC_000001.11 |
1 |
24160130 |
- |
LOC124903879 |
NNNGCCAGGGCGACCCCAAG |
NGG |
2 |
0.2381 |
Tier III |
| 12 |
NC_000012.12 |
12 |
63288164 |
- |
LINC03056 |
NNNGCCAAGACGACACCACG |
NGG |
2 |
0.2222 |
Tier III |
| 13 |
NC_000012.12 |
12 |
54391423 |
- |
GPR84-AS1 |
NNNGCCAGGACGCCCCCTCG |
NGG |
2 |
0.1053 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
123958424 |
- |
Abcb10 |
NNNCCCAGGACGTCCCCACG |
NGG |
2 |
0.1588 |
Tier II |
| 2 |
NC_000082.6 |
16 |
55858635 |
- |
Nxpe3 |
NNNGCCAGGACGACCTCAAG |
NGG |
2 |
0.1319 |
Tier II |
| 3 |
NC_000077.6 |
11 |
100870734 |
- |
Stat5a |
NNNGCCAGGACGAGCCCACG |
NGA |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
