Construct: sgRNA BRDN0001145198
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAATAACATCTAAACACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LMTK2 (22853)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76173
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
98192995 |
- |
LMTK2 |
NNNATAACATCTAAACACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
174643505 |
+ |
GLRA3 |
NNNATAACATATAAACACAT |
NGG |
2 |
0.5357 |
Tier II |
| 3 |
NC_000003.12 |
3 |
77468985 |
- |
ROBO2 |
NNNATAACATCTGAACATGT |
NGG |
2 |
0.4193 |
Tier II |
| 4 |
NC_000001.11 |
1 |
210896056 |
+ |
KCNH1 |
NNNATAACAGCTAAACATGT |
NGG |
2 |
0.3214 |
Tier II |
| 5 |
NC_000011.10 |
11 |
117046940 |
+ |
SIK3 |
NNNATAAAATCTAAACACCT |
NGG |
2 |
0.2914 |
Tier II |
| 6 |
NC_000003.12 |
3 |
31759125 |
+ |
OSBPL10 |
NNNATAACATGTAAACATGT |
NGG |
2 |
0.1607 |
Tier II |
| 7 |
NC_000016.10 |
16 |
70895112 |
+ |
HYDIN |
NNNATGACATCTAAACACGT |
NGC |
2 |
0.0159 |
Tier II |
| 8 |
NC_000001.11 |
1 |
24837971 |
+ |
CLIC4 |
NNNATAACTTCTAAACACGT |
NGC |
2 |
0.0133 |
Tier II |
| 9 |
NC_000001.11 |
1 |
70825080 |
+ |
LOC102724572 |
NNNATAACTTCTAAGCACGT |
NGG |
2 |
0.39 |
Tier III |
| 10 |
NC_000001.11 |
1 |
96304863 |
+ |
LINC01787 |
NNNATAACATCTAAAAACGT |
NTG |
2 |
0.039 |
Tier III |
| 11 |
NC_000001.11 |
1 |
146820459 |
- |
HYDIN2 |
NNNATGACATCTAAACACGT |
NGC |
2 |
0.0159 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
86066860 |
+ |
Sh3d19 |
NNNATAACATCTAAACAAGA |
NGG |
2 |
0.3029 |
Tier II |
| 2 |
NC_000076.6 |
10 |
70954734 |
+ |
Bicc1 |
NNNATAACACCTAAACACCT |
NGG |
2 |
0.2391 |
Tier II |
| 3 |
NC_000082.6 |
16 |
55823043 |
+ |
Nfkbiz |
NNNGTAACATCTTAACACGT |
NGG |
2 |
0.1875 |
Tier II |
| 4 |
NC_000068.7 |
2 |
61128437 |
- |
Gm39833 |
NNNATAAAATCTAAACATGT |
NGG |
2 |
0.4179 |
Tier III |
| 5 |
NC_000072.6 |
6 |
124103979 |
+ |
Vmn2r-ps33 |
NNNATAAGATCTAAACACGT |
NGT |
2 |
0.0104 |
Tier III |
Other clones with same target sequence:
(none)
