Construct: sgRNA BRDN0001145205
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTGGCAAATTCGTAAACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3R4 (30849)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75592
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
130723438 |
+ |
PIK3R4 |
NNNGGCAAATTCGTAAACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
55384008 |
- |
GFRAL |
NNNAGCAAATTCGAAAACAT |
NGG |
2 |
0.5571 |
Tier II |
| 3 |
NC_000021.9 |
21 |
21071333 |
+ |
NCAM2 |
NNNAGCAAATTGGTAAACAT |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000015.10 |
15 |
92423107 |
+ |
ST8SIA2 |
NNNGGCAAATTCATAAAGAT |
NGG |
2 |
0.1231 |
Tier II |
| 5 |
NC_000015.10 |
15 |
40846474 |
- |
SPINT1 |
NNNGGCAAATTCTTTAACAT |
NGG |
2 |
0.06 |
Tier II |
| 6 |
NC_000003.12 |
3 |
144470183 |
+ |
LOC105374140 |
NNNGGCTATTTCGTAAACAT |
NGG |
2 |
0.2625 |
Tier III |
| 7 |
NC_000003.12 |
3 |
80824587 |
+ |
LOC105377174 |
NNNGGCAAATTCTTAAAGAT |
NGG |
2 |
0.04 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
111956073 |
- |
Tex16 |
NNNGGCAAATGGGTAAACAT |
NGG |
2 |
0.1778 |
Tier II |
| 2 |
NC_000071.6 |
5 |
27334087 |
- |
Dpp6 |
NNNGGCAAATTCTTAGACAT |
NGG |
2 |
0.0577 |
Tier II |
Other clones with same target sequence:
(none)
