Construct: sgRNA BRDN0001145207
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGTCTGACGAGATGTTGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ULK2 (9706)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77260
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
19816771 |
+ |
ULK2 |
NNNTCTGACGAGATGTTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
4556978 |
- |
ITPR1 |
NNNTCTGACTAGGTGTTGTG |
NGG |
2 |
0.5299 |
Tier II |
| 3 |
NC_000007.14 |
7 |
126463024 |
+ |
GRM8 |
NNNTCTGACTAGATGTAGTG |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000017.11 |
17 |
16046609 |
+ |
NCOR1 |
NNNTCTGAGGAGATGTTATG |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000001.11 |
1 |
102966339 |
- |
COL11A1 |
NNNTCTGACCAGATGTTGTA |
NGG |
2 |
0.375 |
Tier II |
| 6 |
NC_000004.12 |
4 |
7469008 |
+ |
SORCS2 |
NNNTCTGAAGAGTTGTTGTG |
NGG |
2 |
0.2571 |
Tier II |
| 7 |
NC_000006.12 |
6 |
101182605 |
+ |
LOC107984041 |
NNNTCTGAAGAGATGTTGTG |
NGG |
1 |
0.8571 |
Tier III |
| 8 |
NC_000008.11 |
8 |
136632061 |
+ |
LINC02055 |
NNNTCTGAAGAGATGTTGTG |
NGG |
1 |
0.8571 |
Tier III |
| 9 |
NC_000010.11 |
10 |
91049557 |
- |
LINC00502 |
NNNTCTGCCTAGATGTTGTG |
NGG |
2 |
0.3482 |
Tier III |
| 10 |
NC_000024.10 |
Y |
6471305 |
- |
TTTY8B |
NNNTCTGCCTAGATGTTGTG |
NGG |
2 |
0.3482 |
Tier III |
| 11 |
NC_000024.10 |
Y |
9693426 |
+ |
TTTY8 |
NNNTCTGCCTAGATGTTGTG |
NGG |
2 |
0.3482 |
Tier III |
| 12 |
NC_000009.12 |
9 |
84549680 |
+ |
LOC102724036 |
NNNTCTGACTTGATGTTGTG |
NGG |
2 |
0.25 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
61819307 |
+ |
Ulk2 |
NNNTCTGACGAGATGTTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
90838977 |
+ |
Dlg2 |
NNNTCTGACTACATGTTGTG |
NGG |
2 |
0.4301 |
Tier II |
| 3 |
NC_000069.6 |
3 |
132908308 |
- |
Npnt |
NNNTCTGAGGAGATGCTGTG |
NGG |
2 |
0.4127 |
Tier II |
| 4 |
NC_000069.6 |
3 |
136805856 |
- |
Ppp3ca |
NNNTCTGAGGAGATGCTGTG |
NGG |
2 |
0.4127 |
Tier II |
| 5 |
NC_000071.6 |
5 |
65075077 |
- |
Tmem156 |
NNNTCTGATGTGATGTTGTG |
NGG |
2 |
0.2692 |
Tier II |
| 6 |
NC_000071.6 |
5 |
122297043 |
+ |
Pptc7 |
NNNTCTGACTAGATCTTGTG |
NGG |
2 |
0.2216 |
Tier II |
| 7 |
NC_000076.6 |
10 |
25192561 |
- |
Akap7 |
NNNTCTGACGAGGTGTTGAG |
NGG |
2 |
0.1863 |
Tier II |
| 8 |
NC_000070.6 |
4 |
151124441 |
- |
Camta1 |
NNNTCTGACCAGATGTGGTG |
NGG |
2 |
0.0471 |
Tier II |
| 9 |
NC_000072.6 |
6 |
125763831 |
+ |
Ano2 |
NNNACTGACGAGATGTTGTG |
NGC |
2 |
0.0106 |
Tier II |
| 10 |
NC_000068.7 |
2 |
131452054 |
- |
Gm14304 |
NNNTCTGACTAGATGTTGGG |
NGG |
2 |
0.2031 |
Tier III |
Other clones with same target sequence:
(none)
