Construct: sgRNA BRDN0001145208
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGTGTGTCAAACTCTCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PFKP (5214)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77627
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
3105497 |
+ |
PFKP |
NNNGTGTGTCAAACTCTCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
33879229 |
- |
ADAMTS12 |
NNNGTGTGTCAGAATCTCGG |
NGG |
2 |
0.2528 |
Tier II |
| 3 |
NC_000007.14 |
7 |
149007649 |
+ |
PDIA4 |
NNNGTGTGTCACACTCTAGG |
NGG |
2 |
0.1417 |
Tier II |
| 4 |
NC_000010.11 |
10 |
122010538 |
- |
TACC2 |
NNNGTGTGTCAACCTCTCTG |
NGG |
2 |
0.1404 |
Tier II |
| 5 |
NC_000003.12 |
3 |
58242137 |
+ |
ABHD6 |
NNNGTGTGTCAAAGTCCCGG |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
88453086 |
+ |
Sema4a |
NNNGCGTGTCAAAGTCTCGG |
NGG |
2 |
0.0 |
Tier I |
| 2 |
NC_000082.6 |
16 |
4629773 |
- |
Coro7 |
NNNGTGTGTTAAACTCTCAG |
NGG |
2 |
0.6723 |
Tier II |
| 3 |
NC_000077.6 |
11 |
93466110 |
+ |
Car10 |
NNNGTGTGTCACACTCTCTG |
NGG |
2 |
0.1754 |
Tier II |
| 4 |
NC_000074.6 |
8 |
48524684 |
- |
Tenm3 |
NNNGTGTGTCAAACTGTCCG |
NGG |
2 |
0.069 |
Tier II |
| 5 |
NC_000076.6 |
10 |
12061982 |
+ |
Gm40601 |
NNNATGGGTCAAACTCTCGG |
NGG |
2 |
0.5294 |
Tier III |
| 6 |
NC_000081.6 |
15 |
62259349 |
+ |
Pvt1 |
NNNGTGTGTCAAACTCCTGG |
NGG |
2 |
0.4538 |
Tier III |
Other clones with same target sequence:
(none)
