Construct: sgRNA BRDN0001145210
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGGCTACGGCAAGTTCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHB1 (2047)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77532
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
135162146 |
+ |
EPHB1 |
NNNGGCTACGGCAAGTTCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
5441959 |
- |
OR51B5 |
NNNGGCTATGACAAGTTCAG |
NGG |
2 |
0.875 |
Tier II |
| 3 |
NC_000010.11 |
10 |
114870769 |
+ |
FHIP2A |
NNNGGATAAGGCAAGTTCAG |
NGG |
2 |
0.7959 |
Tier II |
| 4 |
NC_000008.11 |
8 |
99639392 |
+ |
VPS13B |
NNNGGCTACAGCAAGCTCAG |
NGG |
2 |
0.6222 |
Tier II |
| 5 |
NC_000011.10 |
11 |
100232462 |
+ |
CNTN5 |
NNNGGCAAGGGCAAGTTCAG |
NGG |
2 |
0.5417 |
Tier II |
| 6 |
NC_000001.11 |
1 |
16966199 |
- |
CROCC |
NNNGGCCACGGCAAGGTCAG |
NGG |
2 |
0.238 |
Tier II |
| 7 |
NC_000004.12 |
4 |
176417887 |
- |
LOC124900817 |
NNNGGCTACAGCAAGATCAG |
NGG |
2 |
0.8485 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
102001740 |
- |
Ephb1 |
NNNGGCTATGGCAAGTTCAG |
NGG |
1 |
0.875 |
Tier I |
| 2 |
NC_000073.6 |
7 |
66794548 |
- |
Cers3 |
NNNGCCTACGGCAAGTTCAG |
NGG |
1 |
0.7857 |
Tier II |
| 3 |
NC_000073.6 |
7 |
80892826 |
+ |
Wdr73 |
NNNGGTTACTGCAAGTTCAG |
NGG |
2 |
0.7545 |
Tier II |
| 4 |
NC_000079.6 |
13 |
65181036 |
- |
Nlrp4f |
NNNGACTACGGCAAGTTCAT |
NGG |
2 |
0.6067 |
Tier II |
| 5 |
NC_000077.6 |
11 |
51352778 |
- |
Col23a1 |
NNNGGCTGCTGCAAGTTCAG |
NGG |
2 |
0.5958 |
Tier II |
| 6 |
NC_000076.6 |
10 |
31573012 |
+ |
Rnf217 |
NNNGGCTAGGGCAAGTTCAT |
NGG |
2 |
0.4333 |
Tier II |
| 7 |
NC_000071.6 |
5 |
53876051 |
- |
Tbc1d19 |
NNNGGCTACGGCAAGATCAG |
NTG |
2 |
0.0354 |
Tier II |
| 8 |
NC_000082.6 |
16 |
95240844 |
- |
Gm31641 |
NNNGACTTCGGCAAGTTCAG |
NGG |
2 |
0.3714 |
Tier III |
Other clones with same target sequence:
(none)
