Construct: sgRNA BRDN0001145211

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGAATTTCAGACCAACAAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NME1-NME2 (654364)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75885

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 51155759 - NME1 NNNATTTCAGACCAACAAGG NGG 0 1.0 Tier I
2 NC_000017.11 17 51155759 - NME1-NME2 NNNATTTCAGACCAACAAGG NGG 0 1.0 Tier I
3 NC_000006.12 6 80040661 - TTK NNNAATTCAGACCAACAAGT NGG 2 0.35 Tier I
4 NC_000019.10 19 17636151 - UNC13A NNNATTTCAGGCCAAGAAGG NGG 2 0.1 Tier I
5 NC_000002.12 2 162565084 + KCNH7 NNNATTTCATACCAACAAGT NGG 2 0.5688 Tier II
6 NC_000015.10 15 40779742 + DNAJC17 NNNATGACAGACCAACAAGG NGG 2 0.5 Tier II
7 NC_000012.12 12 113248636 + TPCN1 NNNGTTTCAGAACAACAAGG NGG 2 0.4464 Tier II
8 NC_000009.12 9 100252040 + INVS NNNATTTCAGACCAACAGGA NGG 2 0.375 Tier II
9 NC_000005.10 5 80060343 + THBS4 NNNATCTCACACCAACAAGG NGG 2 0.3636 Tier II
10 NC_000010.11 10 14297285 + FRMD4A NNNATTACAGACAAACAAGG NGG 2 0.3365 Tier II
11 NC_000023.11 X 74835533 - NEXMIF NNNATTTAAGACCACCAAGG NGG 2 0.1773 Tier II
12 NC_000008.11 8 28383173 + ZNF395 NNNATTTCAGAGAAACAAGG NGG 2 0.1709 Tier II
13 NC_000008.11 8 3016004 - CSMD1 NNNAATTCAGACCAATAAGG NGG 2 0.1538 Tier II
14 NC_000001.11 1 121462222 + LINC02798 NNNATTTCAGACCAAGAAGT NGG 2 0.1077 Tier II
15 NC_000002.12 2 100072631 + AFF3 NNNATTTCAGACCAAGAAGG NAG 2 0.0399 Tier II
16 NC_000006.12 6 157171770 + ARID1B NNNATTTCAGACCAACAATG NGT 2 0.0108 Tier II
17 NC_000006.12 6 129907539 + LOC105377999 NNNATCTCATACCAACAAGG NGG 2 0.7386 Tier III
18 NC_000017.11 17 5788078 - LOC339166 NNNATTTCAAACCAACAAGT NGG 2 0.6533 Tier III
19 NC_000008.11 8 122519909 + SMILR NNNATTTCAGACAAACAAAG NGG 2 0.2747 Tier III
20 NC_000010.11 10 90525505 - LINC02653 NNNATTTAAGACCACCAAGG NGG 2 0.1773 Tier III
21 NC_000008.11 8 3016004 - LOC105377785 NNNAATTCAGACCAATAAGG NGG 2 0.1538 Tier III
22 NC_000019.10 19 32029594 - LINC01533 NNNATTTCAGCCAAACAAGG NGG 2 0.1538 Tier III
23 NC_000019.10 19 32029594 - LINC01837 NNNATTTCAGCCAAACAAGG NGG 2 0.1538 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 93965830 + Nme1 NNNACTTCAGACCAACAAGG NGG 1 1.0 Tier I
2 NC_000071.6 5 99964090 + Hnrnpd NNNATTTCACACTAACAAGG NGG 2 0.28 Tier I
3 NC_000067.6 1 175438555 + Rgs7 NNNATTTCAGACCAGCAAGA NGG 2 0.6094 Tier II
4 NC_000067.6 1 152051650 + 1700025G04Rik NNNATACCAGACCAACAAGG NGG 2 0.5958 Tier II
5 NC_000080.6 14 21844613 - Vdac2 NNNATCTCAGACCTACAAGG NGG 2 0.4848 Tier II
6 NC_000075.6 9 8559051 + Trpc6 NNNATTTCACACCAAAAAGG NGG 2 0.4 Tier II
7 NC_000076.6 10 64585225 - Ctnna3 NNNATTTCAAACCACCAAGG NGG 2 0.2545 Tier II
8 NC_000085.6 19 22305850 + Trpm3 NNNTTTTCAGACCAACAAGG NAG 2 0.165 Tier II
9 NC_000078.6 12 88850645 + Nrxn3 NNNATTTCAGAGCAACCAGG NGG 2 0.0784 Tier II
10 NC_000074.6 8 29143706 + Unc5d NNNATTTTAGACCAACAAGG NGA 2 0.0608 Tier II
11 NC_000071.6 5 44689095 - Ldb2 NNNATTTCAGAGGAACAAGG NGG 2 0.0606 Tier II
12 NC_000081.6 15 11291161 + Adamts12 NNNATTTCAGACCAACAAGG NTG 1 0.039 Tier II
13 NC_000070.6 4 115578464 - Cyp4a31 NNNATTTCAGACCACCAAGG NTG 2 0.0106 Tier II
14 NC_000084.6 18 26052762 + Gm33228 NNNATTTTAGACCAAAAAGG NGG 2 0.875 Tier III
15 NC_000079.6 13 78275532 + Gm46387 NNNATTTCAAACCAGCAAGG NGG 2 0.6067 Tier III
16 NC_000068.7 2 6265885 - A230108P19Rik NNNATTTCAGTCCAACACGG NGG 2 0.0586 Tier III
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Other clones with same target sequence:

(none)