Construct: sgRNA BRDN0001145213
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTCCAGTCCAGGATAACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA3 (2042)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76742
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
89341806 |
- |
EPHA3 |
NNNCCAGTCCAGGATAACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
14378467 |
+ |
MACROD2 |
NNNTCAGTTCAGGATAACTG |
NGG |
2 |
0.7 |
Tier II |
| 3 |
NC_000001.11 |
1 |
20984005 |
+ |
EIF4G3 |
NNNCCTGTCCAGAATAACTG |
NGG |
2 |
0.6593 |
Tier II |
| 4 |
NC_000003.12 |
3 |
69419509 |
- |
FRMD4B |
NNNCCAGTGAAGGATAACTG |
NGG |
2 |
0.5365 |
Tier II |
| 5 |
NC_000019.10 |
19 |
5040919 |
+ |
KDM4B |
NNNCCGGGCCAGGATAACTG |
NGG |
2 |
0.5238 |
Tier II |
| 6 |
NC_000011.10 |
11 |
66436384 |
+ |
MRPL11 |
NNNCCAGTCCAGATTAACTG |
NGG |
2 |
0.4923 |
Tier II |
| 7 |
NC_000013.11 |
13 |
93532201 |
- |
GPC6 |
NNNACAGTCCAGGAAAACTG |
NGG |
2 |
0.4875 |
Tier II |
| 8 |
NC_000020.11 |
20 |
32522514 |
+ |
NOL4L |
NNNCCAGGCCAGGATAAATG |
NGG |
2 |
0.3949 |
Tier II |
| 9 |
NC_000014.9 |
14 |
92068486 |
+ |
ATXN3 |
NNNCAAGTCCAGGATAAATG |
NGG |
2 |
0.3077 |
Tier II |
| 10 |
NC_000011.10 |
11 |
10100424 |
- |
SBF2 |
NNNCCAGTCCAAGACAACTG |
NGG |
2 |
0.2545 |
Tier II |
| 11 |
NC_000007.14 |
7 |
117210342 |
- |
ST7 |
NNNCCAGTGGAGGATAACTG |
NGG |
2 |
0.2407 |
Tier II |
| 12 |
NC_000006.12 |
6 |
159912009 |
+ |
MAS1 |
NNNTCAGTCCAGGATAACGG |
NGG |
2 |
0.2 |
Tier II |
| 13 |
NC_000012.12 |
12 |
15367299 |
+ |
PTPRO |
NNNCCAGTCTAGGATGACTG |
NGG |
2 |
0.181 |
Tier II |
| 14 |
NC_000004.12 |
4 |
36164263 |
+ |
ARAP2 |
NNNGCAGTCCAGGATAACTG |
NAG |
2 |
0.1296 |
Tier II |
| 15 |
NC_000006.12 |
6 |
111453483 |
- |
REV3L |
NNNCAAGTCCAGGATGACTG |
NGG |
2 |
0.1099 |
Tier II |
| 16 |
NC_000014.9 |
14 |
72766777 |
- |
DPF3 |
NNNCCAGTCCAGGATATATG |
NGG |
2 |
0.0718 |
Tier II |
| 17 |
NC_000018.10 |
18 |
6101232 |
+ |
L3MBTL4 |
NNNCCAGTCCAGGATATATG |
NGG |
2 |
0.0718 |
Tier II |
| 18 |
NC_000012.12 |
12 |
57193446 |
- |
LRP1 |
NNNCCAGTCCAGGCTAACAG |
NGG |
2 |
0.0612 |
Tier II |
| 19 |
NC_000010.11 |
10 |
104217103 |
+ |
CFAP43 |
NNNCCAGTCCTGGATATCTG |
NGG |
2 |
0.041 |
Tier II |
| 20 |
NC_000022.11 |
22 |
37858970 |
- |
EIF3L |
NNNCCAGTCCAGGAGAATTG |
NGG |
2 |
0.0321 |
Tier II |
| 21 |
NC_000002.12 |
2 |
159114787 |
- |
TANC1 |
NNNCAAGTCCAGGAGAACTG |
NGG |
2 |
0.0286 |
Tier II |
| 22 |
NC_000002.12 |
2 |
30910805 |
+ |
GALNT14 |
NNNCCAGTCCAGGATGTCTG |
NGG |
2 |
0.0256 |
Tier II |
| 23 |
NC_000006.12 |
6 |
69969889 |
- |
COL19A1 |
NNNCAAGTCCAGGATAACTG |
NTG |
2 |
0.0223 |
Tier II |
| 24 |
NC_000004.12 |
4 |
173506828 |
+ |
SCRG1 |
NNNCCAGTCCAGGGTAACTG |
NGC |
2 |
0.0104 |
Tier II |
| 25 |
NC_000007.14 |
7 |
67205864 |
+ |
TYW1 |
NNNCCAGTCCAGGATATCTG |
NGC |
2 |
0.003 |
Tier II |
| 26 |
NC_000012.12 |
12 |
27447266 |
+ |
SMCO2 |
NNNACAGTCCAGGATTACTG |
NGG |
2 |
0.0 |
Tier II |
| 27 |
NC_000011.10 |
11 |
92446346 |
- |
FAT3 |
NNNCCAGTCCAGGATCACTG |
NGT |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
63652500 |
+ |
Epha3 |
NNNCCAGTCCAGGATAACCG |
NGG |
1 |
0.2759 |
Tier I |
| 2 |
NC_000077.6 |
11 |
21589907 |
+ |
Wdpcp |
NNNCTAGTCTAGGATAACTG |
NGG |
2 |
0.5989 |
Tier II |
| 3 |
NC_000083.6 |
17 |
57363660 |
- |
Adgre1 |
NNNCCAGTGTAGGATAACTG |
NGG |
2 |
0.5826 |
Tier II |
| 4 |
NC_000085.6 |
19 |
53319107 |
- |
Mxi1 |
NNNCCAGCCCAGGATAACAG |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000083.6 |
17 |
8632421 |
- |
Pde10a |
NNNCCAGTCCATGGTAACTG |
NGG |
2 |
0.1795 |
Tier II |
| 6 |
NC_000075.6 |
9 |
32343847 |
- |
Kcnj5 |
NNNCCAGACCAGGATACCTG |
NGG |
2 |
0.1412 |
Tier II |
| 7 |
NC_000071.6 |
5 |
121447120 |
- |
Erp29 |
NNNCAAGTCCAGGATGACTG |
NGG |
2 |
0.1099 |
Tier II |
| 8 |
NC_000082.6 |
16 |
28388973 |
- |
Fgf12 |
NNNCAAGTCCAGGATAGCTG |
NGG |
2 |
0.1008 |
Tier II |
| 9 |
NC_000079.6 |
13 |
114047740 |
+ |
Arl15 |
NNNCCAGTCCAGGTTAACTG |
NGC |
2 |
0.0119 |
Tier II |
| 10 |
NC_000077.6 |
11 |
86347132 |
- |
Med13 |
NNNCCAGTCCAGGACAACTG |
NGT |
2 |
0.0044 |
Tier II |
| 11 |
NC_000081.6 |
15 |
83176422 |
- |
Cyb5r3 |
NNNCCAGGCCAGGATCACTG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000083.6 |
17 |
66411276 |
+ |
Mtcl1 |
NNNCCAGTCCAGGATCTCTG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000080.6 |
14 |
109422851 |
+ |
Gm30216 |
NNNCCAGTCAAGGATAACTG |
NGG |
1 |
0.8667 |
Tier III |
| 14 |
NC_000085.6 |
19 |
53319107 |
- |
Gm30541 |
NNNCCAGCCCAGGATAACAG |
NGG |
2 |
0.2857 |
Tier III |
| 15 |
NC_000077.6 |
11 |
95313521 |
- |
Gm11520 |
NNNCCAGTCCTGGAAAACTG |
NGG |
2 |
0.1781 |
Tier III |
| 16 |
NC_000071.6 |
5 |
31597306 |
- |
Gm38424 |
NNNCCAGTCCAGGATAATGG |
NGG |
2 |
0.1607 |
Tier III |
Other clones with same target sequence:
(none)
