Construct: sgRNA BRDN0001145215
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCGGCTTGGAAGATTTACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRK4 (2868)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77723
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
3019809 |
+ |
GRK4 |
NNNGCTTGGAAGATTTACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
124540135 |
+ |
NDUFB9 |
NNNGCCTGGAAGAATTACAG |
NGG |
2 |
0.5628 |
Tier II |
| 3 |
NC_000004.12 |
4 |
146382121 |
- |
SLC10A7 |
NNNACTTGGAATATTTACAG |
NGG |
2 |
0.3462 |
Tier II |
| 4 |
NC_000012.12 |
12 |
106634332 |
- |
RFX4 |
NNNCTTTGGAAGATTTACAG |
NGG |
2 |
0.3369 |
Tier II |
| 5 |
NC_000009.12 |
9 |
114924921 |
+ |
TNFSF8 |
NNNCCTGGGAAGATTTACAG |
NGG |
2 |
0.3114 |
Tier II |
| 6 |
NC_000004.12 |
4 |
139540276 |
- |
SETD7 |
NNNGCTTGGAAGATTGACAG |
NAG |
2 |
0.0897 |
Tier II |
| 7 |
NC_000011.10 |
11 |
19536146 |
- |
NAV2 |
NNNGCTTGGAAGATTAACAG |
NGA |
2 |
0.0631 |
Tier II |
| 8 |
NC_000006.12 |
6 |
42212974 |
+ |
MRPS10 |
NNNGCTTGGAAGATTTAGAC |
NGG |
2 |
0.0571 |
Tier II |
| 9 |
NC_000005.10 |
5 |
803315 |
+ |
ZDHHC11 |
NNNCCTTGGAAGATTTACAG |
NGA |
2 |
0.0368 |
Tier II |
| 10 |
NC_000007.14 |
7 |
31596345 |
- |
ITPRID1 |
NNNGCTTGGAAGATTTACAA |
NTG |
2 |
0.0365 |
Tier II |
| 11 |
NC_000017.11 |
17 |
30500830 |
- |
GOSR1 |
NNNGATTGGAAGATTTACAG |
NTG |
2 |
0.0223 |
Tier II |
| 12 |
NC_000004.12 |
4 |
109272745 |
+ |
COL25A1 |
NNNGCTTGGAAGATTTGCAG |
NGA |
2 |
0.0123 |
Tier II |
| 13 |
NC_000010.11 |
10 |
117114844 |
- |
SHTN1 |
NNNGCTTGGAAGAATTACAG |
NGT |
2 |
0.01 |
Tier II |
| 14 |
NC_000015.10 |
15 |
92155464 |
+ |
SLCO3A1 |
NNNGGTTGGAAGAGTTACAG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000017.11 |
17 |
68835862 |
- |
LOC105371874 |
NNNGCTTGGAAGATTAAAAG |
NGG |
2 |
0.4895 |
Tier III |
| 16 |
NC_000012.12 |
12 |
106634332 |
- |
LOC100287944 |
NNNCTTTGGAAGATTTACAG |
NGG |
2 |
0.3369 |
Tier III |
| 17 |
NC_000007.14 |
7 |
142567194 |
- |
TRB |
NNNTCTTGGAAGATTTAAAG |
NGG |
2 |
0.1958 |
Tier III |
| 18 |
NC_000010.11 |
10 |
64103090 |
- |
LOC124902439 |
NNNGCTTGGAGGATTTACAG |
NAG |
2 |
0.1685 |
Tier III |
| 19 |
NC_000001.11 |
1 |
103519619 |
+ |
RNPC3-DT |
NNNGCTTGGAAGATATACAG |
NCG |
2 |
0.062 |
Tier III |
| 20 |
NC_000003.12 |
3 |
178427765 |
+ |
LINC01014 |
NNNCCTTGGAAGATGTACAG |
NGG |
2 |
0.0265 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
34719869 |
+ |
Grk4 |
NNNGCTTGGAAGATTTACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000069.6 |
3 |
154807232 |
+ |
Tnni3k |
NNNGCTTGGAAGATTAACAA |
NGG |
2 |
0.8523 |
Tier II |
| 3 |
NC_000086.7 |
X |
96141445 |
+ |
Msn |
NNNGCTTGGAAAATTTATAG |
NGG |
2 |
0.6 |
Tier II |
| 4 |
NC_000073.6 |
7 |
57762837 |
- |
Gabrb3 |
NNNGCTTGGAAGGTTAACAG |
NGG |
2 |
0.5929 |
Tier II |
| 5 |
NC_000078.6 |
12 |
56830222 |
+ |
Slc25a21 |
NNNGCTTGGAACATTAACAG |
NGG |
2 |
0.4813 |
Tier II |
| 6 |
NC_000072.6 |
6 |
118927582 |
- |
Cacna1c |
NNNCCATGGAAGATTTACAG |
NGG |
2 |
0.4588 |
Tier II |
| 7 |
NC_000078.6 |
12 |
56763718 |
- |
Slc25a21 |
NNNCCATGGAAGATTTACAG |
NGG |
2 |
0.4588 |
Tier II |
| 8 |
NC_000071.6 |
5 |
74485059 |
+ |
Scfd2 |
NNNGATTGGAAGGTTTACAG |
NGG |
2 |
0.3727 |
Tier II |
| 9 |
NC_000084.6 |
18 |
68267309 |
- |
Fam210a |
NNNGGTTGGAAGAATTACAG |
NGG |
2 |
0.3714 |
Tier II |
| 10 |
NC_000067.6 |
1 |
55528515 |
- |
Plcl1 |
NNNCCTTGAAAGATTTACAG |
NGG |
2 |
0.3403 |
Tier II |
| 11 |
NC_000080.6 |
14 |
67798643 |
- |
Dock5 |
NNNACTTGGCAGATTTACAG |
NGG |
2 |
0.3 |
Tier II |
| 12 |
NC_000077.6 |
11 |
75861048 |
+ |
Rph3al |
NNNGCTTGGAAGATTAACAG |
NAG |
2 |
0.2357 |
Tier II |
| 13 |
NC_000076.6 |
10 |
28152042 |
+ |
Ptprk |
NNNGCTTTGCAGATTTACAG |
NGG |
2 |
0.2083 |
Tier II |
| 14 |
NC_000078.6 |
12 |
111542568 |
+ |
Eif5 |
NNNTATTGGAAGATTTACAG |
NGG |
2 |
0.2078 |
Tier II |
| 15 |
NC_000084.6 |
18 |
35457507 |
+ |
Sil1 |
NNNGCTTGGAAGATTGAAAG |
NGG |
2 |
0.1864 |
Tier II |
| 16 |
NC_000067.6 |
1 |
126802508 |
- |
Nckap5 |
NNNGCATGGAAGATTTCCAG |
NGG |
2 |
0.1529 |
Tier II |
| 17 |
NC_000071.6 |
5 |
19855496 |
- |
Magi2 |
NNNGTTTGGAAGATTTCCAG |
NGG |
2 |
0.1123 |
Tier II |
| 18 |
NC_000069.6 |
3 |
116945228 |
+ |
Palmd |
NNNGCTTGGAAGGTTTACAG |
NGA |
2 |
0.0453 |
Tier II |
| 19 |
NC_000072.6 |
6 |
77232026 |
- |
Ctnna2 |
NNNACTTGGAAGATTTACAG |
NTG |
2 |
0.0351 |
Tier II |
| 20 |
NC_000072.6 |
6 |
77232026 |
- |
Lrrtm1 |
NNNACTTGGAAGATTTACAG |
NTG |
2 |
0.0351 |
Tier II |
| 21 |
NC_000077.6 |
11 |
11386253 |
- |
Zpbp |
NNNGCTTGGAAGATTCACAG |
NTG |
2 |
0.026 |
Tier II |
| 22 |
NC_000077.6 |
11 |
55902026 |
- |
LOC105244170 |
NNNGCTTGTAAGATATACAG |
NGG |
2 |
0.3088 |
Tier III |
| 23 |
NC_000083.6 |
17 |
16965401 |
+ |
BC002059 |
NNNCCTTGGAAGATTTAAAG |
NGG |
2 |
0.2851 |
Tier III |
| 24 |
NC_000074.6 |
8 |
26429492 |
+ |
Gm31898 |
NNNGCTTGGAAGATTCTCAG |
NGG |
2 |
0.0889 |
Tier III |
| 25 |
NC_000070.6 |
4 |
124545876 |
- |
Gm2164 |
NNNGCTTGGAAGATTTTCAG |
NAG |
2 |
0.0346 |
Tier III |
| 26 |
NC_000067.6 |
1 |
177301477 |
+ |
Gm36904 |
NNNGATTGGAAGATTTACAG |
NGT |
2 |
0.0092 |
Tier III |
Other clones with same target sequence:
(none)
