Construct: sgRNA BRDN0001145223
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGAGCCGAGTGTTAGTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHB6 (2051)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77272
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
142866197 |
+ |
EPHB6 |
NNNGAGCCGAGTGTTAGTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
20693569 |
- |
LDAH |
NNNGAGCTGACTGTTAGTGG |
NGG |
2 |
0.375 |
Tier II |
| 3 |
NC_000008.11 |
8 |
448675 |
+ |
FBXO25 |
NNNGAGCAGAGTGTGAGTGG |
NGG |
2 |
0.0325 |
Tier II |
| 4 |
NC_000012.12 |
12 |
46268834 |
- |
SLC38A1 |
NNNGAGCAGAGTGTGAGTGG |
NGG |
2 |
0.0325 |
Tier II |
| 5 |
NC_000019.10 |
19 |
35052903 |
- |
HPN |
NNNGAGCCGGGTGTGAGTGG |
NGG |
2 |
0.0278 |
Tier II |
| 6 |
NC_000001.11 |
1 |
27552887 |
- |
AHDC1 |
NNNGAGCCGAGGGTGAGTGG |
NGG |
2 |
0.025 |
Tier II |
| 7 |
NC_000010.11 |
10 |
110304809 |
+ |
SMNDC1 |
NNNGAGCCCAGTGGTAGTGG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000001.11 |
1 |
29267905 |
+ |
PTPRU |
NNNGAGCCGAGTGGAAGTGG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000015.10 |
15 |
34724007 |
- |
LOC124903463 |
NNNGGGCTGAGTGTTAGTGG |
NGG |
2 |
0.63 |
Tier III |
| 10 |
NC_000003.12 |
3 |
42648834 |
+ |
ZBTB47-AS1 |
NNNTAGCTGAGTGTTAGTGG |
NGG |
2 |
0.3182 |
Tier III |
| 11 |
NC_000003.12 |
3 |
18006137 |
- |
BALR6 |
NNNGAGCAGAGTGTTAGTGG |
NAG |
2 |
0.1685 |
Tier III |
| 12 |
NC_000004.12 |
4 |
148935777 |
+ |
LOC107986195 |
NNNGAGCCCAGTGTTGGTGG |
NGG |
2 |
0.1036 |
Tier III |
| 13 |
NC_000001.11 |
1 |
792934 |
- |
LINC01409 |
NNNGAGCAGAGTGTGAGTGG |
NGG |
2 |
0.0325 |
Tier III |
| 14 |
NC_000001.11 |
1 |
792934 |
- |
LOC124903817 |
NNNGAGCAGAGTGTGAGTGG |
NGG |
2 |
0.0325 |
Tier III |
| 15 |
NC_000001.11 |
1 |
224017841 |
- |
SEPTIN7P13 |
NNNGAGCAGAGTGTGAGTGG |
NGG |
2 |
0.0325 |
Tier III |
| 16 |
NC_000015.10 |
15 |
96354348 |
- |
LOC101927263 |
NNNGAGCCGAGTGTGGGTGG |
NGG |
2 |
0.0096 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
41616148 |
+ |
Ephb6 |
NNNGAGTCGAGTGTTAGTTG |
NGG |
2 |
0.5417 |
Tier I |
| 2 |
NC_000067.6 |
1 |
160195551 |
+ |
Mrps14 |
NNNGAGCCGAGTGTGAGTGG |
NGA |
2 |
0.0035 |
Tier I |
| 3 |
NC_000074.6 |
8 |
70569563 |
+ |
Ell |
NNNGAGCCATGTGTTAGTGG |
NGG |
2 |
0.5672 |
Tier II |
| 4 |
NC_000083.6 |
17 |
35553767 |
+ |
Cdsn |
NNNGAGCAGAGAGTTAGTGG |
NGG |
2 |
0.52 |
Tier II |
| 5 |
NC_000067.6 |
1 |
69665574 |
- |
Ikzf2 |
NNNGAGGTGAGTGTTAGTGG |
NGG |
2 |
0.4118 |
Tier II |
| 6 |
NC_000083.6 |
17 |
23573402 |
- |
Zfp13 |
NNNGAGCCGAGTCTTAATGG |
NGG |
2 |
0.393 |
Tier II |
| 7 |
NC_000077.6 |
11 |
83186113 |
- |
Slfn4 |
NNNGAGCCCAGTGTTAGTGC |
NGG |
2 |
0.2308 |
Tier II |
| 8 |
NC_000078.6 |
12 |
117254041 |
- |
Ptprn2 |
NNNGAGCAGAGTGTTGGTGG |
NGG |
2 |
0.125 |
Tier II |
| 9 |
NC_000077.6 |
11 |
108787048 |
- |
Cep112 |
NNNGAGCCCAGTGTTAGTGG |
NGT |
2 |
0.0087 |
Tier II |
| 10 |
NC_000067.6 |
1 |
191307557 |
+ |
Nenf |
NNNGAGCAGAGTGTTTGTGG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000071.6 |
5 |
67690468 |
- |
Atp8a1 |
NNNGAGCCGAGTGTTAGTGG |
NCA |
2 |
0.0 |
Tier II |
| 12 |
NC_000077.6 |
11 |
82918569 |
+ |
Unc45b |
NNNGAGCCGGGTGGTAGTGG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000078.6 |
12 |
117254041 |
- |
Gm5441 |
NNNGAGCAGAGTGTTGGTGG |
NGG |
2 |
0.125 |
Tier III |
| 14 |
NC_000075.6 |
9 |
42239040 |
- |
Gm40515 |
NNNGAGCCGAGTGTTAGTAG |
NGA |
2 |
0.0496 |
Tier III |
| 15 |
NC_000080.6 |
14 |
47935450 |
+ |
4930447J18Rik |
NNNGAGCCGAGTGAGAGTGG |
NGG |
2 |
0.031 |
Tier III |
Other clones with same target sequence:
(none)
