Construct: sgRNA BRDN0001145230
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTCCCCTATGATCACCAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDGFRB (5159)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77061
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
150134854 |
- |
PDGFRB |
NNNCCCTATGATCACCAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
18162678 |
- |
MYO15A |
NNNCCCTATGCTCACCAACA |
NGG |
2 |
0.375 |
Tier I |
| 3 |
NC_000002.12 |
2 |
110393550 |
+ |
LIMS4 |
NNNCCCTGTGGTCACCAACG |
NGG |
2 |
0.4767 |
Tier II |
| 4 |
NC_000005.10 |
5 |
172844792 |
- |
ERGIC1 |
NNNCCCTAGGATCACCAAAG |
NGG |
2 |
0.2653 |
Tier II |
| 5 |
NC_000001.11 |
1 |
8613926 |
+ |
RERE |
NNNCCCTATGATCACCCACA |
NGG |
2 |
0.1654 |
Tier II |
| 6 |
NC_000004.12 |
4 |
78817593 |
+ |
BMP2K |
NNNCCCTATGTTCACCAACC |
NGG |
2 |
0.1319 |
Tier II |
| 7 |
NC_000003.12 |
3 |
46708957 |
- |
TMIE |
NNNCCCTATGATGACCAAGG |
NGG |
2 |
0.017 |
Tier II |
| 8 |
NC_000013.11 |
13 |
40219867 |
+ |
LINC00548 |
NNNCCCTGTGATCACCAACA |
NGG |
2 |
0.6875 |
Tier III |
| 9 |
NC_000024.10 |
Y |
23472917 |
- |
REREP1Y |
NNNCCCTATGATCACCCACA |
NGG |
2 |
0.1654 |
Tier III |
| 10 |
NC_000024.10 |
Y |
26197695 |
+ |
REREP2Y |
NNNCCCTATGATCACCCACA |
NGG |
2 |
0.1654 |
Tier III |
| 11 |
NC_000004.12 |
4 |
78817593 |
+ |
LOC105377299 |
NNNCCCTATGTTCACCAACC |
NGG |
2 |
0.1319 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
122821141 |
- |
Anapc5 |
NNNCCCGATGATGACCAACG |
NGG |
2 |
0.0802 |
Tier I |
| 2 |
NC_000068.7 |
2 |
91131409 |
+ |
Mybpc3 |
NNNCCCTAAGATCAGCAACG |
NGG |
2 |
0.0464 |
Tier I |
| 3 |
NC_000083.6 |
17 |
73116052 |
- |
Lclat1 |
NNNCACTATGATCACCAACC |
NGG |
2 |
0.2449 |
Tier II |
| 4 |
NC_000078.6 |
12 |
101866862 |
+ |
Trip11 |
NNNCCCTATGTTCACCAACC |
NGG |
2 |
0.1319 |
Tier II |
| 5 |
NC_000073.6 |
7 |
130246193 |
- |
Fgfr2 |
NNNCCCTAGGATCACCAAGG |
NGG |
2 |
0.0774 |
Tier II |
| 6 |
NC_000071.6 |
5 |
63598649 |
- |
Gm30837 |
NNNTCCTATGATCACCAAAG |
NGG |
2 |
0.3429 |
Tier III |
Other clones with same target sequence:
(none)
