Construct: sgRNA BRDN0001145237
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATTGAAGTGGAGCCAACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KSR2 (283455)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76427
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
117527079 |
- |
KSR2 |
NNNTGAAGTGGAGCCAACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
121509893 |
+ |
KDM2B |
NNNTGAAGTGGAGCCATCGG |
NGG |
2 |
0.0235 |
Tier I |
| 3 |
NC_000008.11 |
8 |
53718236 |
+ |
ATP6V1H |
NNNTGAAGTGGGGCCAAAGT |
NGG |
2 |
0.3889 |
Tier II |
| 4 |
NC_000012.12 |
12 |
570152 |
+ |
NINJ2 |
NNNTGAAATGGAGCCAGCGT |
NGG |
2 |
0.1765 |
Tier II |
| 5 |
NC_000008.11 |
8 |
104390134 |
- |
DPYS |
NNNTGAAGTGGATCCAAGGT |
NGG |
2 |
0.04 |
Tier II |
| 6 |
NC_000011.10 |
11 |
132920392 |
+ |
OPCML |
NNNTGAAGTGGAGAGAACGT |
NGG |
2 |
0.0175 |
Tier II |
| 7 |
NC_000020.11 |
20 |
42931546 |
+ |
PTPRT |
NNNAGAAGTGGAGCCTACGT |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
47945869 |
+ |
Csmd3 |
NNNTGAAGTGTAGCCAACCT |
NGG |
2 |
0.1724 |
Tier II |
| 2 |
NC_000068.7 |
2 |
153625799 |
- |
Commd7 |
NNNTGAAGTGGAGCCAATGG |
NGG |
2 |
0.1134 |
Tier II |
| 3 |
NC_000075.6 |
9 |
25495985 |
- |
Eepd1 |
NNNTGAAGTGGAGCCTGCGT |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
