Construct: sgRNA BRDN0001145242
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTCAACGGGATACCAGTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MPP7 (143098)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75832
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
28124069 |
- |
MPP7 |
NNNCAACGGGATACCAGTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
153869809 |
- |
L1CAM |
NNNCAACGGGATCCCTGTGG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000006.12 |
6 |
38676477 |
+ |
GLO1 |
NNNCAACAGGAAACCAGTGG |
NGG |
2 |
0.8 |
Tier II |
| 4 |
NC_000002.12 |
2 |
241384031 |
- |
FARP2 |
NNNCAGCGGGATAACAGTGG |
NGG |
2 |
0.25 |
Tier II |
| 5 |
NC_000001.11 |
1 |
214604166 |
+ |
CENPF |
NNNCAACGGGAAAGCAGTGG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000005.10 |
5 |
172623683 |
+ |
LOC107986478 |
NNNCAAGGGGACACCAGTGG |
NGG |
2 |
0.4458 |
Tier III |
| 7 |
NC_000003.12 |
3 |
170716469 |
+ |
SLC7A14-AS1 |
NNNCAACGGGATGCCAGTGG |
NAG |
2 |
0.1691 |
Tier III |
| 8 |
NC_000003.12 |
3 |
170716469 |
+ |
LOC124906302 |
NNNCAACGGGATGCCAGTGG |
NAG |
2 |
0.1691 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
85288680 |
+ |
Zc2hc1c |
NNNCAACGGGATAACAGAGG |
NGG |
2 |
0.2333 |
Tier II |
Other clones with same target sequence:
(none)
