Construct: sgRNA BRDN0001145245
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGGTGGCCAGGATCATGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MPP3 (4356)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77810
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
43827777 |
- |
MPP3 |
NNNGTGGCCAGGATCATGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
201685090 |
- |
MPP4 |
NNNGTGGCCAGGATCATCCA |
NGG |
2 |
0.4464 |
Tier I |
| 3 |
NC_000010.11 |
10 |
28125036 |
- |
MPP7 |
NNNGTGGCCAGAATCATGAG |
NGG |
2 |
0.4 |
Tier I |
| 4 |
NC_000001.11 |
1 |
94072129 |
+ |
ABCA4 |
NNNGTGGCCAGGAACATGCA |
NGG |
2 |
0.5804 |
Tier II |
| 5 |
NC_000011.10 |
11 |
121095866 |
- |
TBCEL-TECTA |
NNNGTGGCCAGGAACATGCT |
NGG |
2 |
0.4333 |
Tier II |
| 6 |
NC_000014.9 |
14 |
93999144 |
- |
CCDC197 |
NNNCTGGCCAGGGTCATGCG |
NGG |
2 |
0.3453 |
Tier II |
| 7 |
NC_000004.12 |
4 |
150784435 |
- |
LRBA |
NNNGTTGCCAGGATCATGTG |
NGG |
2 |
0.3077 |
Tier II |
| 8 |
NC_000003.12 |
3 |
51678291 |
- |
TEX264 |
NNNCTGGCCAGGATCAAGCG |
NGG |
2 |
0.2824 |
Tier II |
| 9 |
NC_000019.10 |
19 |
48981673 |
+ |
GYS1 |
NNNGAGGCCAGGATCATGTG |
NGG |
2 |
0.2308 |
Tier II |
| 10 |
NC_000016.10 |
16 |
31331436 |
+ |
ITGAM |
NNNGTGGCCGGGTTCATGCG |
NGG |
2 |
0.1667 |
Tier II |
| 11 |
NC_000006.12 |
6 |
89312940 |
+ |
GABRR2 |
NNNGTGCCCAGGCTCATGCG |
NGG |
2 |
0.1447 |
Tier II |
| 12 |
NC_000002.12 |
2 |
85369957 |
+ |
ELMOD3 |
NNNGTGCCCAGGATCATGGG |
NGG |
2 |
0.0859 |
Tier II |
| 13 |
NC_000001.11 |
1 |
27546591 |
- |
AHDC1 |
NNNGTGGCCAGGAGCATGTG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000001.11 |
1 |
94072129 |
+ |
LOC124904222 |
NNNGTGGCCAGGAACATGCA |
NGG |
2 |
0.5804 |
Tier III |
| 15 |
NC_000017.11 |
17 |
16832093 |
- |
KRT16P2 |
NNNGTGGCCAGGATCATCCA |
NGG |
2 |
0.4464 |
Tier III |
| 16 |
NC_000017.11 |
17 |
20503763 |
- |
KRT16P3 |
NNNGTGGCCAGGATCATCCA |
NGG |
2 |
0.4464 |
Tier III |
| 17 |
NC_000014.9 |
14 |
104249272 |
+ |
LINC02691 |
NNNGTGGCCAGGATCAGGCA |
NGG |
2 |
0.1103 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
102020498 |
- |
Mpp3 |
NNNGTGGCCAGGATCATGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000084.6 |
18 |
7442819 |
- |
Mpp7 |
NNNGTGGCCCGAATCATGCG |
NGG |
2 |
0.3111 |
Tier I |
| 3 |
NC_000070.6 |
4 |
107514642 |
+ |
Glis1 |
NNNGTGGCCAGGGTCATACG |
NGG |
2 |
0.4515 |
Tier II |
| 4 |
NC_000082.6 |
16 |
22009494 |
- |
Senp2 |
NNNGTAGCCAGGATCAGGCG |
NGG |
2 |
0.1176 |
Tier II |
| 5 |
NC_000071.6 |
5 |
124816217 |
- |
Dnah10 |
NNNGTGGCCAGGCTCATGAG |
NGG |
2 |
0.0902 |
Tier II |
| 6 |
NC_000074.6 |
8 |
25910417 |
- |
Kcnu1 |
NNNGTGGCCAGGATCATGGG |
NCG |
2 |
0.0134 |
Tier II |
| 7 |
NC_000082.6 |
16 |
33938066 |
+ |
Itgb5 |
NNNGTGGCCAGGAGCATGCT |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
