Construct: sgRNA BRDN0001145246
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATTTGTATGGAATACTGTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP4K5 (11183)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75710
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
50485610 |
- |
MAP4K5 |
NNNTGTATGGAATACTGTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
43511377 |
+ |
ZNF487 |
NNNTGTGTGGAATACTGTGA |
NGG |
2 |
0.6618 |
Tier II |
| 3 |
NC_000023.11 |
X |
69866887 |
+ |
EDA |
NNNTGTGTGGAATACTGTGA |
NGG |
2 |
0.6618 |
Tier II |
| 4 |
NC_000016.10 |
16 |
19458541 |
- |
TMC5 |
NNNTGCCTGGAATACTGTGG |
NGG |
2 |
0.3977 |
Tier II |
| 5 |
NC_000001.11 |
1 |
201686295 |
- |
NAV1 |
NNNTGGATGGAATACTGAGG |
NGG |
2 |
0.381 |
Tier II |
| 6 |
NC_000009.12 |
9 |
92354121 |
+ |
CENPP |
NNNTGTATGGCATACTGTGA |
NGG |
2 |
0.375 |
Tier II |
| 7 |
NC_000014.9 |
14 |
103630789 |
+ |
KLC1 |
NNNTGTATGGAATTCTGTGT |
NGG |
2 |
0.3733 |
Tier II |
| 8 |
NC_000016.10 |
16 |
47139164 |
- |
NETO2 |
NNNTGTATTGAATACTGTTG |
NGG |
2 |
0.3556 |
Tier II |
| 9 |
NC_000006.12 |
6 |
64418979 |
+ |
EYS |
NNNTGTCTGGAGTACTGTGG |
NGG |
2 |
0.316 |
Tier II |
| 10 |
NC_000008.11 |
8 |
10117042 |
+ |
MSRA |
NNNTGTATGGAATAATGTGA |
NGG |
2 |
0.2083 |
Tier II |
| 11 |
NC_000003.12 |
3 |
36868055 |
+ |
TRANK1 |
NNNTGAATGGAATCCTGTGG |
NGG |
2 |
0.1857 |
Tier II |
| 12 |
NC_000016.10 |
16 |
9894753 |
+ |
GRIN2A |
NNNTGTCTGGCATACTGTGG |
NGG |
2 |
0.175 |
Tier II |
| 13 |
NC_000003.12 |
3 |
8766946 |
- |
OXTR |
NNNTGTATGGTTTACTGTGG |
NGG |
2 |
0.1026 |
Tier II |
| 14 |
NC_000011.10 |
11 |
30488421 |
- |
MPPED2 |
NNNTGTATGGAAGAATGTGG |
NGG |
2 |
0.058 |
Tier II |
| 15 |
NC_000022.11 |
22 |
26207224 |
- |
SEZ6L |
NNNTGAATGGAATACTGTGG |
NGC |
2 |
0.0193 |
Tier II |
| 16 |
NC_000006.12 |
6 |
136789538 |
- |
MAP3K5 |
NNNTGTATGGAATAGTGGGG |
NGG |
2 |
0.0167 |
Tier II |
| 17 |
NC_000010.11 |
10 |
124926014 |
+ |
ZRANB1 |
NNNTGTATTGAATACTGTGG |
NGT |
2 |
0.0086 |
Tier II |
| 18 |
NC_000006.12 |
6 |
125092549 |
+ |
RNF217 |
NNNTGTATGGATTACTGTGG |
NGT |
2 |
0.0054 |
Tier II |
| 19 |
NC_000001.11 |
1 |
108240308 |
- |
NBPF4 |
NNNTGTATGGAATACTGTGG |
NAC |
2 |
0.0 |
Tier II |
| 20 |
NC_000001.11 |
1 |
108454038 |
+ |
NBPF6 |
NNNTGTATGGAATACTGTGG |
NAC |
2 |
0.0 |
Tier II |
| 21 |
NC_000007.14 |
7 |
52389591 |
- |
LOC124901810 |
NNNTGTGTGGAATACTGTGA |
NGG |
2 |
0.6618 |
Tier III |
| 22 |
NC_000007.14 |
7 |
90109259 |
+ |
STEAP2-AS1 |
NNNTGTATGGAATACAGTGG |
NAG |
2 |
0.2357 |
Tier III |
| 23 |
NC_000005.10 |
5 |
118215754 |
+ |
LINC02147 |
NNNTGTATTGATTACTGTGG |
NGG |
2 |
0.1778 |
Tier III |
| 24 |
NC_000002.12 |
2 |
195123232 |
- |
LOC105376755 |
NNNTTTATGGAATAATGTGG |
NGG |
2 |
0.0667 |
Tier III |
| 25 |
NC_000018.10 |
18 |
80152138 |
+ |
PARD6G-AS1 |
NNNTGTATGGAAAACTGTGG |
NGA |
2 |
0.0481 |
Tier III |
| 26 |
NC_000023.11 |
X |
10886647 |
+ |
HCCS-DT |
NNNTGTATGGGATACTGTGG |
NTG |
2 |
0.0253 |
Tier III |
| 27 |
NC_000011.10 |
11 |
25543646 |
+ |
LINC02699 |
NNNTTTATGGAATACTGTGG |
NTG |
2 |
0.0117 |
Tier III |
| 28 |
NC_000001.11 |
1 |
108379577 |
+ |
NBPF5P |
NNNTGTATGGAATACTGTGG |
NAC |
2 |
0.0 |
Tier III |
| 29 |
NC_000009.12 |
9 |
86681686 |
+ |
LINC02834 |
NNNTGTATGGAATACTGTGG |
NCA |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
80655889 |
- |
Map4k3 |
NNNTGCATGGAATTCTGTGG |
NGG |
2 |
0.4848 |
Tier I |
| 2 |
NC_000085.6 |
19 |
6341936 |
+ |
Map4k2 |
NNNTGTATGGAGTTCTGTGG |
NGG |
2 |
0.3852 |
Tier I |
| 3 |
NC_000082.6 |
16 |
41099358 |
- |
Lsamp |
NNNTGTATGAAATACAGTGG |
NGG |
2 |
0.8485 |
Tier II |
| 4 |
NC_000075.6 |
9 |
7359878 |
- |
Mmp12 |
NNNTGTGTGGAATACTATGG |
NGG |
2 |
0.6588 |
Tier II |
| 5 |
NC_000078.6 |
12 |
74132462 |
- |
Syt16 |
NNNTGCATGGAATACTGAGG |
NGG |
2 |
0.6061 |
Tier II |
| 6 |
NC_000077.6 |
11 |
29768505 |
- |
Eml6 |
NNNAGTATGGAATACTGTGG |
NGG |
1 |
0.4762 |
Tier II |
| 7 |
NC_000080.6 |
14 |
123492264 |
+ |
Nalcn |
NNNTGGATGGAAAACTGTGG |
NGG |
2 |
0.3956 |
Tier II |
| 8 |
NC_000073.6 |
7 |
16865602 |
+ |
Prkd2 |
NNNTGTTTGGAATACTGAGG |
NGG |
2 |
0.2917 |
Tier II |
| 9 |
NC_000082.6 |
16 |
85456881 |
+ |
Cyyr1 |
NNNTGTTTGGAATACTGTTG |
NGG |
2 |
0.2917 |
Tier II |
| 10 |
NC_000076.6 |
10 |
62575819 |
+ |
Kifbp |
NNNTGTATGCAGTACTGTGG |
NGG |
2 |
0.2889 |
Tier II |
| 11 |
NC_000073.6 |
7 |
100742712 |
+ |
Fam168a |
NNNTGTTTGGGATACTGTGG |
NGG |
2 |
0.2844 |
Tier II |
| 12 |
NC_000072.6 |
6 |
6058792 |
- |
Slc25a13 |
NNNTGTCTTGAATACTGTGG |
NGG |
2 |
0.2333 |
Tier II |
| 13 |
NC_000072.6 |
6 |
39974549 |
+ |
Tmem178b |
NNNTGTATGGACAACTGTGG |
NGG |
2 |
0.1822 |
Tier II |
| 14 |
NC_000076.6 |
10 |
105768895 |
+ |
Mettl25 |
NNNTGTATAGAAGACTGTGG |
NGG |
2 |
0.1677 |
Tier II |
| 15 |
NC_000070.6 |
4 |
137528337 |
- |
Hspg2 |
NNNTGTATGGAAGCCTGTGG |
NGG |
2 |
0.0559 |
Tier II |
| 16 |
NC_000082.6 |
16 |
94889544 |
+ |
Kcnj6 |
NNNTGCATGGAATACTGTGG |
NTG |
2 |
0.0354 |
Tier II |
| 17 |
NC_000068.7 |
2 |
77663230 |
- |
Zfp385b |
NNNTGTATGGTATATTGTGG |
NGG |
2 |
0.0205 |
Tier II |
| 18 |
NC_000075.6 |
9 |
94679508 |
- |
Slc9a9 |
NNNTGTATGGAATACTGTAG |
NGC |
2 |
0.0159 |
Tier II |
| 19 |
NC_000071.6 |
5 |
100701969 |
+ |
Hpse |
NNNTGTATGCAATACTGTGG |
NTG |
2 |
0.0156 |
Tier II |
| 20 |
NC_000085.6 |
19 |
30986161 |
- |
Prkg1 |
NNNTGAATGGAATACTGTGG |
NGT |
2 |
0.014 |
Tier II |
| 21 |
NC_000084.6 |
18 |
37896020 |
- |
Diaph1 |
NNNTGTATGGAATAGTGTGG |
NGA |
2 |
0.0035 |
Tier II |
| 22 |
NC_000085.6 |
19 |
24791984 |
- |
Pgm5 |
NNNTGTATGGAATACTGTGG |
NTA |
2 |
0.0 |
Tier II |
| 23 |
NC_000068.7 |
2 |
110375249 |
- |
Gm30319 |
NNNCATATGGAATACTGTGG |
NGG |
2 |
0.5608 |
Tier III |
| 24 |
NC_000068.7 |
2 |
105620949 |
+ |
Pax6os1 |
NNNTGTATGGAAAACTGTGG |
NAG |
2 |
0.1795 |
Tier III |
| 25 |
NC_000069.6 |
3 |
58096689 |
+ |
Gm40056 |
NNNTATATGGAATACTGTGG |
NGC |
2 |
0.0193 |
Tier III |
Other clones with same target sequence:
(none)
