Construct: sgRNA BRDN0001145248
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGGTCGTGACAGTACTCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SIK1 (150094)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77895
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
6116537 |
- |
LOC102724428 |
NNNGTCGTGACAGTACTCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000021.9 |
21 |
43421725 |
+ |
SIK1 |
NNNGTCGTGACAGTACTCCA |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000019.10 |
19 |
18396603 |
+ |
LRRC25 |
NNNGTCGTGCCAGGACTCCA |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000009.12 |
9 |
97914720 |
+ |
TRMO |
NNNGTCGTGAAAGTTCTCCA |
NGG |
2 |
0.15 |
Tier II |
| 5 |
NC_000009.12 |
9 |
127050736 |
- |
RALGPS1 |
NNNGGCGTGACAGTACTCCA |
NGA |
2 |
0.0444 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
31851322 |
+ |
Sik1 |
NNNGTTGTGGCAGTACTCCA |
NGG |
2 |
0.5159 |
Tier I |
| 2 |
NC_000072.6 |
6 |
114828933 |
+ |
Atg7 |
NNNGTGGTGACAGTGCTCCA |
NGG |
2 |
0.325 |
Tier II |
| 3 |
NC_000082.6 |
16 |
4127222 |
+ |
Crebbp |
NNNGTCTTGACAGTACTCTA |
NGG |
2 |
0.2637 |
Tier II |
| 4 |
NC_000075.6 |
9 |
103570651 |
+ |
Tmem108 |
NNNGAGGTGACAGTACTCCA |
NGG |
2 |
0.25 |
Tier II |
| 5 |
NC_000069.6 |
3 |
37570124 |
+ |
Spata5 |
NNNGTCGTGCCAGTGCTCCA |
NGG |
2 |
0.2167 |
Tier II |
| 6 |
NC_000080.6 |
14 |
66154639 |
+ |
Ptk2b |
NNNGTGGTGACAGCACTCCA |
NGG |
2 |
0.1429 |
Tier II |
| 7 |
NC_000071.6 |
5 |
6892878 |
+ |
Zfp804b |
NNNCTCGTGACAGTACTCCA |
NTG |
2 |
0.0206 |
Tier II |
| 8 |
NC_000069.6 |
3 |
37570124 |
+ |
Gm36412 |
NNNGTCGTGCCAGTGCTCCA |
NGG |
2 |
0.2167 |
Tier III |
| 9 |
NC_000086.7 |
X |
59623029 |
- |
Gm36631 |
NNNGTCGTGACAGAATTCCA |
NGG |
2 |
0.1905 |
Tier III |
Other clones with same target sequence:
(none)
