Construct: sgRNA BRDN0001145252
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGCCCTCGACGGAGAGAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TTBK1 (84630)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75801
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
43263322 |
+ |
TTBK1 |
NNNCCCTCGACGGAGAGAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
131108059 |
- |
ADGRD1 |
NNNCCCAGGACGGAGAGAGT |
NGG |
2 |
0.5625 |
Tier II |
| 3 |
NC_000009.12 |
9 |
121645464 |
+ |
DAB2IP |
NNNCTCTCGACGGAGAGAGG |
NGG |
2 |
0.1123 |
Tier II |
| 4 |
NC_000020.11 |
20 |
29301471 |
- |
LOC107987277 |
NNNCCCTCGACGGGGTGAGT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
46467155 |
- |
Ttbk1 |
NNNACCTCGACGGAGAGAGT |
NAG |
2 |
0.2183 |
Tier I |
| 2 |
NC_000075.6 |
9 |
56721652 |
- |
Lingo1 |
NNNCCCACGAAGGAGAGAGT |
NGG |
2 |
0.6563 |
Tier II |
| 3 |
NC_000073.6 |
7 |
127218363 |
+ |
Septin1 |
NNNCCCTCGAGGGAGAGAGC |
NGG |
2 |
0.0227 |
Tier II |
| 4 |
NC_000073.6 |
7 |
144403979 |
- |
Shank2 |
NNNCCCTCGAGGGAGAGAGT |
NTG |
2 |
0.0097 |
Tier II |
| 5 |
NC_000073.6 |
7 |
144403979 |
- |
Gm14372 |
NNNCCCTCGAGGGAGAGAGT |
NTG |
2 |
0.0097 |
Tier III |
Other clones with same target sequence:
(none)
