Construct: sgRNA BRDN0001145253
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCTGTCCATCAATTCGCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA7 (2045)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77351
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
93259405 |
- |
EPHA7 |
NNNTGTCCATCAATTCGCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
133286226 |
+ |
TUBGCP2 |
NNNTGTCCATCAAATAGCCA |
NGG |
2 |
0.619 |
Tier II |
| 3 |
NC_000016.10 |
16 |
83384418 |
- |
CDH13 |
NNNTGTCCATAAATTCGCCT |
NGG |
2 |
0.45 |
Tier II |
| 4 |
NC_000008.11 |
8 |
102225200 |
+ |
RRM2B |
NNNTATCCATCAATTCTCCA |
NGG |
2 |
0.2167 |
Tier II |
| 5 |
NC_000001.11 |
1 |
167078288 |
- |
GPA33 |
NNNTGTCCATTAATTTGCCA |
NGG |
2 |
0.0947 |
Tier II |
| 6 |
NC_000009.12 |
9 |
138111267 |
+ |
CACNA1B |
NNNTGTCCATCAATTGGCAA |
NGG |
2 |
0.0659 |
Tier II |
| 7 |
NC_000016.10 |
16 |
83384418 |
- |
CDH13-AS1 |
NNNTGTCCATAAATTCGCCT |
NGG |
2 |
0.45 |
Tier III |
| 8 |
NC_000016.10 |
16 |
62832636 |
+ |
LOC102723560 |
NNNTGTCCATTAATTGGCCA |
NGG |
2 |
0.0473 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
28947603 |
+ |
Epha7 |
NNNTGTCCATCAATTCGCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000067.6 |
1 |
64916713 |
+ |
Plekhm3 |
NNNTGTCAATCAATTAGCCA |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000072.6 |
6 |
56196015 |
+ |
Pde1c |
NNNTGTGCATCAATTCGCCA |
NGG |
1 |
0.4706 |
Tier II |
| 4 |
NC_000071.6 |
5 |
72946820 |
- |
Slain2 |
NNNTGGCCATCAATACGCCA |
NGG |
2 |
0.3308 |
Tier II |
| 5 |
NC_000078.6 |
12 |
117531791 |
+ |
Rapgef5 |
NNNTGGCCATCAATTCCCCA |
NGG |
2 |
0.1345 |
Tier II |
Other clones with same target sequence:
(none)
