Construct: sgRNA BRDN0001145258
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCTTGTATATCCTACTCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MKNK2 (2872)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77763
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
2041919 |
- |
MKNK2 |
NNNTTGTATATCCTACTCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
114175967 |
+ |
ZBTB16 |
NNNTTGTGAATCCTACTCAG |
NGG |
2 |
0.681 |
Tier II |
| 3 |
NC_000003.12 |
3 |
11016865 |
- |
SLC6A1 |
NNNTTGTATGTCCTGCTCAG |
NGG |
2 |
0.3611 |
Tier II |
| 4 |
NC_000013.11 |
13 |
114327087 |
+ |
CHAMP1 |
NNNTTGTATATCCTACTCCT |
NGG |
2 |
0.1448 |
Tier II |
| 5 |
NC_000007.14 |
7 |
150463785 |
- |
GIMAP8 |
NNNTTGTATATGCCACTCAG |
NGG |
2 |
0.127 |
Tier II |
| 6 |
NC_000007.14 |
7 |
18548738 |
- |
HDAC9 |
NNNTTGTATATCCTCCTCAG |
NGA |
2 |
0.0189 |
Tier II |
| 7 |
NC_000011.10 |
11 |
100227882 |
- |
CNTN5 |
NNNTTGTATATCCTCCTCAG |
NGA |
2 |
0.0189 |
Tier II |
| 8 |
NC_000005.10 |
5 |
59986816 |
+ |
PDE4D |
NNNTTGTATATCCTAGGCAG |
NGG |
2 |
0.0181 |
Tier II |
| 9 |
NC_000003.12 |
3 |
100420241 |
+ |
LNP1 |
NNNTTGTATATCCTTCTCAG |
NGA |
2 |
0.0139 |
Tier II |
| 10 |
NC_000002.12 |
2 |
187228883 |
+ |
CALCRL-AS1 |
NNNTTGTATATCTTACTCAA |
NGG |
2 |
0.6563 |
Tier III |
| 11 |
NC_000003.12 |
3 |
11016865 |
- |
SLC6A1-AS1 |
NNNTTGTATGTCCTGCTCAG |
NGG |
2 |
0.3611 |
Tier III |
| 12 |
NC_000009.12 |
9 |
106542881 |
+ |
LOC107987108 |
NNNCTGTATATCCTCCTCAG |
NGG |
2 |
0.1765 |
Tier III |
| 13 |
NC_000023.11 |
X |
22537965 |
+ |
PTCHD1-AS |
NNNTTGTATTTCCTTCTCAG |
NGG |
2 |
0.1765 |
Tier III |
| 14 |
NC_000018.10 |
18 |
14597432 |
- |
LOC105372004 |
NNNTTGTATATCCTTCTCAC |
NGG |
2 |
0.0857 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
152003371 |
- |
Hsd17b10 |
NNNTTGTATCTCCTTCTCAG |
NGG |
2 |
0.0667 |
Tier I |
| 2 |
NC_000084.6 |
18 |
70086202 |
- |
Rab27b |
NNNTTGTATATACTACTCAA |
NGG |
2 |
0.6696 |
Tier II |
| 3 |
NC_000078.6 |
12 |
71050110 |
+ |
Arid4a |
NNNTTTTATTTCCTACTCAG |
NGG |
2 |
0.5882 |
Tier II |
| 4 |
NC_000070.6 |
4 |
20332672 |
- |
Nkain3 |
NNNTTTTATATCCTACTCAT |
NGG |
2 |
0.4667 |
Tier II |
| 5 |
NC_000081.6 |
15 |
59413611 |
- |
Nsmce2 |
NNNTTGTTTTTCCTACTCAG |
NGG |
2 |
0.3782 |
Tier II |
| 6 |
NC_000080.6 |
14 |
11020328 |
+ |
Fhit |
NNNTTGTATATCCTACTCAG |
NAG |
1 |
0.2593 |
Tier II |
| 7 |
NC_000075.6 |
9 |
72973404 |
- |
Dnaaf4 |
NNNTTGTATTTCCTACTCAG |
NAG |
2 |
0.2288 |
Tier II |
| 8 |
NC_000080.6 |
14 |
27738218 |
- |
Erc2 |
NNNTTGTATATCTTCCTCAG |
NGG |
2 |
0.1909 |
Tier II |
| 9 |
NC_000072.6 |
6 |
65125780 |
- |
Hpgds |
NNNTTCTATATCCTTCTCAG |
NGG |
2 |
0.1364 |
Tier II |
| 10 |
NC_000072.6 |
6 |
54108711 |
- |
Chn2 |
NNNTAGTATATCCTTCTCAG |
NGG |
2 |
0.1 |
Tier II |
| 11 |
NC_000077.6 |
11 |
23263717 |
- |
Xpo1 |
NNNATGTATATCCTAGTCAG |
NGG |
2 |
0.0733 |
Tier II |
| 12 |
NC_000067.6 |
1 |
182012875 |
- |
Enah |
NNNTTGTATATCCTACTGTG |
NGG |
2 |
0.0718 |
Tier II |
| 13 |
NC_000067.6 |
1 |
11793066 |
+ |
A830018L16Rik |
NNNTTGTATTTCCTACTCAG |
NGA |
2 |
0.0613 |
Tier II |
| 14 |
NC_000084.6 |
18 |
24932102 |
+ |
Fhod3 |
NNNTTGTATATCATAGTCAG |
NGG |
2 |
0.0592 |
Tier II |
| 15 |
NC_000067.6 |
1 |
10305913 |
+ |
Cpa6 |
NNNTTGTATATTCTACTCAG |
NGT |
2 |
0.0087 |
Tier II |
| 16 |
NC_000084.6 |
18 |
70086202 |
- |
Gm41770 |
NNNTTGTATATACTACTCAA |
NGG |
2 |
0.6696 |
Tier III |
| 17 |
NC_000082.6 |
16 |
60611328 |
+ |
Gm9017 |
NNNTTGTATATGCTACTTAG |
NGG |
2 |
0.2857 |
Tier III |
| 18 |
NC_000083.6 |
17 |
39872407 |
+ |
AY036118 |
NNNTTGTATATCTTCCTCAG |
NGG |
2 |
0.1909 |
Tier III |
| 19 |
NC_000079.6 |
13 |
28896997 |
- |
Gm34639 |
NNNTTGTATATGCTAGTCAG |
NGG |
2 |
0.0684 |
Tier III |
Other clones with same target sequence:
(none)
