Construct: sgRNA BRDN0001145264

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGGCTCCAAAGATCAACTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GSK3B (2932)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77281

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 119912741 + GSK3B NNNGCTCCAAAGATCAACTC NGG 0 1.0 Tier I
2 NC_000001.11 1 37873105 - INPP5B NNNGCTACAAAGCTCAACTC NGG 2 0.1579 Tier I
3 NC_000019.10 19 42234390 + GSK3A NNNGCTCCAAAGATGAGCTC NGG 2 0.0088 Tier I
4 NC_000012.12 12 64474294 - TBK1 NNNGCTCCAAAGATCAACTG NTG 2 0.0023 Tier I
5 NC_000001.11 1 116924794 + PTGFRN NNNGATCCAAAGATCAATTC NGG 2 0.3673 Tier II
6 NC_000017.11 17 20211286 + SPECC1 NNNGCACCAAATATCAACTC NGG 2 0.3333 Tier II
7 NC_000015.10 15 89301721 - FANCI NNNGCACCAAAGATCAACAC NGG 2 0.2476 Tier II
8 NC_000017.11 17 7838115 - KDM6B NNNGCTCCAGAGATCAACAC NGG 2 0.1587 Tier II
9 NC_000017.11 17 7838115 - LOC124904106 NNNGCTCCAGAGATCAACAC NGG 2 0.1587 Tier II
10 NC_000016.10 16 31272221 + ITGAM NNNGCTACAAAGATCAGCTC NGG 2 0.1324 Tier II
11 NC_000016.10 16 66382789 + CDH5 NNNGCTCCAAGGATCACCTC NGG 2 0.1147 Tier II
12 NC_000010.11 10 96226319 + BLNK NNNGCTCAAAAGATCAACTG NGG 2 0.0382 Tier II
13 NC_000017.11 17 39255061 + FBXL20 NNNGCTCCAAAGATCAACTC NGC 1 0.0222 Tier II
14 NC_000002.12 2 50942182 - NRXN1 NNNGCTCCAAAGCTCAACTC NTG 2 0.0082 Tier II
15 NC_000015.10 15 80452397 + ARNT2 NNNGCTCCACAGATCAACTC NGT 2 0.0054 Tier II
16 NC_000006.12 6 46436160 - RCAN2 NNNGCACCAAAGAGCAACTC NGG 2 0.0 Tier II
17 NC_000011.10 11 67273330 - GRK2 NNNGCTCCCAAGATCTACTC NGG 2 0.0 Tier II
18 NC_000011.10 11 83425409 + CCDC90B-AS1 NNNCCTTCAAAGATCAACTC NGG 2 0.4301 Tier III
19 NC_000003.12 3 75675773 - LINC00960 NNNGCTGCAGAGATCAACTC NGG 2 0.2614 Tier III
20 NC_000020.11 20 29404905 + LOC105379476 NNNGCTGCAGAGATCAACTC NGG 2 0.2614 Tier III
21 NC_000015.10 15 89301721 - LOC124903548 NNNGCACCAAAGATCAACAC NGG 2 0.2476 Tier III
22 NC_000003.12 3 120363084 + BTNL12P NNNGCTCCAGAGTTCAACTC NGG 2 0.1667 Tier III
23 NC_000003.12 3 120363084 + LOC124906274 NNNGCTCCAGAGTTCAACTC NGG 2 0.1667 Tier III
24 NC_000003.12 3 164183696 + LOC102724419 NNNGCTCCAAAGCTCAACTC NTG 2 0.0082 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 38191598 - Gsk3b NNNGCTCCAAAGATCAACTC NGG 0 1.0 Tier I
2 NC_000076.6 10 82289910 - LOC102635990 NNNTCTCCAAAGATCAATTC NGG 2 0.2338 Tier I
3 NC_000077.6 11 98869540 + Wipf2 NNNGCTCCAAGGATCAACTC NGG 1 0.65 Tier II
4 NC_000080.6 14 98012787 + Dach1 NNNGGTTCAAAGATCAACTC NGG 2 0.4875 Tier II
5 NC_000070.6 4 129340768 - Zbtb8os NNNGTTCCAAAGAACAACTC NGG 2 0.3939 Tier II
6 NC_000077.6 11 62851953 - Fbxw10 NNNGCTTCAAAGATCAACTT NGG 2 0.2438 Tier II
7 NC_000085.6 19 4009626 - Ndufv1 NNNGCTCCAAAGTTCAACTT NGG 2 0.09 Tier II
8 NC_000085.6 19 58362758 + Gfra1 NNNGCTGCAAAGATCACCTC NGG 2 0.083 Tier II
9 NC_000070.6 4 151700941 + Camta1 NNNGCTGCAAAGATCAGCTC NGG 2 0.083 Tier II
10 NC_000079.6 13 105257063 + Rnf180 NNNGCTCCAATGCTCAACTC NGG 2 0.0648 Tier II
11 NC_000068.7 2 68359932 + Stk39 NNNGCTCCAAAGCTCAACAC NGG 2 0.0602 Tier II
12 NC_000085.6 19 10425173 - Syt7 NNNGGTCCAAAGATTAACTC NGG 2 0.04 Tier II
13 NC_000067.6 1 150898904 + Hmcn1 NNNGATCCAAAGATCAACTG NGG 2 0.0336 Tier II
14 NC_000083.6 17 22424251 + Zfp946 NNNGCTCCAAAGATCAAGTC NGA 2 0.0093 Tier II
15 NC_000077.6 11 114782296 - Kif19a NNNGCTCCAAAGAGCAGCTC NGG 2 0.0 Tier II
16 NC_000078.6 12 31933793 + Hbp1 NNNGCTCTAAAGAGCAACTC NGG 2 0.0 Tier II
17 NC_000078.6 12 31933793 + Cog5 NNNGCTCTAAAGAGCAACTC NGG 2 0.0 Tier II
18 NC_000070.6 4 151700941 + Gm38442 NNNGCTGCAAAGATCAGCTC NGG 2 0.083 Tier III
19 NC_000079.6 13 105257063 + Gm52064 NNNGCTCCAATGCTCAACTC NGG 2 0.0648 Tier III
Download CSV

Other clones with same target sequence:

(none)

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