Construct: sgRNA BRDN0001145264
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGGCTCCAAAGATCAACTC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GSK3B (2932)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77281
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
119912741 |
+ |
GSK3B |
NNNGCTCCAAAGATCAACTC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
37873105 |
- |
INPP5B |
NNNGCTACAAAGCTCAACTC |
NGG |
2 |
0.1579 |
Tier I |
| 3 |
NC_000019.10 |
19 |
42234390 |
+ |
GSK3A |
NNNGCTCCAAAGATGAGCTC |
NGG |
2 |
0.0088 |
Tier I |
| 4 |
NC_000012.12 |
12 |
64474294 |
- |
TBK1 |
NNNGCTCCAAAGATCAACTG |
NTG |
2 |
0.0023 |
Tier I |
| 5 |
NC_000001.11 |
1 |
116924794 |
+ |
PTGFRN |
NNNGATCCAAAGATCAATTC |
NGG |
2 |
0.3673 |
Tier II |
| 6 |
NC_000017.11 |
17 |
20211286 |
+ |
SPECC1 |
NNNGCACCAAATATCAACTC |
NGG |
2 |
0.3333 |
Tier II |
| 7 |
NC_000015.10 |
15 |
89301721 |
- |
FANCI |
NNNGCACCAAAGATCAACAC |
NGG |
2 |
0.2476 |
Tier II |
| 8 |
NC_000017.11 |
17 |
7838115 |
- |
KDM6B |
NNNGCTCCAGAGATCAACAC |
NGG |
2 |
0.1587 |
Tier II |
| 9 |
NC_000017.11 |
17 |
7838115 |
- |
LOC124904106 |
NNNGCTCCAGAGATCAACAC |
NGG |
2 |
0.1587 |
Tier II |
| 10 |
NC_000016.10 |
16 |
31272221 |
+ |
ITGAM |
NNNGCTACAAAGATCAGCTC |
NGG |
2 |
0.1324 |
Tier II |
| 11 |
NC_000016.10 |
16 |
66382789 |
+ |
CDH5 |
NNNGCTCCAAGGATCACCTC |
NGG |
2 |
0.1147 |
Tier II |
| 12 |
NC_000010.11 |
10 |
96226319 |
+ |
BLNK |
NNNGCTCAAAAGATCAACTG |
NGG |
2 |
0.0382 |
Tier II |
| 13 |
NC_000017.11 |
17 |
39255061 |
+ |
FBXL20 |
NNNGCTCCAAAGATCAACTC |
NGC |
1 |
0.0222 |
Tier II |
| 14 |
NC_000002.12 |
2 |
50942182 |
- |
NRXN1 |
NNNGCTCCAAAGCTCAACTC |
NTG |
2 |
0.0082 |
Tier II |
| 15 |
NC_000015.10 |
15 |
80452397 |
+ |
ARNT2 |
NNNGCTCCACAGATCAACTC |
NGT |
2 |
0.0054 |
Tier II |
| 16 |
NC_000006.12 |
6 |
46436160 |
- |
RCAN2 |
NNNGCACCAAAGAGCAACTC |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000011.10 |
11 |
67273330 |
- |
GRK2 |
NNNGCTCCCAAGATCTACTC |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000011.10 |
11 |
83425409 |
+ |
CCDC90B-AS1 |
NNNCCTTCAAAGATCAACTC |
NGG |
2 |
0.4301 |
Tier III |
| 19 |
NC_000003.12 |
3 |
75675773 |
- |
LINC00960 |
NNNGCTGCAGAGATCAACTC |
NGG |
2 |
0.2614 |
Tier III |
| 20 |
NC_000020.11 |
20 |
29404905 |
+ |
LOC105379476 |
NNNGCTGCAGAGATCAACTC |
NGG |
2 |
0.2614 |
Tier III |
| 21 |
NC_000015.10 |
15 |
89301721 |
- |
LOC124903548 |
NNNGCACCAAAGATCAACAC |
NGG |
2 |
0.2476 |
Tier III |
| 22 |
NC_000003.12 |
3 |
120363084 |
+ |
BTNL12P |
NNNGCTCCAGAGTTCAACTC |
NGG |
2 |
0.1667 |
Tier III |
| 23 |
NC_000003.12 |
3 |
120363084 |
+ |
LOC124906274 |
NNNGCTCCAGAGTTCAACTC |
NGG |
2 |
0.1667 |
Tier III |
| 24 |
NC_000003.12 |
3 |
164183696 |
+ |
LOC102724419 |
NNNGCTCCAAAGCTCAACTC |
NTG |
2 |
0.0082 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
38191598 |
- |
Gsk3b |
NNNGCTCCAAAGATCAACTC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000076.6 |
10 |
82289910 |
- |
LOC102635990 |
NNNTCTCCAAAGATCAATTC |
NGG |
2 |
0.2338 |
Tier I |
| 3 |
NC_000077.6 |
11 |
98869540 |
+ |
Wipf2 |
NNNGCTCCAAGGATCAACTC |
NGG |
1 |
0.65 |
Tier II |
| 4 |
NC_000080.6 |
14 |
98012787 |
+ |
Dach1 |
NNNGGTTCAAAGATCAACTC |
NGG |
2 |
0.4875 |
Tier II |
| 5 |
NC_000070.6 |
4 |
129340768 |
- |
Zbtb8os |
NNNGTTCCAAAGAACAACTC |
NGG |
2 |
0.3939 |
Tier II |
| 6 |
NC_000077.6 |
11 |
62851953 |
- |
Fbxw10 |
NNNGCTTCAAAGATCAACTT |
NGG |
2 |
0.2438 |
Tier II |
| 7 |
NC_000085.6 |
19 |
4009626 |
- |
Ndufv1 |
NNNGCTCCAAAGTTCAACTT |
NGG |
2 |
0.09 |
Tier II |
| 8 |
NC_000085.6 |
19 |
58362758 |
+ |
Gfra1 |
NNNGCTGCAAAGATCACCTC |
NGG |
2 |
0.083 |
Tier II |
| 9 |
NC_000070.6 |
4 |
151700941 |
+ |
Camta1 |
NNNGCTGCAAAGATCAGCTC |
NGG |
2 |
0.083 |
Tier II |
| 10 |
NC_000079.6 |
13 |
105257063 |
+ |
Rnf180 |
NNNGCTCCAATGCTCAACTC |
NGG |
2 |
0.0648 |
Tier II |
| 11 |
NC_000068.7 |
2 |
68359932 |
+ |
Stk39 |
NNNGCTCCAAAGCTCAACAC |
NGG |
2 |
0.0602 |
Tier II |
| 12 |
NC_000085.6 |
19 |
10425173 |
- |
Syt7 |
NNNGGTCCAAAGATTAACTC |
NGG |
2 |
0.04 |
Tier II |
| 13 |
NC_000067.6 |
1 |
150898904 |
+ |
Hmcn1 |
NNNGATCCAAAGATCAACTG |
NGG |
2 |
0.0336 |
Tier II |
| 14 |
NC_000083.6 |
17 |
22424251 |
+ |
Zfp946 |
NNNGCTCCAAAGATCAAGTC |
NGA |
2 |
0.0093 |
Tier II |
| 15 |
NC_000077.6 |
11 |
114782296 |
- |
Kif19a |
NNNGCTCCAAAGAGCAGCTC |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000078.6 |
12 |
31933793 |
+ |
Hbp1 |
NNNGCTCTAAAGAGCAACTC |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000078.6 |
12 |
31933793 |
+ |
Cog5 |
NNNGCTCTAAAGAGCAACTC |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000070.6 |
4 |
151700941 |
+ |
Gm38442 |
NNNGCTGCAAAGATCAGCTC |
NGG |
2 |
0.083 |
Tier III |
| 19 |
NC_000079.6 |
13 |
105257063 |
+ |
Gm52064 |
NNNGCTCCAATGCTCAACTC |
NGG |
2 |
0.0648 |
Tier III |
Other clones with same target sequence:
(none)
