Construct: sgRNA BRDN0001145268
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATAGTCTGTTATTACCTGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PI4KA (5297)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76816
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
20803193 |
+ |
PI4KA |
NNNGTCTGTTATTACCTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
25504861 |
+ |
GPR158 |
NNNGTCAGCTATTACCTGTG |
NGG |
2 |
0.8077 |
Tier II |
| 3 |
NC_000012.12 |
12 |
124015676 |
- |
ZNF664-RFLNA |
NNNGTCATTTATTACCTGTG |
NGG |
2 |
0.5469 |
Tier II |
| 4 |
NC_000005.10 |
5 |
177707191 |
- |
FAM153A |
NNNTTCTGTAATTACCTGTG |
NGG |
2 |
0.3117 |
Tier II |
| 5 |
NC_000004.12 |
4 |
20907536 |
- |
KCNIP4 |
NNNGTCTGTTATTGCCAGTG |
NGG |
2 |
0.2489 |
Tier II |
| 6 |
NC_000012.12 |
12 |
72320816 |
+ |
TRHDE |
NNNGTCTGTTACTAGCTGTG |
NGG |
2 |
0.0474 |
Tier II |
| 7 |
NC_000016.10 |
16 |
6691187 |
+ |
RBFOX1 |
NNNGTCTGCTATTAGCTGTG |
NGG |
2 |
0.0462 |
Tier II |
| 8 |
NC_000022.11 |
22 |
40118441 |
- |
TNRC6B |
NNNGTCAGTTATTAGCTGTG |
NGG |
2 |
0.0438 |
Tier II |
| 9 |
NC_000004.12 |
4 |
5792536 |
- |
EVC |
NNNGTCTGTTATTTTCTGTG |
NGG |
2 |
0.0356 |
Tier II |
| 10 |
NC_000019.10 |
19 |
7264707 |
+ |
INSR |
NNNGTCTTTTATTAGCTGTG |
NGG |
2 |
0.0313 |
Tier II |
| 11 |
NC_000020.11 |
20 |
15444715 |
+ |
MACROD2 |
NNNGTCTCTTATTAGCTGTG |
NGG |
2 |
0.0308 |
Tier II |
| 12 |
NC_000013.11 |
13 |
102342003 |
- |
FGF14 |
NNNGTCTGTTATTACTTGTG |
NTG |
2 |
0.012 |
Tier II |
| 13 |
NC_000005.10 |
5 |
176130978 |
+ |
LOC107986487 |
NNNTTCTGTAATTACCTGTG |
NGG |
2 |
0.3117 |
Tier III |
| 14 |
NC_000005.10 |
5 |
177707191 |
- |
LOC107986489 |
NNNTTCTGTAATTACCTGTG |
NGG |
2 |
0.3117 |
Tier III |
| 15 |
NC_000003.12 |
3 |
5349708 |
+ |
LOC124906205 |
NNNGTCTGTTTTTATCTGTG |
NGG |
2 |
0.0205 |
Tier III |
| 16 |
NC_000013.11 |
13 |
102342003 |
- |
FGF14-IT1 |
NNNGTCTGTTATTACTTGTG |
NTG |
2 |
0.012 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
14248970 |
- |
Mrc1 |
NNNTTCTGTCATTACCTGTG |
NGG |
2 |
0.1939 |
Tier I |
| 2 |
NC_000074.6 |
8 |
109624143 |
- |
Pkd1l3 |
NNNGTCTGATATTACCTGTG |
NTG |
2 |
0.0362 |
Tier I |
| 3 |
NC_000086.7 |
X |
160949090 |
+ |
Cdkl5 |
NNNGAATGTTATTACCTGTG |
NGG |
2 |
0.4643 |
Tier II |
| 4 |
NC_000076.6 |
10 |
120790469 |
- |
Msrb3 |
NNNGTCTGATATTGCCTGTG |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000078.6 |
12 |
76289912 |
+ |
Mthfd1 |
NNNGTCTGTTATCACCAGTG |
NGG |
2 |
0.4211 |
Tier II |
| 6 |
NC_000077.6 |
11 |
50092419 |
- |
Rnf130 |
NNNGCCTGTTATTACTTGTG |
NGG |
2 |
0.3077 |
Tier II |
| 7 |
NC_000075.6 |
9 |
59813777 |
+ |
Myo9a |
NNNGTCTGTTAGTACCAGTG |
NGG |
2 |
0.2667 |
Tier II |
| 8 |
NC_000067.6 |
1 |
38306065 |
- |
Aff3 |
NNNGTCTGTTGTTACCTGGG |
NGG |
2 |
0.1625 |
Tier II |
| 9 |
NC_000067.6 |
1 |
132490275 |
+ |
Rbbp5 |
NNNGTCTGTTATTAGATGTG |
NGG |
2 |
0.05 |
Tier II |
| 10 |
NC_000078.6 |
12 |
9595907 |
+ |
Gm40268 |
NNNGTCTGATATTACCTCTG |
NGG |
2 |
0.4422 |
Tier III |
| 11 |
NC_000069.6 |
3 |
87967695 |
+ |
Gm3745 |
NNNGTCTCTTATTGCCTGTG |
NGG |
2 |
0.2872 |
Tier III |
| 12 |
NC_000081.6 |
15 |
80398738 |
+ |
Gm41363 |
NNNGTCTGTTATTACCAGGG |
NGG |
2 |
0.1333 |
Tier III |
Other clones with same target sequence:
(none)
